Large dense-core vesicle exocytosis in pancreatic $beta;-cells monitored by capacitance measurements

Methods ◽  
2004 ◽  
Vol 33 (4) ◽  
pp. 302-311 ◽  
Author(s):  
T KANNO
Keyword(s):  
Beta Cells ◽  
Pancreatic Beta Cells ◽  
Dense Core ◽  
Dense Core Vesicle ◽  
Capacitance Measurements ◽  
Large Dense Core Vesicle ◽  
Vesicle Exocytosis

scholarly journals Modulation of large dense core vesicle insulin content mediates rhythmic hormone release from pancreatic beta cells over the 24h cycle

PLoS ONE ◽  
2018 ◽  
Vol 13 (3) ◽  
pp. e0193882
Author(s):  
Aurore Quinault ◽  
Corinne Leloup ◽  
Geoffrey Denwood ◽  
Coralie Spiegelhalter ◽  
Marianne Rodriguez ◽  
...  
Keyword(s):  
Beta Cells ◽  
Pancreatic Beta Cells ◽  
Dense Core ◽  
Insulin Content ◽  
Dense Core Vesicle ◽  
Hormone Release ◽  
Large Dense Core Vesicle

scholarly journals Examining Synaptotagmin 1 Function in Dense Core Vesicle Exocytosis under Direct Control of Ca2+

2003 ◽  
Vol 122 (3) ◽  
pp. 265-276 ◽  
Author(s):  
Jakob B. Sørensen ◽  
Rafael Fernández-Chacón ◽  
Thomas C. Südhof ◽  
Erwin Neher
Keyword(s):  
Dense Core ◽  
Dense Core Vesicle ◽  
Direct Control ◽  
Capacitance Measurements ◽  
Twofold Increase ◽  
Phospholipid Binding ◽  
Caged Calcium ◽  
Vesicle Exocytosis ◽  
Synaptotagmin 1 ◽  
Mutant Cells

We tested the long-standing hypothesis that synaptotagmin 1 is the Ca2+ sensor for fast neurosecretion by analyzing the intracellular Ca2+ dependence of large dense-core vesicle exocytosis in a mouse strain carrying a mutated synaptotagmin C2A domain. The mutation (R233Q) causes a twofold increase in the KD of Ca2+-dependent phospholipid binding to the double C2A-C2B domain of synaptotagmin. Using photolysis of caged calcium and capacitance measurements we found that secretion from mutant cells had lower secretory rates, longer secretory delays, and a higher intracellular Ca2+-threshold for secretion due to a twofold increase in the apparent KD of the Ca2+ sensor for fast exocytosis. Single amperometric fusion events were unchanged. We conclude that Ca2+-dependent phospholipid binding to synaptotagmin 1 mirrors the intracellular Ca2+ dependence of exocytosis.

scholarly journals Identification of a Munc13-sensitive step in chromaffin cell large dense-core vesicle exocytosis

eLife ◽  
2015 ◽  
Vol 4 ◽  
Author(s):  
Kwun Nok M Man ◽  
Cordelia Imig ◽  
Alexander M Walter ◽  
Paulo S Pinheiro ◽  
David R Stevens ◽  
...  
Keyword(s):  
Mathematical Model ◽  
Synaptic Vesicle ◽  
Chromaffin Cells ◽  
Chromaffin Cell ◽  
Dense Core ◽  
Dense Core Vesicle ◽  
Vesicle Docking ◽  
Large Dense Core Vesicle ◽  
Vesicle Exocytosis

It is currently unknown whether the molecular steps of large dense-core vesicle (LDCV) docking and priming are identical to the corresponding reactions in synaptic vesicle (SV) exocytosis. Munc13s are essential for SV docking and priming, and we systematically analyzed their role in LDCV exocytosis using chromaffin cells lacking individual isoforms. We show that particularly Munc13-2 plays a fundamental role in LDCV exocytosis, but in contrast to synapses lacking Munc13s, the corresponding chromaffin cells do not exhibit a vesicle docking defect. We further demonstrate that ubMunc13-2 and Munc13-1 confer Ca2+-dependent LDCV priming with similar affinities, but distinct kinetics. Using a mathematical model, we identify an early LDCV priming step that is strongly dependent upon Munc13s. Our data demonstrate that the molecular steps of SV and LDCV priming are very similar while SV and LDCV docking mechanisms are distinct.

Large Dense-Core Vesicle Exocytosis in PC12 Cells

Methods ◽  
1998 ◽  
Vol 16 (2) ◽  
pp. 204-208 ◽  
Author(s):  
Vadim A. Klenchin ◽  
Judith A. Kowalchyk ◽  
Thomas F.J. Martin
Keyword(s):  
Pc12 Cells ◽  
Dense Core ◽  
Dense Core Vesicle ◽  
Large Dense Core Vesicle ◽  
Vesicle Exocytosis

scholarly journals Author response: Identification of a Munc13-sensitive step in chromaffin cell large dense-core vesicle exocytosis

2015 ◽  
Author(s):  
Kwun Nok M Man ◽  
Cordelia Imig ◽  
Alexander M Walter ◽  
Paulo S Pinheiro ◽  
David R Stevens ◽  
...  
Keyword(s):  
Chromaffin Cell ◽  
Dense Core ◽  
Author Response ◽  
Dense Core Vesicle ◽  
Large Dense Core Vesicle ◽  
Vesicle Exocytosis
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