Chitin and chitosan preparation from shrimp shells using optimized enzymatic deproteinization

2012 ◽  
Vol 47 (12) ◽  
pp. 2032-2039 ◽  
Author(s):  
Islem Younes ◽  
Olfa Ghorbel-Bellaaj ◽  
Rim Nasri ◽  
Moncef Chaabouni ◽  
Marguerite Rinaudo ◽  
...  
Keyword(s):  
Shrimp Shells ◽  
Enzymatic Deproteinization ◽  
Chitin And Chitosan

Characterization of chitin and chitosan extracted from shrimp shells by two methods

e-Polymers ◽  
2010 ◽  
Vol 10 (1) ◽  
Author(s):  
Carmiña Gartner ◽  
Carlos Alberto Peláez ◽  
Betty Lucy López
Keyword(s):  
Low Cost ◽  
Magic Angle Spinning ◽  
Chemical Extraction ◽  
Magic Angle ◽  
X Ray Diffraction ◽  
Shrimp Shells ◽  
Before And After ◽  
Chitin Extraction ◽  
Angle Spinning ◽  
Chitin And Chitosan

AbstractShrimp shells from Penaeus Vannamei species were hydrolyzed for chitin extraction by a chemical and a papain enzymatic method. Composition of shells was analyzed and their microstructure was characterized before and after hydrolysis by microscopy. Chitin fibers arrangement in the tissue was preserved after chemical extraction, but after papain hydrolysis the tissue presented structural disarrangement indicating that papain reacts indistinctly with peptidic and N-acetyl linkages. Although chemical purification is very effective, by-products are not recoverable. Conversely, papain hydrolysis yields partially purified chitosan but permits aminoacids isolation, which is important in food industry. This method has other advantages such as low cost and easy accessibility of papain. Chitin and chitosan were characterized by thermogravimetric analysis, infrared spectrophotometry and capillary electrophoresis. Degree of N-acetylation (DA) was determined by cross-polarization magic angle spinning nuclear magnetic resonance (CPMAS 13CNMR) or potentiometry and crystallinity was measured by X ray diffraction.

Improved method for production of chitin and chitosan from shrimp shells

2020 ◽  
Vol 489 ◽  
pp. 107913 ◽  
Author(s):  
Trang Si Trung ◽  
Le Huyen Tram ◽  
Nguyen Van Tan ◽  
Nguyen Van Hoa ◽  
Nguyen Cong Minh ◽  
...  
Keyword(s):  
Improved Method ◽  
Shrimp Shells ◽  
Chitin And Chitosan

scholarly journals Chitin and Chitosans: Characteristics, Eco-Friendly Processes, and Applications in Cosmetic Science

Marine Drugs ◽  
2019 ◽  
Vol 17 (6) ◽  
pp. 369 ◽  
Author(s):  
Cristina Casadidio ◽  
Dolores Vargas Peregrina ◽  
Maria Rosa Gigliobianco ◽  
Siyuan Deng ◽  
Roberta Censi ◽  
...  
Keyword(s):  
Oral Care ◽  
Biological Properties ◽  
Pharmaceutical Products ◽  
Renewable Source ◽  
Prevention And Treatment ◽  
Enzymatic Deproteinization ◽  
Physicochemical And Biological Properties ◽  
Chitin And Chitosan

Huge amounts of chitin and chitosans can be found in the biosphere as important constituents of the exoskeleton of many organisms and as waste by worldwide seafood companies. Presently, politicians, environmentalists, and industrialists encourage the use of these marine polysaccharides as a renewable source developed by alternative eco-friendly processes, especially in the production of regular cosmetics. The aim of this review is to outline the physicochemical and biological properties and the different bioextraction methods of chitin and chitosan sources, focusing on enzymatic deproteinization, bacteria fermentation, and enzymatic deacetylation methods. Thanks to their biodegradability, non-toxicity, biocompatibility, and bioactivity, the applications of these marine polymers are widely used in the contemporary manufacturing of biomedical and pharmaceutical products. In the end, advanced cosmetics based on chitin and chitosans are presented, analyzing different therapeutic aspects regarding skin, hair, nail, and oral care. The innovative formulations described can be considered excellent candidates for the prevention and treatment of several diseases associated with different body anatomical sectors.

Production of high-quality chitin and chitosan from preconditioned shrimp shells

2006 ◽  
Vol 81 (7) ◽  
pp. 1113-1118 ◽  
Author(s):  
Nguyen Van Toan ◽  
Chuen-How Ng ◽  
Kyaw Nyein Aye ◽  
Trung Si Trang ◽  
Willem F. Stevens
Keyword(s):  
High Quality ◽  
Shrimp Shells ◽  
Chitin And Chitosan

Extraction and Characterization of Chitin and Chitosan Biopolymer as Wound Healing Material Using Shrimp Shells

2021 ◽  
Vol 10 (2) ◽  
pp. 69-75
Author(s):  
Mavis Ampah ◽  
Margaret Akyea Brago ◽  
Mercy Adusei Boatemaa ◽  
Paul Arthur ◽  
Maxwell Mamfe Sakyiamah
Keyword(s):  
Wound Healing ◽  
Antimicrobial Property ◽  
Industrial Applications ◽  
Chemical Extraction ◽  
Morbidity Rate ◽  
Mortality And Morbidity ◽  
Antimicrobial Studies ◽  
Shrimp Shells ◽  
Specific Temperature ◽  
Chitin And Chitosan

Wound refers to any damage or disruption to the normal anatomical structure. In our everyday pathology, wounds remains one of the demanding clinical problems with it related complications which increases mortality and morbidity rate yearly. In this study, chitin and chitosan were extracted from shrimp shells waste by chemical method, treated with an acid and an alkali which gave a yield of 12 % and 66.57 % respectively. The chemical extraction method included demineralization where the sample was treated with hydrochloric acid (HCL) under a specific temperature. The sample was then treated with sodium hydroxide (NaOH) to remove all the protein in the material at a specific temperature. To obtain the chitosan, the chitin was treated with 50% NaOH at a temperature of 1000C. E.coli proved to be more susceptible in antimicrobial studies with a value of 11.67± 0.47. The FTIR spectra gave a characteristic bands of –NH at 3430.09 cm-1, OH at 3256.32 cm-1.At 2960.75 cm-1, NH was attached to a single bond. The characteristics of produced chitosan were in accordance with the commercial standard that showed a higher percentile yield posing many properties of commercial value and greater scope of industrial applications. This study revealed that shrimp shell waste could be effectively utilized for the extraction of chitin, chitosan, and chitooligomer for industrial applications. The zone of inhibition study of E.coli shows that chitosan and COS may have a high antimicrobial property hence it usefulness in the wound healing management.

scholarly journals Genetic Improvement of Bacillus licheniformis Strains for Efficient Deproteinization of Shrimp Shells and Production of High-Molecular-Mass Chitin and Chitosan

2010 ◽  
Vol 76 (24) ◽  
pp. 8211-8221 ◽  
Author(s):  
Kerstin Hoffmann ◽  
Gabriele Daum ◽  
Marina Köster ◽  
Werner-Michael Kulicke ◽  
Heike Meyer-Rammes ◽  
...  
Keyword(s):  
Bacillus Licheniformis ◽  
Chitinase Activity ◽  
Size Exclusion ◽  
Resonance Spectroscopy ◽  
Phenotypic Properties ◽  
Double Deletion ◽  
Shrimp Shells ◽  
Proteolytic Activities ◽  
Chitin And Chitosan ◽  
Long Chain

ABSTRACT By targeted deletion of the polyglutamate operon (pga) in Bacillus licheniformis F11, a derivative form, F11.1 (Δpga), was obtained that, along with lacking polyglutamate (PGA) formation, displayed enhanced proteolytic activities. The phenotypic properties were maintained in a strain in which the chiBA operon was additionally deleted: F11.4 (ΔchiBA Δpga). These genetically modified strains, carrying the Δpga deletion either alone (F11.1) or together with the ΔchiBA (F11.4) deletion, were used in fermentations (20-liter scale) aiming at the deproteinization of shrimp shells in order to obtain long-chain chitin. After chemical deacetylation, the resulting chitosan samples were analyzed by nuclear magnetic resonance spectroscopy, size exclusion chromatography, and viscometry and compared to a chitosan preparation that was produced in parallel by chemical methods by a commercial chitosan supplier (GSRmbH). Though faint lipid impurities were present in the fermented polysaccharides, the viscosity of the material produced with the double-deletion mutant F11.4 (Δpga ΔchiBA) was higher than that of the chemically produced and commercially available samples (Cognis GmbH). Thus, enhanced proteolytic activities and a lack of chitinase activity render the double mutant F11.4 a powerful tool for the production of long-chain chitosan.

scholarly journals Shrimp shells, Chitin and chitosan powders effect on growth of Lycopersicon esculentum and their ability to induce resistance against Fusarium oxysporum f.sp. radicis-lycopersici attack

2018 ◽  
Author(s):  
Rkhaila Amine ◽  
Ounine Khadija
Keyword(s):  
Lycopersicon Esculentum ◽  
Fusarium Oxysporum ◽  
Dry Weight ◽  
Germination Percentage ◽  
Shrimp Shells ◽  
Induce Resistance ◽  
Chitin And Chitosan ◽  
Germination Parameters

The potential of co-products of shrimp shells on the germination parameters, growth, and for inducing defense reactions in tomato (Lycopersicon esculentum) plants inoculated with Fusarium oxysporum f.sp. radicis-lycopersici (FORL) was investigated in vitro and in vivo.It was found that chitosan at 50mg/l increased the germination percentage by 2.66%; the shrimp shells powder at 50mg/l was best in increasing the length of radical and length and weight of hypocotyl in vitro. In Vivo, chitosan at 50mg/l increased length, fresh/dry weight of aerial and radicle parts compared to control. An inhibition of FORL growth in vitro was obtained by addition of chitosan at 100mg/l. In addition, chitin at 25 mg/l decreased the foliar alteration index by 73.34% compared to control.

Chemical extraction and modification of chitin and chitosan from shrimp shells

2021 ◽  
Vol 159 ◽  
pp. 110709
Author(s):  
Majid Pakizeh ◽  
Ali Moradi ◽  
Toktam Ghassemi
Keyword(s):  
Chemical Extraction ◽  
Shrimp Shells ◽  
Chitin And Chitosan
Sign in / Sign up

Export Citation Format

Share Document