scholarly journals Low Incidence of High-Grade Pancreatic Intraepithelial Neoplasia Lesions in a Crmp4 Gene–Deficient Mouse Model of Pancreatic Cancer

2020 ◽  
Vol 13 (3) ◽  
pp. 100746
Author(s):  
Keiichi Yazawa ◽  
Fumio Nakamura ◽  
Daiki Masukawa ◽  
Sho Sato ◽  
Yukihiko Hiroshima ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Mouse Model ◽  
Intraepithelial Neoplasia ◽  
High Grade ◽  
Deficient Mouse ◽  
Pancreatic Intraepithelial Neoplasia ◽  
Low Incidence ◽  
Gene Deficient Mouse

scholarly journals Acinar transformed ductal cells exhibit differential mucin expression in a tamoxifen-induced pancreatic ductal adenocarcinoma mouse model

Biology Open ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. bio052878
Author(s):  
Kavita Mallya ◽  
Dhanya Haridas ◽  
Parthasarathy Seshacharyulu ◽  
Ramesh Pothuraju ◽  
Wade M. Junker ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Mouse Model ◽  
Pancreatic Ductal Adenocarcinoma ◽  
Intraepithelial Neoplasia ◽  
Ductal Adenocarcinoma ◽  
Chemotherapy Response ◽  
Pancreatic Intraepithelial Neoplasia ◽  
Ductal Cells ◽  
Mucin Expression ◽  
Acinar To Ductal Metaplasia

ABSTRACTPancreatic cancer (PC) is acquired postnatally; to mimic this scenario, we developed an inducible KrasG12D; Ptf1a-CreER™ (iKC) mouse model, in which Kras is activated postnatally at week 16 upon tamoxifen (TAM) administration. Upon TAM treatment, iKC mice develop pancreatic intraepithelial neoplasia (PanIN) lesions and PC with metastasis at the fourth and fortieth weeks, respectively, and exhibited acinar-to-ductal metaplasia (ADM) and transdifferentiation. Kras activation upregulated the transcription factors Ncoa3, p-cJun and FoxM1, which in turn upregulated expression of transmembrane mucins (Muc1, Muc4 and Muc16) and secretory mucin (Muc5Ac). Interestingly, knockdown of KrasG12D in multiple PC cell lines resulted in downregulation of MUC1, MUC4, MUC5AC and MUC16. In addition, iKC mice exhibited ADM and transdifferentiation. Our results show that the iKC mouse more closely mimics human PC development and can be used to investigate pancreatic ductal adenocarcinoma (PDAC) biomarkers, early onset of PDAC, and ADM. The iKC model can also be used for preclinical strategies such as targeting mucin axis alone or in combination with neo-adjuvant, immunotherapeutic approaches and to monitor chemotherapy response.

CC Chemokine Receptor 9 Enhances Proliferation in Pancreatic Intraepithelial Neoplasia and Pancreatic Cancer Cells

2009 ◽  
Vol 13 (11) ◽  
pp. 1955-1962 ◽  
Author(s):  
Xiaoming Shen ◽  
Brian Mailey ◽  
Joshua D. I. Ellenhorn ◽  
Peiguo G. Chu ◽  
Andrew M. Lowy ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Cancer Cells ◽  
Chemokine Receptor ◽  
Intraepithelial Neoplasia ◽  
Pancreatic Intraepithelial Neoplasia ◽  
Cc Chemokine ◽  
Pancreatic Cancer Cells

scholarly journals Molecular Characteristics of Pancreatic Ductal Adenocarcinomas with High-Grade Pancreatic Intraepithelial Neoplasia (PanIN) Are Different from Those without High-Grade PanIN

Pathobiology ◽  
2017 ◽  
Vol 84 (4) ◽  
pp. 192-201 ◽  
Author(s):  
Tetsuyuki Miyazaki ◽  
Yoshihiro Ohishi ◽  
Yoshihiro Miyasaka ◽  
Yasunori Oda ◽  
Shinichi Aishima ◽  
...  
Keyword(s):  
Intraepithelial Neoplasia ◽  
Molecular Characteristics ◽  
High Grade ◽  
Pancreatic Intraepithelial Neoplasia

scholarly journals Genetic analyses of isolated high-grade pancreatic intraepithelial neoplasia (HG-PanIN) reveal paucity of alterations in TP53 and SMAD4

2017 ◽  
Vol 242 (1) ◽  
pp. 16-23 ◽  
Author(s):  
Waki Hosoda ◽  
Peter Chianchiano ◽  
James F Griffin ◽  
Meredith E Pittman ◽  
Lodewijk AA Brosens ◽  
...  
Keyword(s):  
Intraepithelial Neoplasia ◽  
High Grade ◽  
Pancreatic Intraepithelial Neoplasia ◽  
Genetic Analyses
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