DNA–EB in agarose gel assay: a simple methodology in the search for DNA-binders in crude extracts from actinomycetes

2016 ◽  
Vol 8 (12) ◽  
pp. 2653-2659
Author(s):  
Anelize Bauermeister ◽  
Munira Muhammad Abdel Baqui ◽  
Luiz Alberto Beraldo Moraes
Keyword(s):  
Agarose Gel ◽  
Screening Process ◽  
Replication Studies ◽  
Crude Extracts ◽  
Dna Binders

Here, a methodology was proposed to detect DNA-binders in a screening process with crude extracts produced by actinomycetes, positive extracts were subjected to replication studies by LC-MS/MS.

scholarly journals Separation and purification of the alkaline phosphatase and a phosphodiesterase from Halobacterium cutirubrum

1979 ◽  
Vol 181 (2) ◽  
pp. 347-353 ◽  
Author(s):  
P S Fitt ◽  
P Baddoo
Keyword(s):  
Alkaline Phosphatase ◽  
Gel Filtration ◽  
Adsorption Chromatography ◽  
Agarose Gel ◽  
Good Recovery ◽  
Separation And Purification ◽  
Phosphodiesterase Activity ◽  
No Adsorption ◽  
Molecular Weights ◽  
Crude Extracts

1. Halobacterium cutirubrum alkaline phosphatase is associated in crude extracts with a phosphodiesterase. 2. The enzymes were stabilized in buffers containing both (NH4)2SO4 and 10 mM-Mn2+. 3. Adsorption chromatography on Sepharose 6B/agarose-gel columns in the presence of 1.4M-(NH4)2SO4 gave a phosphatase-free phosphodiesterase and the alkaline phosphatase associated with some phosphodiesterase activity. 4. Further chromatography of the separated enzymes gave a good recovery of greater than 600-fold purified phosphodiesterase and greater than 3000-fold purified alkaline phosphatase. 5. The requirements of these enzymes and their relationship to each other was examined. 6. A detailed study showed that the alkaline phosphatase was adsorbed at least partially to agarose and dextran columns at all (NH4)2SO4 concentrations from 0.25 to 2M. 7. In contrast, no adsorption of the enzyme or protein standards was evident in 2.5M-KCl/l M-NaCl or 0.25 M-KCl/0.1 M-NaCl, in agreement with previous studies by Louis, Peterkin & Fitt [(1971) Biochem. J. 121, 635-641], thus confirming the validity of gel filtration in 2.5 M-KCl/1 M-NaCl as a method for determining the approximate molecular weights of extremehalophile proteins.

Holding replication studies to mainstream standards of evidence

2018 ◽  
Vol 41 ◽  
Author(s):  
Duane T. Wegener ◽  
Leandre R. Fabrigar
Keyword(s):  
Replication Studies ◽  
Replication Research ◽  
Standards Of Evidence ◽  
Do So

AbstractReplications can make theoretical contributions, but are unlikely to do so if their findings are open to multiple interpretations (especially violations of psychometric invariance). Thus, just as studies demonstrating novel effects are often expected to empirically evaluate competing explanations, replications should be held to similar standards. Unfortunately, this is rarely done, thereby undermining the value of replication research.

Interdisciplinary Therapy Assessments for the Older Adult

2012 ◽  
Vol 17 (1) ◽  
pp. 11-16
Author(s):  
Lynn Chatfield ◽  
Sandra Christos ◽  
Michael McGregor
Keyword(s):  
Older Adults ◽  
Health Care ◽  
Standardized Testing ◽  
Health Care Providers ◽  
Outcome Measurement ◽  
Care Providers ◽  
Patient Centered ◽  
Screening Process ◽  
Evidenced Based ◽  
Changing Economy

In a changing economy and a changing industry, health care providers need to complete thorough, comprehensive, and efficient assessments that provide both an accurate depiction of the patient's deficits and a blueprint to the path of treatment for older adults. Through standardized testing and observations as well as the goals and evidenced-based treatment plans we have devised, health care providers can maximize outcomes and the functional levels of patients. In this article, we review an interdisciplinary assessment that involves speech-language pathology, occupational therapy, physical therapy, and respiratory therapy to work with older adults in health care settings. Using the approach, we will examine the benefits of collaboration between disciplines, an interdisciplinary screening process, and the importance of sharing information from comprehensive discipline-specific evaluations. We also will discuss the importance of having an understanding of the varied scopes of practice, the utilization of outcome measurement tools, and a patient-centered assessment approach to care.

Replication of Experiments Evaluating Impact of Psychological Distance on Moral Judgment

2014 ◽  
Vol 45 (3) ◽  
pp. 223-231 ◽  
Author(s):  
Iris L. Žeželj ◽  
Biljana R. Jokić
Keyword(s):  
Moral Judgment ◽  
Social Distance ◽  
Psychological Distance ◽  
Temporal Distance ◽  
Construal Level ◽  
Systematic Effect ◽  
Replication Studies ◽  
Opposite Conclusion ◽  
Moral Transgressions ◽  
Replication Of Experiments

Eyal, Liberman, and Trope (2008) established that people judged moral transgressions more harshly and virtuous acts more positively when the acts were psychologically distant than close. In a series of conceptual and direct replications, Gong and Medin (2012) came to the opposite conclusion. Attempting to resolve these inconsistencies, we conducted four high-powered replication studies in which we varied temporal distance (Studies 1 and 3), social distance (Study 2) or construal level (Study 4), and registered their impact on moral judgment. We found no systematic effect of temporal distance, the effect of social distance consistent with Eyal et al., and the reversed effect of direct construal level manipulation, consistent with Gong and Medin. Possible explanations for the incompatible results are discussed.

Phytochemical characterization of antimycobacterial crude extracts from medicinal plants traditionally used in Mozambique

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
X Luo ◽  
D Pires ◽  
JA Aínsa ◽  
B Gracia ◽  
S Mulhovo ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Crude Extracts
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