A sensitive electrochemical aptasensor for highly specific detection of streptomycin based on the porous carbon nanorods and multifunctional graphene nanocomposites for signal amplification

2017 ◽  
Vol 241 ◽  
pp. 151-159 ◽  
Author(s):  
Junling Yin ◽  
Wenjuan Guo ◽  
Xiaoli Qin ◽  
Juan Zhao ◽  
Meishan Pei ◽  
...  
Keyword(s):  
Porous Carbon ◽  
Signal Amplification ◽  
Electrochemical Aptasensor ◽  
Specific Detection ◽  
Graphene Nanocomposites

scholarly journals An electrochemical aptasensor for lead ion detection based on catalytic hairpin assembly and porous carbon supported platinum as signal amplification

RSC Advances ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 6647-6653
Author(s):  
Huali Jin ◽  
Di Zhang ◽  
Yong Liu ◽  
Min Wei
Keyword(s):  
Porous Carbon ◽  
Signal Amplification ◽  
Lead Ion ◽  
Electrochemical Aptasensor ◽  
Ion Detection ◽  
Supported Platinum ◽  
Lead Detection ◽  
Catalytic Hairpin Assembly

A novel electrochemical aptasensor for lead detection based on catalytic hairpin assembly and PtNPs@PCs as signal amplification.

Structure-Switching Electrochemical Aptasensor for Single-Step and Specific Detection of Trace Mercury in Dairy Products

2018 ◽  
Vol 66 (38) ◽  
pp. 10106-10112 ◽  
Author(s):  
Xinai Zhang ◽  
Chenyong Huang ◽  
Yanjuan Jiang ◽  
Yuxiang Jiang ◽  
Jianzhong Shen ◽  
...  
Keyword(s):  
Dairy Products ◽  
Single Step ◽  
Electrochemical Aptasensor ◽  
Specific Detection ◽  
Structure Switching

scholarly journals Single-ribonucleotide repair-mediated ligation-dependent cycling signal amplification for sensitive and specific detection of DNA methyltransferase

2018 ◽  
Vol 9 (28) ◽  
pp. 6053-6061 ◽  
Author(s):  
Li-juan Wang ◽  
Xiao Han ◽  
Chen-chen Li ◽  
Chun-yang Zhang
Keyword(s):  
Signal Amplification ◽  
Dna Methyltransferase ◽  
Sensitive Detection ◽  
Specific Detection ◽  
Mtase Activity

Specific and sensitive detection of DNA MTase activity can be achieved by a single-ribonucleotide repair-mediated ligation-dependent cycling signal amplification approach.

An electrochemical aptasensor based on cocoon-like DNA nanostructure signal amplification for the detection of Escherichia coli O157:H7

The Analyst ◽  
2020 ◽  
Vol 145 (22) ◽  
pp. 7340-7348
Author(s):  
Huasong Bai ◽  
Shengjun Bu ◽  
Wensen Liu ◽  
Chengyu Wang ◽  
Zhongyi Li ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Signal Amplification ◽  
Selective Detection ◽  
Escherichia Coli O157 ◽  
Dna Nanostructures ◽  
Electrochemical Aptasensor ◽  
Dna Nanostructure ◽  
Highly Sensitive

We developed an electrochemical aptasensor based on cocoon-like DNA nanostructures as signal tags for highly sensitive and selective detection of Escherichia coli O157:H7.

scholarly journals A novel electrochemical aptasensor for fumonisin B1 determination using DNA and exonuclease-I as signal amplification strategy

BMC Chemistry ◽  
2019 ◽  
Vol 13 (1) ◽  
Author(s):  
Min Wei ◽  
Fei Zhao ◽  
Shuo Feng ◽  
Huali Jin
Keyword(s):  
Incubation Time ◽  
Electrode Surface ◽  
Signal Amplification ◽  
Limit Of Detection ◽  
Fumonisin B1 ◽  
Electrochemical Aptasensor ◽  
Experimental Conditions ◽  
Exonuclease I ◽  
Double Stranded Dna ◽  
Amplification Strategy

Abstract In this work, using DNA and exonuclease-I (Exo-I) as signal amplification strategy, a novel and facile electrochemical aptasensor was constructed for fumonisin B1 (FB1) detection. The G-rich complementary DNA (cDNA) was immobilized onto the electrode surface. Then, aptamer of FB1 was hybridized with cDNA to form double-stranded DNA. In the absence of FB1, double-stranded DNA and G-rich cDNA on the electrode surface promoted effectively methylene blue (MB) enrichment and amplified the initial electrochemical response. In the presence of FB1, the combination of aptamer and FB1 led to the release of aptamer from the electrode surface and the expose of 3′ end of single-stranded cDNA. When Exo-I was added onto the electrode surface, the single-stranded cDNA was degraded in the 3′–5′ direction. The decrease of double-stranded DNA and G-rich cDNA resulted in the less access of MB to the electrode surface, which decreased the electrochemical signal. The experimental conditions including incubation time of FB1, the amount of Exo-I and incubation time of Exo-I were optimized. Under the optimal conditions, the linear relationship between the change of peak current and the logarithmic concentration of FB1 was observed in the range of 1.0 × 10−3–1000 ng mL−1 with a low limit of detection of 0.15 pg mL−1. The experimental results showed that the prepared aptasensor had acceptable specificity, reproducibility, repeatability and stability. Therefore, this proposed aptasensor has a potential application in the food safety detection.

Homogeneous electrochemical aptasensor based on a dual amplification strategy for sensitive detection of profenofos residues

2018 ◽  
Vol 42 (17) ◽  
pp. 14642-14647 ◽  
Author(s):  
Yancui Jiao ◽  
Jiayun Fu ◽  
Wenjie Hou ◽  
Zhaoqiang Shi ◽  
Yemin Guo ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Sensitive Detection ◽  
Homogeneous Type ◽  
Electrochemical Aptasensor ◽  
Dual Amplification ◽  
Amplification Strategy

A homogeneous type of electrochemical aptasensor was designed based upon the principle of target-induced and tool enzyme-assisted signal amplification, which was employed for the detection of profenofos residues.

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