One-step separation and purification of hydrolysable tannins from Geranium wilfordii Maxim by adsorption chromatography on cross-linked 12% agarose gel

2011 ◽  
Vol 34 (9) ◽  
pp. 995-998 ◽  
Author(s):  
Dan Liu ◽  
Yan Ma ◽  
Ye Wang ◽  
Zhiguo Su ◽  
Ming Gu ◽  
...  
Keyword(s):  
Adsorption Chromatography ◽  
Agarose Gel ◽  
Separation And Purification ◽  
Hydrolysable Tannins ◽  
One Step

scholarly journals Separation and purification of the alkaline phosphatase and a phosphodiesterase from Halobacterium cutirubrum

1979 ◽  
Vol 181 (2) ◽  
pp. 347-353 ◽  
Author(s):  
P S Fitt ◽  
P Baddoo
Keyword(s):  
Alkaline Phosphatase ◽  
Gel Filtration ◽  
Adsorption Chromatography ◽  
Agarose Gel ◽  
Good Recovery ◽  
Separation And Purification ◽  
Phosphodiesterase Activity ◽  
No Adsorption ◽  
Molecular Weights ◽  
Crude Extracts

1. Halobacterium cutirubrum alkaline phosphatase is associated in crude extracts with a phosphodiesterase. 2. The enzymes were stabilized in buffers containing both (NH4)2SO4 and 10 mM-Mn2+. 3. Adsorption chromatography on Sepharose 6B/agarose-gel columns in the presence of 1.4M-(NH4)2SO4 gave a phosphatase-free phosphodiesterase and the alkaline phosphatase associated with some phosphodiesterase activity. 4. Further chromatography of the separated enzymes gave a good recovery of greater than 600-fold purified phosphodiesterase and greater than 3000-fold purified alkaline phosphatase. 5. The requirements of these enzymes and their relationship to each other was examined. 6. A detailed study showed that the alkaline phosphatase was adsorbed at least partially to agarose and dextran columns at all (NH4)2SO4 concentrations from 0.25 to 2M. 7. In contrast, no adsorption of the enzyme or protein standards was evident in 2.5M-KCl/l M-NaCl or 0.25 M-KCl/0.1 M-NaCl, in agreement with previous studies by Louis, Peterkin & Fitt [(1971) Biochem. J. 121, 635-641], thus confirming the validity of gel filtration in 2.5 M-KCl/1 M-NaCl as a method for determining the approximate molecular weights of extremehalophile proteins.

ChemInform Abstract: Fast One-Step Separation and Purification of Buckminsterfullerene, C60, from Carbon Soot.

ChemInform ◽  
2010 ◽  
Vol 23 (45) ◽  
pp. no-no
Author(s):  
K. CHATTERJEE ◽  
D. H. PARKER ◽  
P. WURZ ◽  
K. R. LYKKE ◽  
D. M. GRUEN ◽  
...  
Keyword(s):  
Separation And Purification ◽  
Carbon Soot ◽  
One Step ◽  
Buckminsterfullerene C60

One-step chromatography method for efficient separation and purification of R-phycoerythrin from Polysiphonia urceolata

2005 ◽  
Vol 116 (1) ◽  
pp. 91-100 ◽  
Author(s):  
Lu-Ning Liu ◽  
Xiu-Lan Chen ◽  
Xi-Ying Zhang ◽  
Yu-Zhong Zhang ◽  
Bai-Cheng Zhou
Keyword(s):  
Separation And Purification ◽  
Chromatography Method ◽  
Efficient Separation ◽  
One Step
Sign in / Sign up

Export Citation Format

Share Document