Characterization of local inflammatory response in an isolated lung perfusion model

1998 ◽  
Vol 5 (1) ◽  
pp. 87-92 ◽  
Author(s):  
Amir Abolhoda ◽  
Ari Brooks ◽  
Modassir Choudhry ◽  
Yoshikazu Kaneda ◽  
David Liu ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Lung Perfusion ◽  
Local Inflammatory Response ◽  
Perfusion Model ◽  
Isolated Lung Perfusion ◽  
Isolated Lung

scholarly journals Multitracer Stable Isotope Quantification of Arginase and Nitric Oxide Synthase Activity in a Mouse Model of Pseudomonas Lung Infection

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Hartmut Grasemann ◽  
Thomas Jaecklin ◽  
Anne Mehl ◽  
Hailu Huang ◽  
Mahroukh Rafii ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Stable Isotope ◽  
Mouse Model ◽  
Lung Infection ◽  
Lung Perfusion ◽  
Arginase Activity ◽  
Mouse Lung ◽  
Perfusion Model ◽  
Isolated Lung Perfusion ◽  
Isolated Lung

Cystic fibrosis airways are deficient for L-arginine, a substrate for nitric oxide synthases (NOSs) and arginases. The rationale for this study was to quantify NOS and arginase activity in the mouse lung. Anesthetized unventilated mice received a primed constant stable isotope intravenous infusion containing labeled L-arginine, ornithine, and citrulline. The isotopic enrichment of each of the infused isotopomers and its product amino acids were measured in plasma and organ homogenates using liquid chromatography-tandem mass spectrometry. The effect of infection was studied three days after direct tracheal instillation of Pseudomonas-coated agar beads. In the infusion model, lung infection resulted in a significant (28-fold) increase in NOS activity in lung but not in trachea, kidney, liver, or plasma. Absolute rates of arginase activity in solid tissues could not be calculated in this model. In an isolated lung perfusion model used for comparison increased NOS activity in infected lungs was confirmed (28.5-fold) and lung arginase activity was increased 9.7-fold. The activity of L-arginine metabolizing enzymes can be measured using stable isotope conversion in the mouse. Accumulation of L-ornithine in the whole mouse model hindered the exact quantification of arginase activity in the lung, a problem that was overcome utilizing an isolated lung perfusion model.

scholarly journals Implementation of an experimental isolated lung perfusion model on surgically resected human lobes

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Alexis Slama ◽  
Christian Raber ◽  
Celia Hedderich ◽  
Paul Stockhammer ◽  
Balazs Hegedüs ◽  
...  
Keyword(s):  
Lung Perfusion ◽  
Perfusion Model ◽  
Isolated Lung Perfusion ◽  
Isolated Lung

scholarly journals Quality of Life Evolution after Pulmonary Metastasectomy: A Prospective Study Comparing Isolated Lung Perfusion with Standard Metastasectomy

2012 ◽  
Vol 7 (10) ◽  
pp. 1567-1573 ◽  
Author(s):  
Bram Balduyck ◽  
Jana Van Thielen ◽  
Anouschka Cogen ◽  
Willem Den Hengst ◽  
Jeroen Hendriks ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Prospective Study ◽  
Lung Perfusion ◽  
Pulmonary Metastasectomy ◽  
Isolated Lung Perfusion ◽  
Isolated Lung ◽  
A Prospective Study

scholarly journals Pharmacokinetics of paclitaxel administered by hyperthermic retrograde isolated lung perfusion techniques

2002 ◽  
Vol 123 (4) ◽  
pp. 686-694 ◽  
Author(s):  
David S. Schrump ◽  
Suoping Zhai ◽  
Dao M. Nguyen ◽  
Todd S. Weiser ◽  
Bradley A. Fisher ◽  
...  
Keyword(s):  
Lung Perfusion ◽  
Isolated Lung Perfusion ◽  
Isolated Lung
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