One-step simultaneous purification of three water-soluble constituents in crude extracts from Danshen by adsorption chromatography on oligo-β-cyclodextrin substituted agarose gel media

2007 ◽  
Vol 42 (3) ◽  
pp. 480-485 ◽  
Author(s):  
Jun Xu ◽  
Tianwei Tan ◽  
Jan-Christer Janson
Keyword(s):  
Water Soluble ◽  
Adsorption Chromatography ◽  
Agarose Gel ◽  
Crude Extracts ◽  
One Step

scholarly journals Separation and purification of the alkaline phosphatase and a phosphodiesterase from Halobacterium cutirubrum

1979 ◽  
Vol 181 (2) ◽  
pp. 347-353 ◽  
Author(s):  
P S Fitt ◽  
P Baddoo
Keyword(s):  
Alkaline Phosphatase ◽  
Gel Filtration ◽  
Adsorption Chromatography ◽  
Agarose Gel ◽  
Good Recovery ◽  
Separation And Purification ◽  
Phosphodiesterase Activity ◽  
No Adsorption ◽  
Molecular Weights ◽  
Crude Extracts

1. Halobacterium cutirubrum alkaline phosphatase is associated in crude extracts with a phosphodiesterase. 2. The enzymes were stabilized in buffers containing both (NH4)2SO4 and 10 mM-Mn2+. 3. Adsorption chromatography on Sepharose 6B/agarose-gel columns in the presence of 1.4M-(NH4)2SO4 gave a phosphatase-free phosphodiesterase and the alkaline phosphatase associated with some phosphodiesterase activity. 4. Further chromatography of the separated enzymes gave a good recovery of greater than 600-fold purified phosphodiesterase and greater than 3000-fold purified alkaline phosphatase. 5. The requirements of these enzymes and their relationship to each other was examined. 6. A detailed study showed that the alkaline phosphatase was adsorbed at least partially to agarose and dextran columns at all (NH4)2SO4 concentrations from 0.25 to 2M. 7. In contrast, no adsorption of the enzyme or protein standards was evident in 2.5M-KCl/l M-NaCl or 0.25 M-KCl/0.1 M-NaCl, in agreement with previous studies by Louis, Peterkin & Fitt [(1971) Biochem. J. 121, 635-641], thus confirming the validity of gel filtration in 2.5 M-KCl/1 M-NaCl as a method for determining the approximate molecular weights of extremehalophile proteins.

One-step, ultrasonication-mobilized, solvent-free extraction/synthesis of nanocurcumin from turmeric

RSC Advances ◽  
2015 ◽  
Vol 5 (60) ◽  
pp. 48391-48398 ◽  
Author(s):  
Judy Gopal ◽  
Manikandan Muthu ◽  
Se-Chul Chun
Keyword(s):  
Solvent Free ◽  
Water Soluble ◽  
One Step

One step ultrasonication for direct recovery of water soluble nanocurcumin from turmeric.

scholarly journals Facile one-step synthesis of quaternary AgInZnS quantum dots and their applications for causing bioeffects and detecting Cu2+

RSC Advances ◽  
2020 ◽  
Vol 10 (16) ◽  
pp. 9172-9181 ◽  
Author(s):  
Xiao-Le Han ◽  
Qingyu Li ◽  
Hao Hao ◽  
Chenyin Liu ◽  
Run Li ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Optical Properties ◽  
Hydrothermal Reaction ◽  
Water Soluble ◽  
Step Method ◽  
One Step

Water-soluble AgInZnS quantum dots were synthesized with glutathione as a stabilizer by a facile one-step method based on a hydrothermal reaction at 110 °C. It exhibited excellent optical properties, which can be used as sensor to detect Cu2+.

DNA–EB in agarose gel assay: a simple methodology in the search for DNA-binders in crude extracts from actinomycetes

2016 ◽  
Vol 8 (12) ◽  
pp. 2653-2659
Author(s):  
Anelize Bauermeister ◽  
Munira Muhammad Abdel Baqui ◽  
Luiz Alberto Beraldo Moraes
Keyword(s):  
Agarose Gel ◽  
Screening Process ◽  
Replication Studies ◽  
Crude Extracts ◽  
Dna Binders

Here, a methodology was proposed to detect DNA-binders in a screening process with crude extracts produced by actinomycetes, positive extracts were subjected to replication studies by LC-MS/MS.

Water-soluble hyperbranched polyglycerol photosensitizer for enhanced photodynamic therapy

2020 ◽  
Vol 11 (23) ◽  
pp. 3913-3921
Author(s):  
Bowen Xiang ◽  
Yudong Xue ◽  
Zhiyong Liu ◽  
Jia Tian ◽  
Holger Frey ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Water Soluble ◽  
One Step ◽  
New Type ◽  
Hyperbranched Polyglycerol

Herein, we successfully fabricated a new type of water-soluble, hyperbranched polyglycerol photosensitizer through one-step esterification between water-soluble hyperbranched polyglycerol (hbPG) and fluorophenylporphyrin (FP).

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