Large Dense-Core Vesicle Exocytosis in PC12 Cells

Methods ◽  
1998 ◽  
Vol 16 (2) ◽  
pp. 204-208 ◽  
Author(s):  
Vadim A. Klenchin ◽  
Judith A. Kowalchyk ◽  
Thomas F.J. Martin
Keyword(s):  
Pc12 Cells ◽  
Dense Core ◽  
Dense Core Vesicle ◽  
Large Dense Core Vesicle ◽  
Vesicle Exocytosis

scholarly journals Role of the polybasic sequence in the Doc2α C2B domain in dense-core vesicle exocytosis in PC12 cells

2010 ◽  
pp. no-no ◽  
Author(s):  
Mai Sato ◽  
Yasunori Mori ◽  
Takahide Matsui ◽  
Ryo Aoki ◽  
Manami Oya ◽  
...  
Keyword(s):  
Pc12 Cells ◽  
Dense Core ◽  
Dense Core Vesicle ◽  
Vesicle Exocytosis

Synaptotagmin function in dense core vesicle exocytosis studied in cracked PC12 cells

10.1038/nn869 ◽  
2002 ◽  
Vol 5 (7) ◽  
pp. 649-656 ◽  
Author(s):  
Ok-Ho Shin ◽  
Josep Rizo ◽  
Thomas C. Südhof
Keyword(s):  
Pc12 Cells ◽  
Dense Core ◽  
Dense Core Vesicle ◽  
Vesicle Exocytosis

Role of Doc2α in dense-core vesicle exocytosis in PC12 cells revealed by live cell imaging

2011 ◽  
Vol 71 ◽  
pp. e213
Author(s):  
Takashi Tsuboi ◽  
Yasunori Mori ◽  
Hideki Matsui ◽  
Ryo Aoki ◽  
Manami Oya ◽  
...  
Keyword(s):  
Pc12 Cells ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Dense Core ◽  
Dense Core Vesicle ◽  
Vesicle Exocytosis

scholarly journals RNA interference-mediated silencing of synaptotagmin IX, but not synaptotagmin I, inhibits dense-core vesicle exocytosis in PC12 cells

2004 ◽  
Vol 380 (3) ◽  
pp. 875-879 ◽  
Author(s):  
Mitsunori FUKUDA
Keyword(s):  
Rna Interference ◽  
Pc12 Cells ◽  
Small Interfering Rna ◽  
Dense Core ◽  
Dense Core Vesicle ◽  
Secretory Vesicles ◽  
Synaptotagmin I ◽  
Immunoaffinity Purification ◽  
Vesicle Exocytosis ◽  
Interfering Rna

Although PC12 cells express three synaptotagmin isoforms (Syts I, IV and IX), all of which have been proposed to regulate dense-core vesicle exocytosis, it remains unknown which of the Sytisoforms acts as the major Ca2+ sensor for dense-core vesicle exocytosis. In the present study, it has been shown by immunoaffinity purification and immunocytochemistry that Syts I and IX, but not Syt IV, are present on the same secretory vesicles in PC12 cells. Silencing of Syt IX with specific small interfering RNA significantly reduced high KCl-dependent neuropeptide Y secretion from PC12 cells, whereas silencing of Syt I with specific small interfering RNA had no significant effect. The results indicate that Syts I and IX are not functionally equivalent and that Syt IX, and not Syt I, is indispensable for the regulation of Ca2+-dependent dense-core vesicle exocytosis in PC12 cells.

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