Evaluation of bioceramic bone substitutes – hydroxyapatite (HAP), tricalcium phosphate (TCP) and biphasic ceramic (HAP/TCP) in vitro and in vivo

2011 ◽  
Vol 40 (10) ◽  
pp. 1216 ◽  
Author(s):  
I. Salma ◽  
G. Salms ◽  
M. Pilmane ◽  
D. Loca ◽  
J. Locs ◽  
...  
Keyword(s):  
Tricalcium Phosphate ◽  
Bone Substitutes ◽  
Biphasic Ceramic

Preparation of Porous Biphasic Tricalcium Phosphate and Its In Vivo Behavior

2005 ◽  
Vol 284-286 ◽  
pp. 281-284 ◽  
Author(s):  
Masanobu Kamitakahara ◽  
Chikara Ohtsuki ◽  
M. Oishi ◽  
Shinichi Ogata ◽  
Toshiki Miyazaki ◽  
...  
Keyword(s):  
Tricalcium Phosphate ◽  
Ceramic Body ◽  
Potato Starch ◽  
Bone Substitutes ◽  
The Body ◽  
In Vivo Experiments ◽  
Tcp Phase ◽  
Biphasic Ceramic ◽  
Mg Content

Biphasic ceramic consisting of tricalcium phosphate with α- and β -phases (αβ-TCP) is a candidate as biodegradable bone substitutes since its biodegradability may be controlled by the ratio of the phases. In the present study, preparation of porous αβ-TCP body with continuous pores of 10-50 µm in diameter was attempted using additives of Mg, and its in vivo behavior was examined. Powder of β-TCP was mixed with Mg and potato starch to form slurry, followed by loading in polyurethane foam. The sample was fired at 1400°C for 12 hours for sintering process. α-TCP content of the sample decreased with increasing the Mg content, while β-TCP increased. Ceramic body consisting of β-TCP phase was obtained when 1.0 mass% of Mg was added. Porosity of the body decreased with increasing the content of Mg. The αβ-TCP body with 80% porosity was obtained when the content of Mg was 0.1 mass%. The in vivo experiments showed that the rate of degradation of the obtained αβ-TCP was almost same as α-TCP, and much higher than β-TCP.

scholarly journals Antibiotic loaded β-tricalcium phosphate/calcium sulfate for antimicrobial potency, prevention and killing efficacy of Pseudomonas aeruginosa and Staphylococcus aureus biofilms

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Nan Jiang ◽  
Devendra H. Dusane ◽  
Jacob R. Brooks ◽  
Craig P. Delury ◽  
Sean S. Aiken ◽  
...  
Keyword(s):  
Tricalcium Phosphate ◽  
Calcium Sulfate ◽  
Bone Graft Substitute ◽  
In Vitro Assays ◽  
In Vivo Experiments ◽  
Orthopaedic Infections ◽  
Clinical Investigations ◽  
Antimicrobial Potency

AbstractThis study investigated the efficacy of a biphasic synthetic β-tricalcium phosphate/calcium sulfate (β-TCP/CS) bone graft substitute for compatibility with vancomycin (V) in combination with tobramycin (T) or gentamicin (G) evidenced by the duration of potency and the prevention and killing efficacies of P. aeruginosa (PAO1) and S. aureus (SAP231) biofilms in in vitro assays. Antibiotic loaded β-TCP/CS beads were compared with antibiotic loaded beads formed from a well characterized synthetic calcium sulfate (CS) bone void filler. β-TCP/CS antibiotic loaded showed antimicrobial potency against PAO1 in a repeated Kirby-Bauer like zone of inhibition assay for 6 days compared to 8 days for CS. However, both bead types showed potency against SAP231 for 40 days. Both formulations loaded with V + T completely prevented biofilm formation (CFU below detection limits) for the 3 days of the experiment with daily fresh inoculum challenges (P < 0.001). In addition, both antibiotic loaded materials and antibiotic combinations significantly reduced the bioburden of pre-grown biofilms by between 3 and 5 logs (P < 0.001) with V + G performing slightly better against PAO1 than V + T. Our data, combined with previous data on osteogenesis suggest that antibiotic loaded β-TCP/CS may have potential to stimulate osteogenesis through acting as a scaffold as well as simultaneously protecting against biofilm infection. Future in vivo experiments and clinical investigations are warranted to more comprehensively evaluate the use of β-TCP/CS in the management of orthopaedic infections.

scholarly journals Effect of Gold Nanoparticles and Silicon on the Bioactivity and Antibacterial Properties of Hydroxyapatite/Chitosan/Tricalcium Phosphate-Based Biomicroconcretes

Materials ◽  
2021 ◽  
Vol 14 (14) ◽  
pp. 3854
Author(s):  
Joanna Czechowska ◽  
Ewelina Cichoń ◽  
Anna Belcarz ◽  
Anna Ślósarczyk ◽  
Aneta Zima
Keyword(s):  
Gold Nanoparticles ◽  
Antibacterial Activity ◽  
Tricalcium Phosphate ◽  
Setting Time ◽  
Antibacterial Properties ◽  
Bone Substitutes ◽  
Bacterial Strains ◽  
Setting Times ◽  
Biological Studies

Bioactive, chemically bonded bone substitutes with antibacterial properties are highly recommended for medical applications. In this study, biomicroconcretes, composed of silicon modified (Si-αTCP) or non-modified α-tricalcium phosphate (αTCP), as well as hybrid hydroxyapatite/chitosan granules non-modified and modified with gold nanoparticles (AuNPs), were designed. The developed biomicroconcretes were supposed to combine the dual functions of antibacterial activity and bone defect repair. The chemical and phase composition, microstructure, setting times, mechanical strength, and in vitro bioactive potential of the composites were examined. Furthermore, on the basis of the American Association of Textile Chemists and Colorists test (AATCC 100), adapted for chemically bonded materials, the antibacterial activity of the biomicroconcretes against S. epidermidis, E. coli, and S. aureus was evaluated. All biomicroconcretes were surgically handy and revealed good adhesion between the hybrid granules and calcium phosphate-based matrix. Furthermore, they possessed acceptable setting times and mechanical properties. It has been stated that materials containing AuNPs set faster and possess a slightly higher compressive strength (3.4 ± 0.7 MPa). The modification of αTCP with silicon led to a favorable decrease of the final setting time to 10 min. Furthermore, it has been shown that materials modified with AuNPs and silicon possessed an enhanced bioactivity. The antibacterial properties of all of the developed biomicroconcretes against the tested bacterial strains due to the presence of both chitosan and Au were confirmed. The material modified simultaneously with AuNPs and silicon seems to be the most promising candidate for further biological studies.

scholarly journals Supplementation with 45S5 Bioactive Glass Reduces In Vivo Resorption of the β-Tricalcium-Phosphate-Based Bone Substitute Material Vitoss

2019 ◽  
Vol 20 (17) ◽  
pp. 4253 ◽  
Author(s):  
Fabian Westhauser ◽  
Christopher Essers ◽  
Maria Karadjian ◽  
Bruno Reible ◽  
Gerhard Schmidmaier ◽  
...  
Keyword(s):  
Bioactive Glass ◽  
Tricalcium Phosphate ◽  
Bone Substitute ◽  
Degradation Kinetics ◽  
Bone Substitutes ◽  
Micro Computed Tomography ◽  
Immunodeficient Mice ◽  
Bone Substitute Materials ◽  
Osseous Regeneration

Compared to other materials such as 45S5 bioactive glass (BG), β-tricalcium phosphate (β-TCP)-based bone substitutes such as Vitoss show limited material-driven stimulation of osteogenesis and/or angiogenesis. The unfavorable degradation kinetics of β-TCP-based bone substitutes may result in an imbalance between resorption and osseous regeneration. Composite materials like Vitoss BA (Vitoss supplemented with 20 wt % 45S5-BG particles) might help to overcome these limitations. However, the influence of BG particles in Vitoss BA compared to unsupplemented Vitoss on osteogenesis, resorption behavior, and angiogenesis is not yet described. In this study, Vitoss and Vitoss BA scaffolds were seeded with human mesenchymal stromal cells before subcutaneous implantation in immunodeficient mice for 10 weeks. Scaffold resorption was monitored by micro-computed tomography, while osteoid formation and vascularization were assessed by histomorphometry and gene expression analysis. Whilst slightly more osteoid and improved angiogenesis were found in Vitoss BA, maturation of the osteoid was more advanced in Vitoss scaffolds. The volume of Vitoss implants decreased significantly, combined with a significantly increased presence of resorbing cells, whilst the volume remained stable in Vitoss BA scaffolds. Future studies should evaluate the interaction of 45S5-BG with resorbing cells and bone precursor cells in greater detail to improve the understanding and application of β-TCP/45S5-BG composite bone substitute materials.

The effects of Mn and/or Ni dopants on the in vitro/in vivo performance, structural and magnetic properties of β-tricalcium phosphate bioceramics

2019 ◽  
Vol 45 (17) ◽  
pp. 22752-22758 ◽  
Author(s):  
Tankut Ates ◽  
Sergey V. Dorozhkin ◽  
Omer Kaygili ◽  
Mustafa Kom ◽  
Ismail Ercan ◽  
...  
Keyword(s):  
Magnetic Properties ◽  
Tricalcium Phosphate ◽  
Structural And Magnetic Properties

New Composite Material: PLLA and Tricalcium Phosphate for Orthopaedic Applications-In Vitro and In Vivo Studies (Part 1)

2016 ◽  
pp. 173-180
Author(s):  
Jean Charles Le Huec ◽  
Antonio Faundez ◽  
Stephane Aunoble ◽  
Rachid Sadikki ◽  
Julien Rigal
Keyword(s):  
Composite Material ◽  
Tricalcium Phosphate ◽  
In Vivo Studies

Improvement in osseointegration of tricalcium phosphate-zircon for orthopedic applications: an in vitro and in vivo evaluation

2020 ◽  
Vol 58 (8) ◽  
pp. 1681-1693
Author(s):  
Abolfazl Bagherifard ◽  
Hamed Joneidi Yekta ◽  
Hossein Akbari Aghdam ◽  
Mehdi Motififard ◽  
Ehsan Sanatizadeh ◽  
...  
Keyword(s):  
Tricalcium Phosphate ◽  
In Vivo Evaluation ◽  
Orthopedic Applications
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