Utilization of Mytilus digestive gland cells for the in vitro screening of potential metabolic disruptors in aquatic invertebrates

2017 ◽  
Vol 191 ◽  
pp. 26-35 ◽  
Author(s):  
Teresa Balbi ◽  
Caterina Ciacci ◽  
Elena Grasselli ◽  
Arianna Smerilli ◽  
Adriana Voci ◽  
...  
Keyword(s):  
Digestive Gland ◽  
Aquatic Invertebrates ◽  
In Vitro Screening ◽  
Gland Cells

In Vitro Screening of Azalea for Resistance to Azalea Lace Bug

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 506b-506
Author(s):  
Carol D. Robacker ◽  
S.K. Braman
Keyword(s):  
Leaf Damage ◽  
In Vitro Screening ◽  
Screening Assay ◽  
Delaware Valley ◽  
In Vitro Techniques ◽  
Culture Tube ◽  
Lace Bug ◽  
Lace Bugs ◽  
Laboratory Bioassays

Azalea lace bug (Stephanitis pyrioides) is the most serious pest on azalea. Results of laboratory bioassays and field evaluations of 17 deciduous azalea taxa have identified three resistant taxa: R. canescens, R. periclymenoides, and R. prunifolium. Highly susceptible taxa are `Buttercup', `My Mary', R. oblongifolium, and the evergreen cultivar `Delaware Valley White'. To determine whether in vitro techniques would have potential value in screening or selecting for resistance, or for the identification of morphological or chemical factors related to resistance, an in-vitro screening assay was developed. In-vitro shoot proliferation was obtained using the medium and procedures of Economou and Read (1984). Shoots used in the bioassays were grown in culture tubes. Two assays were developed: one for nymphs and one for adult lace bugs. To assay for resistance to nymphs, `Delaware Valley White' leaves containing lace bug eggs were disinfested with 70% alcohol and 20% commercial bleach, and incubated in sterile petri plates with moistened filter paper until the nymphs hatched. Five nymphs were placed in each culture tube, and cultures were incubated for about 2 weeks, or until adults were observed. To assay for resistance to adults, five female lace bugs were placed in each culture tube and allowed to feed for 5 days. Data collected on survival and leaf damage was generally supportive of laboratory bioassays and field results. Adult lace bugs had a low rate of survival on resistant taxa. Survival of nymphs was somewhat reduced on resistant taxa.

scholarly journals Thiosemicarbazole (Thiacetazone-Like) Compound with Activity against Mycobacterium avium in Mice

2003 ◽  
Vol 47 (8) ◽  
pp. 2685-2687 ◽  
Author(s):  
Luiz E. Bermudez ◽  
Robert Reynolds ◽  
Peter Kolonoski ◽  
Pricilla Aralar ◽  
Clark B. Inderlied ◽  
...  
Keyword(s):  
Mycobacterium Avium ◽  
Significant Activity ◽  
In Vitro Screening ◽  
Single Drug ◽  
Drug Regimens

ABSTRACT In vitro screening of thiacetazone derivatives indicated that two derivatives, SRI-286 and SRI-224, inhibited a panel of 25 Mycobacterium avium complex (MAC) isolates at concentrations of 2 μg/ml or lower. In mice, SRI-224 and thiacetazone had no significant activity against the MAC in livers and spleens, but treatment with SRI-286 resulted in significant reduction of bacterial loads in livers and spleens. A combination of SRI-286 and moxifloxacin was significantly more active than single drug regimens in liver and spleen.

Phytochemical composition and in vitro screening of the antimicrobial activity of essential oils on oral pathogenic bacteria

2017 ◽  
Vol 32 (5) ◽  
pp. 544-551 ◽  
Author(s):  
Roberta Tardugno ◽  
Federica Pellati ◽  
Ramona Iseppi ◽  
Moreno Bondi ◽  
Giacomo Bruzzesi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
In Vitro Screening ◽  
Phytochemical Composition

AN IN VITRO SCREENING ASSAY FOR INHIBITORS OF PROINFLAMMATORY MEDIATORS IN HERBAL EXTRACTS USING HUMAN SYNOVIOCYTE CULTURES

2004 ◽  
Vol 40 (3) ◽  
pp. 95 ◽  
Author(s):  
CARMELITA G. FRONDOZA ◽  
AFSHIN SOHRABI ◽  
ANNA POLOTSKY ◽  
PHONG V. PHAN ◽  
DAVID S. HUNGERFORD ◽  
...  
Keyword(s):  
In Vitro Screening ◽  
Herbal Extracts ◽  
Screening Assay ◽  
Proinflammatory Mediators
Sign in / Sign up

Export Citation Format

Share Document