scholarly journals Comparison Between Perfadex and Locally Manufactured Low-Potassium Dextran Solution for Pulmonary Preservation in an Ex Vivo Isolated Lung Perfusion Model

2011 ◽  
Vol 43 (1) ◽  
pp. 84-88 ◽  
Author(s):  
P.R.O. Soares ◽  
K.A.d.O. Braga ◽  
N.A. Nepomuceno ◽  
R. Pazetti ◽  
A.T. Correia ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Lung Perfusion ◽  
Perfusion Model ◽  
Isolated Lung Perfusion ◽  
Isolated Lung ◽  
Low Potassium ◽  
Dextran Solution

Characterization of local inflammatory response in an isolated lung perfusion model

1998 ◽  
Vol 5 (1) ◽  
pp. 87-92 ◽  
Author(s):  
Amir Abolhoda ◽  
Ari Brooks ◽  
Modassir Choudhry ◽  
Yoshikazu Kaneda ◽  
David Liu ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Lung Perfusion ◽  
Local Inflammatory Response ◽  
Perfusion Model ◽  
Isolated Lung Perfusion ◽  
Isolated Lung

scholarly journals Multitracer Stable Isotope Quantification of Arginase and Nitric Oxide Synthase Activity in a Mouse Model of Pseudomonas Lung Infection

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Hartmut Grasemann ◽  
Thomas Jaecklin ◽  
Anne Mehl ◽  
Hailu Huang ◽  
Mahroukh Rafii ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Stable Isotope ◽  
Mouse Model ◽  
Lung Infection ◽  
Lung Perfusion ◽  
Arginase Activity ◽  
Mouse Lung ◽  
Perfusion Model ◽  
Isolated Lung Perfusion ◽  
Isolated Lung

Cystic fibrosis airways are deficient for L-arginine, a substrate for nitric oxide synthases (NOSs) and arginases. The rationale for this study was to quantify NOS and arginase activity in the mouse lung. Anesthetized unventilated mice received a primed constant stable isotope intravenous infusion containing labeled L-arginine, ornithine, and citrulline. The isotopic enrichment of each of the infused isotopomers and its product amino acids were measured in plasma and organ homogenates using liquid chromatography-tandem mass spectrometry. The effect of infection was studied three days after direct tracheal instillation of Pseudomonas-coated agar beads. In the infusion model, lung infection resulted in a significant (28-fold) increase in NOS activity in lung but not in trachea, kidney, liver, or plasma. Absolute rates of arginase activity in solid tissues could not be calculated in this model. In an isolated lung perfusion model used for comparison increased NOS activity in infected lungs was confirmed (28.5-fold) and lung arginase activity was increased 9.7-fold. The activity of L-arginine metabolizing enzymes can be measured using stable isotope conversion in the mouse. Accumulation of L-ornithine in the whole mouse model hindered the exact quantification of arginase activity in the lung, a problem that was overcome utilizing an isolated lung perfusion model.

scholarly journals Implementation of an experimental isolated lung perfusion model on surgically resected human lobes

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Alexis Slama ◽  
Christian Raber ◽  
Celia Hedderich ◽  
Paul Stockhammer ◽  
Balazs Hegedüs ◽  
...  
Keyword(s):  
Lung Perfusion ◽  
Perfusion Model ◽  
Isolated Lung Perfusion ◽  
Isolated Lung

Effects of immediate versus delayed ex-vivo lung perfusion in a porcine cardiac arrest donation model

2019 ◽  
Vol 42 (7) ◽  
pp. 362-369 ◽  
Author(s):  
Carolin Olbertz ◽  
Nikolaus Pizanis ◽  
Hagen Bäumker ◽  
Simon Becker ◽  
Clemens Aigner ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Lung Perfusion ◽  
Large Animal Model ◽  
Large Animal ◽  
Ex Vivo Lung Perfusion ◽  
Promising Tool ◽  
Group I ◽  
Low Potassium ◽  
Dextran Solution ◽  
Circulatory Death

Objective:Ex-vivo lung perfusion is a promising tool to evaluate and recondition marginal donor lungs usually after a cold static preservation. The concept of continuous organ perfusion is supposed to reduce ischemic damage; however, the optimal perfusion protocol has not been established yet. The aim of this study was to compare immediate ex-vivo lung perfusion (I-EVLP) to delayed ex-vivo lung perfusion (D-EVLP) after a certain cold static preservation period on lung function in a large animal model.Methods:In a porcine model, lungs were procured after circulatory death and 60 min of no-touch warm ischemia. Lungs were preserved with single-flush cold low potassium dextran solution and prepared either for I-EVLP (n = 8) or stored cold for 9 h with subsequent D-EVLP (n = 8). Functional outcomes and morphology were compared during 4 h of ex-vivo lung perfusion, using STEEN SolutionTMas perfusion solution.Results:Pulmonary functional data, perfusate activities of lactate dehydrogenase, alkaline phosphatase, and products of lipid peroxidation did not differ significantly. There was a trend toward lower wet–dry ratio (I-EVLP: 13.4 ± 2.9; D-EVLP: 9.1 ± 2.5) and higher ΔpO2in D-EVLP group (I-EVLP: 209 ± 51.6 mmHg; D-EVLP: 236.3 ± 47.3 mmHg).Conclusion:In this donation-after-circulatory-death model, 9 h of cold static preservation followed by ex-vivo lung perfusion results in comparable pulmonary function to I-EVLP as indicated by oxygenation capacities and wet–dry ratio. Our findings indicate that prolonged cold static preservation prior to ex-vivo lung perfusion is as safe and effective as I-EVLP in the procurement of donor lungs.

scholarly journals Isolated Lung Perfusion in the Management of Acute Respiratory Distress Syndrome

2020 ◽  
Vol 21 (18) ◽  
pp. 6820
Author(s):  
Nathan Haywood ◽  
Matthew R. Byler ◽  
Aimee Zhang ◽  
Mark E. Roeser ◽  
Irving L. Kron ◽  
...  
Keyword(s):  
Acute Respiratory Distress Syndrome ◽  
Respiratory Distress Syndrome ◽  
Respiratory Distress ◽  
Distress Syndrome ◽  
Ex Vivo ◽  
Lung Perfusion ◽  
Ex Vivo Lung Perfusion ◽  
Isolated Lung Perfusion ◽  
Isolated Lung

Acute respiratory distress syndrome (ARDS) is associated with high morbidity and mortality, and current management has a dramatic impact on healthcare resource utilization. While our understanding of this disease has improved, the majority of treatment strategies remain supportive in nature and are associated with continued poor outcomes. There is a dramatic need for the development and breakthrough of new methods for the treatment of ARDS. Isolated machine lung perfusion is a promising surgical platform that has been associated with the rehabilitation of injured lungs and the induction of molecular and cellular changes in the lung, including upregulation of anti-inflammatory and regenerative pathways. Initially implemented in an ex vivo fashion to evaluate marginal donor lungs prior to transplantation, recent investigations of isolated lung perfusion have shifted in vivo and are focused on the management of ARDS. This review presents current tenants of ARDS management and isolated lung perfusion, with a focus on how ex vivo lung perfusion (EVLP) has paved the way for current investigations utilizing in vivo lung perfusion (IVLP) in the treatment of severe ARDS.

Sign in / Sign up

Export Citation Format

Share Document