scholarly journals An electrochemical aptasensor for lead ion detection based on catalytic hairpin assembly and porous carbon supported platinum as signal amplification

RSC Advances ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 6647-6653
Author(s):  
Huali Jin ◽  
Di Zhang ◽  
Yong Liu ◽  
Min Wei
Keyword(s):  
Porous Carbon ◽  
Signal Amplification ◽  
Lead Ion ◽  
Electrochemical Aptasensor ◽  
Ion Detection ◽  
Supported Platinum ◽  
Lead Detection ◽  
Catalytic Hairpin Assembly

A novel electrochemical aptasensor for lead detection based on catalytic hairpin assembly and PtNPs@PCs as signal amplification.

A label-free and enzyme-free signal amplification strategy for a sensitive RNase H activity assay

Nanoscale ◽  
2017 ◽  
Vol 9 (42) ◽  
pp. 16149-16153 ◽  
Author(s):  
Chang Yeol Lee ◽  
Hyowon Jang ◽  
Ki Soo Park ◽  
Hyun Gyu Park
Keyword(s):  
Signal Amplification ◽  
Rnase H ◽  
Label Free ◽  
Activity Assay ◽  
G Quadruplex ◽  
Catalytic Hairpin Assembly ◽  
Amplification Strategy

A target-triggered catalytic hairpin assembly with a G-quadruplex specific fluorescent binder, NMM, is employed to develop a novel and sensitive RNase H activity assay.

A FRET based aptasensor coupled with non-enzymatic signal amplification for mercury (II) ion detection

Talanta ◽  
2016 ◽  
Vol 155 ◽  
pp. 305-313 ◽  
Author(s):  
Ketsarin Chu-mong ◽  
Chongdee Thammakhet ◽  
Panote Thavarungkul ◽  
Proespichaya Kanatharana ◽  
Chittanon Buranachai
Keyword(s):  
Signal Amplification ◽  
Ion Detection ◽  
Enzymatic Signal Amplification

An electrochemical aptasensor based on cocoon-like DNA nanostructure signal amplification for the detection of Escherichia coli O157:H7

The Analyst ◽  
2020 ◽  
Vol 145 (22) ◽  
pp. 7340-7348
Author(s):  
Huasong Bai ◽  
Shengjun Bu ◽  
Wensen Liu ◽  
Chengyu Wang ◽  
Zhongyi Li ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Signal Amplification ◽  
Selective Detection ◽  
Escherichia Coli O157 ◽  
Dna Nanostructures ◽  
Electrochemical Aptasensor ◽  
Dna Nanostructure ◽  
Highly Sensitive

We developed an electrochemical aptasensor based on cocoon-like DNA nanostructures as signal tags for highly sensitive and selective detection of Escherichia coli O157:H7.

scholarly journals A novel electrochemical aptasensor for fumonisin B1 determination using DNA and exonuclease-I as signal amplification strategy

BMC Chemistry ◽  
2019 ◽  
Vol 13 (1) ◽  
Author(s):  
Min Wei ◽  
Fei Zhao ◽  
Shuo Feng ◽  
Huali Jin
Keyword(s):  
Incubation Time ◽  
Electrode Surface ◽  
Signal Amplification ◽  
Limit Of Detection ◽  
Fumonisin B1 ◽  
Electrochemical Aptasensor ◽  
Experimental Conditions ◽  
Exonuclease I ◽  
Double Stranded Dna ◽  
Amplification Strategy

Abstract In this work, using DNA and exonuclease-I (Exo-I) as signal amplification strategy, a novel and facile electrochemical aptasensor was constructed for fumonisin B1 (FB1) detection. The G-rich complementary DNA (cDNA) was immobilized onto the electrode surface. Then, aptamer of FB1 was hybridized with cDNA to form double-stranded DNA. In the absence of FB1, double-stranded DNA and G-rich cDNA on the electrode surface promoted effectively methylene blue (MB) enrichment and amplified the initial electrochemical response. In the presence of FB1, the combination of aptamer and FB1 led to the release of aptamer from the electrode surface and the expose of 3′ end of single-stranded cDNA. When Exo-I was added onto the electrode surface, the single-stranded cDNA was degraded in the 3′–5′ direction. The decrease of double-stranded DNA and G-rich cDNA resulted in the less access of MB to the electrode surface, which decreased the electrochemical signal. The experimental conditions including incubation time of FB1, the amount of Exo-I and incubation time of Exo-I were optimized. Under the optimal conditions, the linear relationship between the change of peak current and the logarithmic concentration of FB1 was observed in the range of 1.0 × 10−3–1000 ng mL−1 with a low limit of detection of 0.15 pg mL−1. The experimental results showed that the prepared aptasensor had acceptable specificity, reproducibility, repeatability and stability. Therefore, this proposed aptasensor has a potential application in the food safety detection.

FET Based Heavy Metal Ion Selective Sensors for Lead Ion Detection in Whole Blood

2019 ◽  
Vol 89 (6) ◽  
pp. 25-29
Author(s):  
Shin-Li Wang ◽  
Ching-Yen Hsieh ◽  
Yu-Lin Wang
Keyword(s):  
Heavy Metal ◽  
Whole Blood ◽  
Metal Ion ◽  
Heavy Metal Ion ◽  
Lead Ion ◽  
Ion Detection
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