A novel bioactive peptide derived from enzymatic hydrolysis of Ruditapes philippinarum: Purification and investigation of its free-radical quenching potential

2013 ◽  
Vol 48 (2) ◽  
pp. 325-330 ◽  
Author(s):  
Eun-Kyung Kim ◽  
Jin-Woo Hwang ◽  
Yon-Suk Kim ◽  
Chang-Bum Ahn ◽  
You-Jin Jeon ◽  
...  
Author(s):  
Douglass Taber

Alkenes are usually reduced by catalytic hydrogenation. Diimide reduction is a mild and neutral alternative. Keith R. Buszek, now at the University of Missouri, Kansas City, has shown (J. Org. Chem. 2007, 72, 3125) that the reduction can conveniently be carried out on resin-bound alkenes, using 2-NBSH (o-nitrobenzenesulfonylhydrazide) with Et3 N for convenient room temperature diimide generation. Ozone can be difficult to dispense accurately on small scale. Masahito Ochiai of the University of Tokushima has uncovered (J. Am. Chem. Soc. 2007, 129, 2772) an alternative, using acid-promoted Ph-I=O. Isolated alkenes also work well. MCPBA is the reagent most commonly used for alkene epoxidation. Payne oxidation (H2O2 /CH3CN) is a convenient and inexpensive alternative. In the course of a study of the enantioselective enzymatic hydrolysis of 6, Takeshi Sugai of Keio University has described (Tetrahedron Lett. 2007, 48, 979) a practical procedure for multigram Payne epoxidation of 5. Several procedures have been put forward for functionalizing terminal alkenes, exemplified by 7. Stefan Grimme and Armido Studer of the Universität Münster have developed (J. Am. Chem. Soc. 2007, 129, 4498) a free radical alkene amination, represented by the conversion of 7 to 9. Tehshik P. Yoon of the University of Wisconsin has found (J. Am. Chem. Soc. 2007, 129, 1866) that Cu catalyzes the addition of oxaziridines such as 10 to alkenes, to make 11. Shinji Nakamura of the University of Tokyo and Masanobu Uchiyama of the University of Tokyo and RIKEN have established (J. Am. Chem. Soc. 2007, 129, 28) that the anion from Cu promoted addition of the silyl zinc reagent to alkenes is long-lived enough to be trapped by electrophiles, including H+ to give 12. Hideki Yorimitsu and Koichiro Oshima of Kyoto University have developed (J. Am. Chem. Soc. 2007, 129, 6094) a complementary transformation, Ni-catalyzed addition of 13 to give 14. The conversion of 7 to 15 reported (Organic Lett. 2007, 9, 53) by Li-Biao Han of the National Institute of Advanced Industrial Science and Technology, Tsukuba, is likely also a free-radical process.


2020 ◽  
Vol 11 (4) ◽  
pp. 3539-3548
Author(s):  
Pedro Sousa ◽  
Sandra Borges ◽  
Manuela Pintado

Edible insects are a promising protein source for the future generation, due to their nutritional composition, sustainability and low environmental impact.


Author(s):  
Marcin Lukasiewicz ◽  
Anna Osowiec ◽  
Magdalena Marciniak

2018 ◽  
Author(s):  
Ángel Batallas ◽  
Erenio González ◽  
Carmen Salvador ◽  
Jonathan Villavicencio ◽  
Humberto González Gavilánez ◽  
...  

2019 ◽  
Vol 15 (3) ◽  
pp. 296-303 ◽  
Author(s):  
Swapnil Gaikwad ◽  
Avinash P. Ingle ◽  
Silvio Silverio da Silva ◽  
Mahendra Rai

Background: Enzymatic hydrolysis of cellulose is an expensive approach due to the high cost of an enzyme involved in the process. The goal of the current study was to apply magnetic nanomaterials as a support for immobilization of enzyme, which helps in the repeated use of immobilized enzyme for hydrolysis to make the process cost-effective. In addition, it will also provide stability to enzyme and increase its catalytic activity. Objective: The main aim of the present study is to immobilize cellulase enzyme on Magnetic Nanoparticles (MNPs) in order to enable the enzyme to be re-used for clean sugar production from cellulose. Methods: MNPs were synthesized using chemical precipitation methods and characterized by different techniques. Further, cellulase enzyme was immobilized on MNPs and efficacy of free and immobilized cellulase for hydrolysis of cellulose was evaluated. Results: Enzymatic hydrolysis of cellulose by immobilized enzyme showed enhanced catalytic activity after 48 hours compared to free enzyme. In first cycle of hydrolysis, immobilized enzyme hydrolyzed the cellulose and produced 19.5 ± 0.15 gm/L of glucose after 48 hours. On the contrary, free enzyme produced only 13.7 ± 0.25 gm/L of glucose in 48 hours. Immobilized enzyme maintained its stability and produced 6.15 ± 0.15 and 3.03 ± 0.25 gm/L of glucose in second and third cycle, respectively after 48 hours. Conclusion: This study will be very useful for sugar production because of enzyme binding efficiency and admirable reusability of immobilized enzyme, which leads to the significant increase in production of sugar from cellulosic materials.


2020 ◽  
Vol 204 ◽  
pp. 106407 ◽  
Author(s):  
Shengxin An ◽  
Wenzhi Li ◽  
Fengyang Xue ◽  
Xu Li ◽  
Ying Xia ◽  
...  

2013 ◽  
Vol 85 (17) ◽  
pp. 8121-8126 ◽  
Author(s):  
Britta Opitz ◽  
Andreas Prediger ◽  
Christian Lüder ◽  
Marrit Eckstein ◽  
Lutz Hilterhaus ◽  
...  

2014 ◽  
Vol 98 (12) ◽  
pp. 5765-5774 ◽  
Author(s):  
Yaping Shang ◽  
Rongxin Su ◽  
Renliang Huang ◽  
Yang Yang ◽  
Wei Qi ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document