Antioxidative and oxidative changes in the digestive gland cells of freshwater mussels Unio tumidus caused by selected phenolic compounds in the presence of H2O2 or Cu2+ ions

2007 ◽  
Vol 21 (1) ◽  
pp. 146-156 ◽  
Author(s):  
Magdalena Labieniec ◽  
Teresa Gabryelak
Keyword(s):  
Phenolic Compounds ◽  
Digestive Gland ◽  
Freshwater Mussels ◽  
Gland Cells ◽  
Unio Tumidus

Biological Activity of Pentachlorophenol on the Digestive Gland Cells of the Freshwater Mussel Unio tumidus

2003 ◽  
Vol 58 (11-12) ◽  
pp. 867-872 ◽  
Author(s):  
Katarzyna Milowska ◽  
Teresa Gabryelak ◽  
Joanna Dudala ◽  
Magdalena Labieniec ◽  
Ekaterina Slobozhanina
Keyword(s):  
Plasma Membrane ◽  
Digestive Gland ◽  
Oxidative Dna Damage ◽  
Freshwater Mussel ◽  
Lipid Peroxides ◽  
Wood Preservative ◽  
Fluorescence Probes ◽  
Chlorinated Phenols ◽  
Gland Cells ◽  
Dna Strand

Abstract Many chlorinated phenols and their derivatives are used extensively as insecticides, fungicides and herbicides by industrial and agricultural users throughout the world. Among these substances, pentachlorophenol (PCP) is a broad-spectrum biocide, which is still used as a wood preservative. In this paper, the digestive gland cells were used to assess the effect of PCP in the range of concentrations 3.75-75 μᴍ (0.01-0.2 ppm) on oxidative DNA damage, fluidity changes and peroxidation activity in the plasma membrane. The toxic property of PCP on DNA strand breakage was studied using the comet assay. The results showed that pentachlorophenol in the range of 37.5-75 μᴍ contributed to these lesions. To demonstrate the changes in the fluidity of plasma membrane we used the spectrofluorimetric method using two fluorescence probes: 1-[4-(trimethylamino)phenyl]-6-phenyl-1,3,5-hexatriene (TMAD DPH) and 12-(9-anthroyloxy) stearic acid (12-AS). It was shown that PC did not influence the surface of plasma membrane but contributed to the increase in the fluidity of the internal region of the lipid bilayer in the range of concentrations 18.75-75 μᴍ (0.05-0.2 ppm).We also examined the effect of PCP on the lipid peroxidation. To imply its peroxidation properties the spectrophotometry method was used to measure the level of malondialdehyde (MDA), one of the endpoints of the peroxidation of polyunsaturated fatty acids. The obtained results showed that PCP in the used doses did not initiate the formation of lipid peroxides. Thus, our investigation indicates that PCP can behave as a prooxidant agent but its action depends on the used doses and parameters chosen for the research.

Possible role of lysosomes in the detoxication of copper in the digestive gland cells of metal-exposed mussels

1984 ◽  
Vol 14 (1-4) ◽  
pp. 469-470 ◽  
Author(s):  
A. Viarengo ◽  
M. Pertica ◽  
G. Mancinelli ◽  
M. Orunesu ◽  
G. Zanicchi ◽  
...  
Keyword(s):  
Digestive Gland ◽  
Gland Cells
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