Development of an in vitro confinement test to predict the clinical handling of polymer-based injectable bone substitutes

2013 ◽  
Vol 32 (8) ◽  
pp. 1379-1384 ◽  
Author(s):  
Matilde Bongio ◽  
M. Reza Nejadnik ◽  
F. Kurtis Kasper ◽  
Antonios G. Mikos ◽  
John A. Jansen ◽  
...  
Keyword(s):  
Bone Substitutes ◽  
Confinement Test

scholarly journals Effect of Gold Nanoparticles and Silicon on the Bioactivity and Antibacterial Properties of Hydroxyapatite/Chitosan/Tricalcium Phosphate-Based Biomicroconcretes

Materials ◽  
2021 ◽  
Vol 14 (14) ◽  
pp. 3854
Author(s):  
Joanna Czechowska ◽  
Ewelina Cichoń ◽  
Anna Belcarz ◽  
Anna Ślósarczyk ◽  
Aneta Zima
Keyword(s):  
Gold Nanoparticles ◽  
Antibacterial Activity ◽  
Tricalcium Phosphate ◽  
Setting Time ◽  
Antibacterial Properties ◽  
Bone Substitutes ◽  
Bacterial Strains ◽  
Setting Times ◽  
Biological Studies

Bioactive, chemically bonded bone substitutes with antibacterial properties are highly recommended for medical applications. In this study, biomicroconcretes, composed of silicon modified (Si-αTCP) or non-modified α-tricalcium phosphate (αTCP), as well as hybrid hydroxyapatite/chitosan granules non-modified and modified with gold nanoparticles (AuNPs), were designed. The developed biomicroconcretes were supposed to combine the dual functions of antibacterial activity and bone defect repair. The chemical and phase composition, microstructure, setting times, mechanical strength, and in vitro bioactive potential of the composites were examined. Furthermore, on the basis of the American Association of Textile Chemists and Colorists test (AATCC 100), adapted for chemically bonded materials, the antibacterial activity of the biomicroconcretes against S. epidermidis, E. coli, and S. aureus was evaluated. All biomicroconcretes were surgically handy and revealed good adhesion between the hybrid granules and calcium phosphate-based matrix. Furthermore, they possessed acceptable setting times and mechanical properties. It has been stated that materials containing AuNPs set faster and possess a slightly higher compressive strength (3.4 ± 0.7 MPa). The modification of αTCP with silicon led to a favorable decrease of the final setting time to 10 min. Furthermore, it has been shown that materials modified with AuNPs and silicon possessed an enhanced bioactivity. The antibacterial properties of all of the developed biomicroconcretes against the tested bacterial strains due to the presence of both chitosan and Au were confirmed. The material modified simultaneously with AuNPs and silicon seems to be the most promising candidate for further biological studies.

scholarly journals Three-Dimensional Bone Substitutes for Oral and Maxillofacial Surgery: Biological and Structural Characterization

2018 ◽  
Vol 9 (4) ◽  
pp. 62 ◽  
Author(s):  
Gianluca Turco ◽  
Davide Porrelli ◽  
Eleonora Marsich ◽  
Federica Vecchies ◽  
Teresa Lombardi ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Clinical Performance ◽  
Maxillofacial Surgery ◽  
Chemical Characterization ◽  
Bone Substitutes ◽  
Human Cell Line ◽  
Structural Features ◽  
Oral And Maxillofacial Surgery ◽  
Cell Adhesion And Proliferation

Background: Bone substitutes, either from human (autografts and allografts) or animal (xenografts) sources, suffer from inherent drawbacks including limited availability or potential infectivity to name a few. In the last decade, synthetic biomaterials have emerged as a valid alternative for biomedical applications in the field of orthopedic and maxillofacial surgery. In particular, phosphate-based bone substitution materials have exhibited a high biocompatibility due to their chemical similitude with natural hydroxyapatite. Besides the nature of the biomaterial, its porous and interconnected architecture is essential for a correct osseointegration. This performance could be predicted with an extensive characterization of the biomaterial in vitro. Methods: In this study, we compared the biological, chemical, and structural features of four different commercially available bone substitutes derived from an animal or a synthetic source. To this end, µ-CT and SEM were used to describe the biomaterials structure. Both FTIR and EDS analyses were carried out to provide a chemical characterization. The results obtained by these techniques were correlated with cell adhesion and proliferation of the osteosarcoma MG-63 human cell line cultured in vitro. Results: The findings reported in this paper indicate a significant influence of both the nature and the structure of the biomaterials in cell adhesion and proliferation, which ultimately could affect the clinical performance of the biomaterials. Conclusions: The four commercially available bone substitutes investigated in this work significantly differed in terms of structural features, which ultimately influenced in vitro cell proliferation and may so affect the clinical performance of the biomaterials.

scholarly journals Drug Leaching Properties of Vancomycin Loaded Mesoporous Hydroxyapatite as Bone Substitutes

Processes ◽  
2019 ◽  
Vol 7 (11) ◽  
pp. 826 ◽  
Author(s):  
Jayasingh Anita Lett ◽  
Suresh Sagadevan ◽  
Joseph Joyce Prabhakar ◽  
Nor Aliya Hamizi ◽  
Irfan Anjum Badruddin ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Pore Size ◽  
In Vitro Release ◽  
Phosphate Buffer Solution ◽  
Bone Substitutes ◽  
Organic Modifiers ◽  
Buffer Solution ◽  
Modern Health Care ◽  
Impregnation Technique

Infections after bone reconstructive surgery become an authentic therapeutic and economic issue when it comes to a modern health care system. In general; infected bone defects are regarded as contraindications for bone grafting. Since the pathogens develop a biofilm on the inner surface of the bone; local delivery of antibiotics becomes more important. The present work focuses on the synthesis of Mesoporous Hydroxyapatite (MPHAP) loaded with drug Vancomycin (Van) and to investigate its loading and leaching ability in phosphate buffer solution (PBS), to be used for post-operative infections. The effect of pore size on MPHAP was analyzed using different fatty acids as organic modifiers. The impacts of various fatty acids chain length on the morphology and pore size were studied. A simple impregnation technique with optimized conditions ensured a high antibiotic loading (up to 0.476 + 0.0135 mg/mL), with a complete in vitro release obtained within 50 h.

A comparison of Thai silk fibroin-based and chitosan-based materials on in vitro biocompatibility for bone substitutes

2009 ◽  
Vol 45 (5) ◽  
pp. 470-477 ◽  
Author(s):  
Nuttapon Vachiraroj ◽  
Juthamas Ratanavaraporn ◽  
Siriporn Damrongsakkul ◽  
Rath Pichyangkura ◽  
Tanom Banaprasert ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Bone Substitutes ◽  
In Vitro Biocompatibility

Apatite Deposition on Organic-Inorganic Hybrids Synthesized from Poly(Vinyl Alcohol) and Various Metal Oxides

2005 ◽  
Vol 284-286 ◽  
pp. 469-472 ◽  
Author(s):  
Caihong You ◽  
Toshiki Miyazaki ◽  
Eiichi Ishida ◽  
Masahiro Ashizuka ◽  
Chikara Ohtsuki ◽  
...  
Keyword(s):  
Metal Oxides ◽  
Vinyl Alcohol ◽  
Calcium Salt ◽  
Mechanical Performance ◽  
Bone Substitutes ◽  
Poly Vinyl Alcohol ◽  
Apatite Formation ◽  
Molybdenum Oxides ◽  
Apatite Deposition

Organic polymers with ability of apatite formation in body environment are expected as novel bone substitutes having not only bone-bonding ability, i.e. bioactivity, but also mechanical performance analogous to natural bone. Several metal oxides have been found to be effective for the apatite deposition in body environment. In addition, release of calcium ions from the materials significantly enhances it. In this study, we attempted to synthesize bioactive organic-inorganic hybrids from Poly(vinyl alcohol) (PVA) by incorporation of various metal oxides and calcium salt. Silica and molybdenum oxides were selected as metal oxides. Ability of apatite formation on the hybrids was examined in vitro using simulated body fluid (SBF, Kokubo solution). Apatite deposition were observed to occur on the surfaces of PVA/silica and PVA/molybdenum oxide hybrids in SBF, when their compositions were appropriately controlled.

scholarly journals Cell Colonization Ability of a Commercialized Large Porous Alveolar Scaffold

2017 ◽  
Vol 2017 ◽  
pp. 1-10
Author(s):  
S. Lemonnier ◽  
T. Bouderlique ◽  
S. Naili ◽  
H. Rouard ◽  
J. Courty ◽  
...  
Keyword(s):  
Bone Substitutes ◽  
Physical Parameters ◽  
Porous Scaffolds ◽  
Cellular Viability ◽  
Negative Effect ◽  
Porous Microstructure ◽  
Colonization Ability ◽  
Large Bone

The use of filling biomaterials or tissue-engineered large bone implant-coupling biocompatible materials and human bone marrow mesenchymal stromal cells seems to be a promising approach to treat critical-sized bone defects. However, the cellular seeding onto and into large porous scaffolds still remains challenging since this process highly depends on the porous microstructure. Indeed, the cells may mainly colonize the periphery of the scaffold, leaving its volume almost free of cells. In this study, we carry out an in vitro study to analyze the ability of a commercialized scaffold to be in vivo colonized by cells. We investigate the influence of various physical parameters on the seeding efficiency of a perfusion seeding protocol using large manufactured bone substitutes. The present study shows that the velocity of the perfusion fluid and the initial cell density seem to impact the seeding results and to have a negative effect on the cellular viability, whereas the duration of the fluid perfusion and the nature of the flow (steady versus pulsed) did not show any influence on either the fraction of seeded cells or the cellular viability rate. However, the cellular repartition after seeding remains highly heterogeneous.

scholarly journals In vitro gentamicin release from commercially available calcium-phosphate bone substitutes influence of carrier type on duration of the release profile

2006 ◽  
Vol 7 (1) ◽  
Author(s):  
Hein P Stallmann ◽  
Chris Faber ◽  
Antonius LJJ Bronckers ◽  
Arie V Nieuw Amerongen ◽  
Paul IJM Wuisman
Keyword(s):  
Calcium Phosphate ◽  
Bone Substitutes ◽  
Release Profile

3D Microtomography Characterization of Dental Implantology Bone Substitutes Used In Vivo

2013 ◽  
Vol 541 ◽  
pp. 97-113 ◽  
Author(s):  
Rossella Bedini ◽  
Deborah Meleo ◽  
Raffaella Pecci
Keyword(s):  
Bone Substitute ◽  
Histological Analysis ◽  
Research Work ◽  
Bone Substitutes ◽  
Human Bone ◽  
Histological Techniques ◽  
In Vitro Characterization

After a short introduction to bone substitute biomaterials and X-ray microtomography, this article describes a research work carried out for in-vitro characterization of bone substitute biomaterials as well as for in-vivo investigation of human bone grafted with biomaterials. Three different bone substitute biomaterials have been analyzed in-vitro by means of 3D microtomographic technique, while human bone samples grafted with bone substitute biomaterials are investigated by 3D microtomography and histological techniques. 3D images of bone substitutes and human bone samples with biomaterials have been obtained, together with morphometric parameters, by microtomography . 2D histological images have also been obtained by traditional technique only for human bone samples with biomaterials. Compared to traditional histological analysis, 3D microtomography shows better results for investigating bone tissue and bone substitute biomaterial, and in a short time. Nevertheless, histological analysis remains the best technique for the observation of soft tissue and blood vessels.

scholarly journals Characterization of Naturally Occurring Bioactive Factor Mixtures for Bone Regeneration

2020 ◽  
Vol 21 (4) ◽  
pp. 1412 ◽  
Author(s):  
Henriette Bretschneider ◽  
Mandy Quade ◽  
Anja Lode ◽  
Michael Gelinsky ◽  
Stefan Rammelt ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Adipose Tissue ◽  
Conditioned Medium ◽  
Platelet Rich Plasma ◽  
Umbilical Vein ◽  
Bone Substitutes ◽  
Angiogenic Potential ◽  
Angiogenic Proteins ◽  
Human Telomerase

In this study, the bone-regenerative potential of bioactive factors derived from adipose tissue, platelet-rich plasma (PRP) and conditioned medium from hypoxia-treated human telomerase immortalized bone-marrow-derived mesenchymal stem cells (hTERT-MSC) was investigated in vitro with the aim to develop cost-effective and efficient bone substitutes for optimized regeneration of bone defects. Adipose tissue was harvested from human donors undergoing reconstructive surgery, and adipose tissue extract (ATE) was prepared. Platelet lysates (PL) were produced by repeated freeze-thaw cycles of PRP, and hypoxia-conditioned medium (HCM) was obtained by culturing human telomerase immortalized bone-marrow-derived mesenchymal stromal cells for 5 days with 1% O2. Besides analysis by cytokine and angiogenesis arrays, ELISA was performed. Angiogenic potential was investigated in cocultures of bone-marrow-derived (BM)-MSC and human umbilical vein endothelial cells. Multiple angiogenic proteins and cytokines were detected in all growth factor mixtures. HCM and ATE contained high amounts of angiogenin and CCL2/MCP-1, whereas PL contained high amounts of IGFBP-1. Culturing cells with HCM and ATE significantly increased specific ALP activity of BM-MSC as well as tubule length and junctions of endothelial networks, indicating osteogenic and angiogenic stimulation. To achieve a synergism between chemoattractive potential and osteogenic and angiogenic differentiation capacity, a combination of different growth factors appears promising for potential clinical applications.

Sign in / Sign up

Export Citation Format

Share Document