Corrigendum to ‘Pregnancy derived from human zygote pronuclear transfer in a patient who had arrested embryos after IVF’ [Reproductive BioMedicine Online 33 (2016) 529–533]

2017 ◽  
Vol 34 (3) ◽  
pp. 284
Author(s):  
John Zhang ◽  
Guanglun Zhuang ◽  
Yong Zeng ◽  
Jamie Grifo ◽  
Carlo Acosta ◽  
...  
Keyword(s):  
Pronuclear Transfer

In vitro development of human triploid zygotes reconstructed by pronuclear transfer

2002 ◽  
Vol 78 ◽  
pp. S180-S181
Author(s):  
John Zhang ◽  
Yi Ming Shu ◽  
Lewis C Krey ◽  
Hui Liu ◽  
Guang Lun Zhuang ◽  
...  
Keyword(s):  
In Vitro Development ◽  
Pronuclear Transfer ◽  
Vitro Development

scholarly journals Precise allele-specific genome editing by spatiotemporal control of CRISPR-Cas9 via pronuclear transplantation

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Yanhe Li ◽  
Yuteng Weng ◽  
Dandan Bai ◽  
Yanping Jia ◽  
Yingdong Liu ◽  
...  
Keyword(s):  
Animal Models ◽  
Disease Model ◽  
Strand Break ◽  
Specific Gene ◽  
Genomic Change ◽  
Parental Allele ◽  
Allele Specific ◽  
Pronuclear Transfer ◽  
The Given ◽  
Spatiotemporal Control

Abstract Gene-targeted animal models that are generated by injecting Cas9 and sgRNAs into zygotes are often accompanied by undesired double-strand break (DSB)-induced byproducts and random biallelic targeting due to uncontrollable Cas9 targeting activity. Here, we establish a parental allele-specific gene-targeting (Past-CRISPR) method, based on the detailed observation that pronuclear transfer-mediated cytoplasmic dilution can effectively terminate Cas9 activity. We apply this method in embryos to efficiently target the given parental alleles of a gene of interest and observed little genomic mosaicism because of the spatiotemporal control of Cas9 activity. This method allows us to rapidly explore the function of individual parent-of-origin effects and to construct animal models with a single genomic change. More importantly, Past-CRISPR could also be used for therapeutic applications or disease model construction.

Can Maternal Spindle Transfer and Pronuclear Transfer Be Prohibited under eu Legislation?

2018 ◽  
Vol 25 (1) ◽  
pp. 57-74 ◽  
Author(s):  
Calum MacKellar
Keyword(s):  
Clinical Trials ◽  
Medicinal Product ◽  
Germ Line ◽  
Genetic Identity ◽  
Medical Procedure ◽  
Eu Legislation ◽  
Human Fertilisation ◽  
Mitochondrial Donation ◽  
Maternal Spindle Transfer ◽  
Pronuclear Transfer

Abstract The question whether maternal spindle transfer (mst) and pronuclear transfer (pnt) can be prohibited under eu legislation was examined by the non-governmental organisation European Bioethics Research (ebr). It did so by submitting an official complaint to the eu Commission proposing that the uk Human Fertilisation and Embryology (Mitochondrial Donation) Regulations 2015 breached the prohibition on the modification of a person’s germ line genetic identity of the eu Clinical Trials Directive 2001/20/EC and the new Regulation eu 536/2014. A discussion then took place, during 2016, between ebr and the eu Commission whether mst and pnt principally involved a ‘medicinal product’ in which case the eu Clinical Trials Directive 2001/20/EC and Regulation eu 536/2014 would be applicable or whether the procedures just involved a medical procedure in which case the Tissue and Cells Directive 2004/23/EC was applicable which did not include any prohibition on the intentional modification of a person’s germline.

A transgene insertional mutation at an imprinted locus in the mouse genome

Development ◽  
1990 ◽  
Vol 108 (Supplement) ◽  
pp. 73-79
Author(s):  
Julie A. DeLoia ◽  
Davor Solter
Keyword(s):  
Mouse Genome ◽  
Insertion Site ◽  
Parental Origin ◽  
Incomplete Penetrance ◽  
Endogenous Gene ◽  
Functional Differences ◽  
Genetic Imprinting ◽  
Pronuclear Transfer ◽  
Parent Of Origin ◽  
Transgene Insertion

Genetic imprinting in mice results in functional differences in the oocyte and spermatocyte genomes, as evidenced by both genetic and pronuclear transfer experiments. To gain insights into the molecular mechansims involved in the imprinting process, researchers have studied methylation phenotypes and expression of hemizygous transgenes associated with parental origin. In this report, we describe a transgenic mouse lineage in which expression of both the transgene and an endogenous gene at the insertion site are determined by the parent of origin. The mutation caused by transgene insertion shows variable expressivity and incomplete penetrance in addition to a modified dominant pattern of inheritance.

scholarly journals Differential gene expression in blastocysts following pronuclear transfer

2016 ◽  
Author(s):  
Edward H Morrow ◽  
Fiona C Ingleby
Keyword(s):  
Gene Expression ◽  
Nuclear Transfer ◽  
Expression Profiles ◽  
Conclusive Evidence ◽  
Developmental Pathways ◽  
Primary Concern ◽  
Safety And Efficacy ◽  
Differential Gene ◽  
Pronuclear Transfer ◽  
Cell Expression

Nuclear transfer techniques (a.k.a. mitochondrial replacement therapies) are currently under development to provide a route to eliminating particular instances of mitochondrial disease from the germline. Before these kinds of techniques are implemented clinically it is of primary concern that their safety and efficacy is established. In a recent paper, Hyslop et al (2016) utilized a specific version of pronuclear transfer (PNT) to investigate the consequences for gene expression in the developing embryo, which may indicate whether or not developmental pathways have been perturbed. However, the study was only able to include a small number of blastocysts within each treatment group, although a larger number of single cell expression profiles from each blastocyst were acquired. Using simulated datasets we show that the size and experimental design of this study cannot provide conclusive evidence that expression profiles of manipulated or control samples are indistinguishable from one another due to low power.

scholarly journals Erratum: Corrigendum: Towards clinical application of pronuclear transfer to prevent mitochondrial DNA disease

Nature ◽  
2016 ◽  
Vol 538 (7626) ◽  
pp. 542-542
Author(s):  
Louise A. Hyslop ◽  
Paul Blakeley ◽  
Lyndsey Craven ◽  
Jessica Richardson ◽  
Norah M. E. Fogarty ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Clinical Application ◽  
Pronuclear Transfer
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