Fluorescent probes for Pd2+ detection by poly(o-phenylenediamine) nanospheres with fluorescence enhancement

2012 ◽  
Vol 162 (5-6) ◽  
pp. 444-447 ◽  
Author(s):  
Zhoufeng Wang ◽  
Fang Liao
Keyword(s):  
Fluorescent Probes ◽  
Fluorescence Enhancement

Selective Detection of Mercury Ions Based on Tin Oxide Quantum Dots: Performance and Fluorescence Enhancement Model

2021 ◽  
Author(s):  
Jianqiao Liu ◽  
Yu Bai ◽  
Jingcheng Shi ◽  
Qing Yu ◽  
Jifei Liu ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Metal Ions ◽  
Tin Oxide ◽  
Fluorescent Probes ◽  
Fluorescence Enhancement ◽  
Selective Detection ◽  
Mercury Ions ◽  
Metal Ions Detection

The superiority of fluorescence-based method in the metal ions detection are well developed, however, the current quantum dots (QDs) fluorescent probes for mercury ions (Hg2+) still have many limitations, such...

scholarly journals Recent Advances on Iron(III) Selective Fluorescent Probes with Possible Applications in Bioimaging

Molecules ◽  
2019 ◽  
Vol 24 (18) ◽  
pp. 3267 ◽  
Author(s):  
Sahoo ◽  
Crisponi
Keyword(s):  
Fluorescent Probes ◽  
High Selectivity ◽  
Fluorescence Enhancement ◽  
Cost Effective ◽  
Fast Response ◽  
Vital Role ◽  
Molecular Probes ◽  
The Past ◽  
Environmental And Biological Samples ◽  
Almost All

Iron(III) is well-known to play a vital role in a variety of metabolic processes in almost all living systems, including the human body. However, the excess or deficiency of Fe3+ from the normal permissible limit can cause serious health problems. Therefore, novel analytical methods are developed for the simple, direct, and cost-effective monitoring of Fe3+ concentration in various environmental and biological samples. Because of the high selectivity and sensitivity, fast response time, and simplicity, the fluorescent-based molecular probes have been developed extensively in the past few decades to detect Fe3+. This review was narrated to summarize the Fe3+-selective fluorescent probes that show fluorescence enhancement (turn-on) and ratiometric response. The Fe3+ sensing ability, mechanisms along with the analytical novelties of recently reported 77 fluorescent probes are discussed.

A series of selective and sensitive fluorescent sensors based on a thiophen-2-yl-benzothiazole unit for Hg2+

2016 ◽  
Vol 40 (3) ◽  
pp. 2333-2342 ◽  
Author(s):  
Qunbo Mei ◽  
Ruqiang Tian ◽  
Yujie Shi ◽  
Qingfang Hua ◽  
Chen Chen ◽  
...  
Keyword(s):  
Fluorescence Quenching ◽  
Fluorescent Probe ◽  
Fluorescent Probes ◽  
Fluorescence Enhancement ◽  
Fluorescent Sensors ◽  
Benzothiazole Derivatives ◽  
Different Types ◽  
Quenching Probe ◽  
Ratiometric Fluorescent Probe

Different types of fluorescent probes for Hg2+ based on the 5-thiophen-2-yl-benzothiazole derivatives (TBT, CTBT, DTBT and NTBT) were realized by changing the subsituents, including the fluorescence quenching probe, the fluorescence enhancement probe and the ratiometric fluorescent probe.

Protein-induced fluorescence enhancement of two-photon excitable water-soluble diketopyrrolopyrroles

2017 ◽  
Vol 15 (31) ◽  
pp. 6511-6519 ◽  
Author(s):  
Simon Tang ◽  
Ebrahim H. Ghazvini Zadeh ◽  
Bosung Kim ◽  
Nikia T. Toomey ◽  
Mykhailo V. Bondar ◽  
...  
Keyword(s):  
Protein Binding ◽  
Fluorescent Probes ◽  
Fluorescence Enhancement ◽  
Water Soluble ◽  
High Yield ◽  
Two Photon ◽  
Fluorescence Switch

Water soluble diketopyrrolopyrrole one- and two-photon fluorescent probes were prepared in high yield exhibiting fluorescence switch-on upon protein binding.

Fluorescent Probes for Pd2+Detection by Allylidene–Hydrazone Ligands with Excellent Selectivity and Large Fluorescence Enhancement

2010 ◽  
Vol 16 (41) ◽  
pp. 12349-12356 ◽  
Author(s):  
Honglin Li ◽  
Jiangli Fan ◽  
Fengling Song ◽  
Hao Zhu ◽  
Jianjun Du ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Fluorescence Enhancement ◽  
Hydrazone Ligands ◽  
Excellent Selectivity

scholarly journals Multifunctional Thio-Stabilized Gold Nanoparticles for Near-Infrared Fluorescence Detection and Imaging of Activated Caspase-3

2021 ◽  
Vol 17 ◽  
Author(s):  
Jinda Fan ◽  
Philip P. Cheney ◽  
Sharon Bloch ◽  
Baogang Xu ◽  
Kexian Liang ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Fluorescent Probes ◽  
Caspase 3 ◽  
Near Infrared ◽  
Fluorescence Enhancement ◽  
Aqueous Media ◽  
Detection Sensitivity ◽  
Peptide Sequence ◽  
Nir Fluorescence ◽  
Fluorogenic Peptide

Background: Gold nanoparticles (AuNPs) are commonly used in nanomedicine because of their unique spectral properties, chemical and biological stability, and ability to quench the fluorescence of organic dyes attached to their surfaces. However, the utility of spherical AuNPs for activatable fluorescence sensing of molecular processes have been confined to resonance-matched fluorophores in the 500 nm to 600 nm spectral range to maximize dye fluorescence quenching efficiency. Expanding the repertoire of fluorophore systems into the NIR fluorescence regimen with emission >800 nm will facilitate the analysis of multiple biological events with high detection sensitivity. Objective: The primary goal of this study is to determine if spherical AuNP-induced radiative rate suppression of nonresonant near-infrared (NIR) fluorescent probes can serve as a versatile nanoconstruct for highly sensitive detection and imaging of activated caspase-3 in aqueous media and cancer cells. This required the development of activatable NIR fluorescence sensors of caspase-3 designed to overcome the nonspecific degradation and release of the surface coatings in aqueous media. Method: We harnessed the fluorescence-quenching properties and multivalency of spherical AuNPs to develop AuNPtemplated activatable NIR fluorescent probes to detect activated caspase-3, an intracellular reporter of early cell death. Freshly AuNPs were coated with a multifunctional NIR fluorescent dye-labeled peptide (LS422) consisting of an RGD peptide sequence that targets αvβ3-integrin protein (αvβ3) on the surface of cancer cells to mediate the uptake and internalization of the sensors in tumor cells; a DEVD peptide sequence for reporting the induction of cell death through caspase-3 mediated NIR fluorescence enhancement; and a multidentate hexacysteine sequence for enhancing selfassembly and stabilizing the multifunctional construct on AuNPs. The integrin binding affinity of LS422 and caspase-3 kinetics were determined by a radioligand competitive binding and fluorogenic peptide assays, respectively. Detection of intracellular caspase-3, cell viability, and the internalization of LS422 in cancer cells were determined by confocal NIR fluorescence spectroscopy and microscopy. Results: Narrow size AuNPs (13 nm) were prepared and characterized by transmission electron microscopy and dynamic light scattering. When assembled on the AuNPs, the binding constant of LS422 for αvβ3 improved 11-fold from 13.2 nM to 1.2 nM. Whereas the catalytic turnover of caspase-3 by LS422-AuNPs was similar to the reference fluorogenic peptide, the binding affinity for the enzyme increased by a factor of 2. Unlike the αvβ3 positive, but caspase-3 negative breast cancer MCF-7 cells, treatment of the αvβ3 and caspase-3 positive lung cancer A549 cells with Paclitaxel showed significant fluorescence enhancement within 30 minutes, which correlated with caspase-3 specific activation of LS422- AuNPs fluorescence. Incorporation of a 3.5 mW NIR laser source into our spectrofluorometer increased the detection sensitivity by an order of magnitude (limit of detection ~0.1 nM of cypate) and significantly decreased the signal noise relative to a xenon lamp. This gain in sensitivity enabled the detection of substrate hydrolysis at a broad range of inhibitor concentrations without photobleaching the cypate dye. Conclusion: The multifunctional AuNPs demonstrate the use of a non-resonant quenching strategy to design activatable NIR fluorescence molecular probes. The nanoconstruct offers a selective reporting method for detecting activated caspase-3, imaging of cell viability, identifying dying cells, and visualizing the functional status of intracellular enzymes. Performing these tasks with NIR fluorescent probes creates an opportunity to translate the in vitro and cellular analysis of enzymes into in vivo interrogation of their functional status using deep tissue penetrating NIR fluorescence analytical methods.

TQPHEN (N,N,N′,N′-tetrakis(2-quinolylmethyl)-1,2-phenylenediamine) derivatives as highly selective fluorescent probes for Cd2+

2015 ◽  
Vol 44 (1) ◽  
pp. 104-109 ◽  
Author(s):  
Yuji Mikata ◽  
Asako Kizu ◽  
Hideo Konno
Keyword(s):  
Fluorescent Probes ◽  
Fluorescence Enhancement ◽  
Specific Fluorescence

The tetrakisquinoline derivatives with a 1,2-phenylenediamine (PHEN) scaffold exhibit a Cd2+-specific fluorescence enhancement, in contrast to the Zn2+-specific trans-1,2-diaminocyclohexane (DACH) counterpart.

A novel fast-response and highly selective AIEgen fluorescent probe for visualizing peroxynitrite in living cells, C. elegans and inflammatory mice

The Analyst ◽  
2021 ◽  
Author(s):  
Ya-Xi Ye ◽  
Xin-Yue Chen ◽  
Ya-Wen Yu ◽  
Qing Zhang ◽  
Xiao-Wen Wei ◽  
...  
Keyword(s):  
Response Time ◽  
Fluorescent Probe ◽  
Fluorescent Probes ◽  
Fluorescence Enhancement ◽  
Fast Response ◽  
Living Cells ◽  
C Elegans

Most of ONOO− fluorescent probes have been restricted their further applications because of their aggregation-caused quenching (ACQ) effect, long response time and low fluorescence enhancement. Herein, we developed a novel...

Confocal microscopy of the cytoskeleton in living plant cells following microinjection of fluorescent probes

1993 ◽  
Vol 51 ◽  
pp. 130-131
Author(s):  
Ann Cleary
Keyword(s):  
Cell Division ◽  
Fluorescent Probes ◽  
Actin Filaments ◽  
Intracellular Transport ◽  
Cell Structure ◽  
Plant Cells ◽  
Cell Plate ◽  
Cell Cortex ◽  
Living Plant ◽  
Rhodamine Phalloidin

Microinjection of fluorescent probes into living plant cells reveals new aspects of cell structure and function. Microtubules and actin filaments are dynamic components of the cytoskeleton and are involved in cell growth, division and intracellular transport. To date, cytoskeletal probes used in microinjection studies have included rhodamine-phalloidin for labelling actin filaments and fluorescently labelled animal tubulin for incorporation into microtubules. From a recent study of Tradescantia stamen hair cells it appears that actin may have a role in defining the plane of cell division. Unlike microtubules, actin is present in the cell cortex and delimits the division site throughout mitosis. Herein, I shall describe actin, its arrangement and putative role in cell plate placement, in another material, living cells of Tradescantia leaf epidermis.The epidermis is peeled from the abaxial surface of young leaves usually without disruption to cytoplasmic streaming or cell division. The peel is stuck to the base of a well slide using 0.1% polyethylenimine and bathed in a solution of 1% mannitol +/− 1 mM probenecid.

Live Confocal Analysis of Fertilization and Early Development

2001 ◽  
Vol 7 (S2) ◽  
pp. 1012-1013
Author(s):  
Uyen Tram ◽  
William Sullivan
Keyword(s):  
Drosophila Melanogaster ◽  
Embryonic Development ◽  
Real Time ◽  
Early Development ◽  
Fluorescent Probes ◽  
Primary Defect ◽  
Fluorescent Analysis ◽  
Dynamic Event ◽  
Enormous Amount ◽  
Fluorescent Studies

Embryonic development is a dynamic event and is best studied in live animals in real time. Much of our knowledge of the early events of embryogenesis, however, comes from immunofluourescent analysis of fixed embryos. While these studies provide an enormous amount of information about the organization of different structures during development, they can give only a static glimpse of a very dynamic event. More recently real-time fluorescent studies of living embryos have become much more routine and have given new insights to how different structures and organelles (chromosomes, centrosomes, cytoskeleton, etc.) are coordinately regulated. This is in large part due to the development of commercially available fluorescent probes, GFP technology, and newly developed sensitive fluorescent microscopes. For example, live confocal fluorescent analysis proved essential in determining the primary defect in mutations that disrupt early nuclear divisions in Drosophila melanogaster. For organisms in which GPF transgenics is not available, fluorescent probes that label DNA, microtubules, and actin are available for microinjection.

Sign in / Sign up

Export Citation Format

Share Document