Pain behaviour assessments by gait and weight bearing in surgically induced osteoarthritis and inflammatory arthritis

2020 ◽  
Vol 225 ◽  
pp. 113079
Author(s):  
Kristina Ängeby Möller ◽  
Cecilia Aulin ◽  
Azar Baharpoor ◽  
Camilla I Svensson
Keyword(s):  
Inflammatory Arthritis ◽  
Weight Bearing ◽  
Pain Behaviour ◽  
Surgically Induced

Conservative management of osteoarthrosis

1973 ◽  
Vol 11 (8) ◽  
pp. 29-30
Keyword(s):  
Articular Cartilage ◽  
Rheumatoid Factor ◽  
Conservative Management ◽  
Inflammatory Arthritis ◽  
Morning Stiffness ◽  
Weight Bearing ◽  
Radiological Evidence ◽  
Wear And Tear ◽  
Joint Erosions ◽  
Synovial Effusion

Osteoarthrosis (osteoarthritis) can be regarded as excessive joint wear and tear to the point where this becomes painful or produces stiffness. It is most disabling in weight-bearing joints, in which wearing away of articular cartilage is accompanied by underlying bony overgrowth and accumulation of a synovial effusion. The mechanism of the pain is uncertain: capsular strains and deep bone sensation may contribute. Diagnosis requires the exclusion of inflammatory arthritis such as rheumatoid; pointers to the latter are morning stiffness, raised ESR, positive tests for rheumatoid factor in the serum and radiological evidence of joint erosions.

scholarly journals Comparison of LABORAS with static incapacitance testing for assessing spontaneous pain behaviour in surgically-induced murine osteoarthritis

2020 ◽  
Vol 2 (4) ◽  
pp. 100101
Author(s):  
Isabell S. von Loga ◽  
Jadwiga Miotla-Zarebska ◽  
Yi-Shu Huang ◽  
Richard Williams ◽  
Luke Jostins ◽  
...  
Keyword(s):  
Spontaneous Pain ◽  
Pain Behaviour ◽  
Surgically Induced

scholarly journals Blocking the tropomyosin receptor kinase A (TrkA) receptor inhibits pain behaviour in two rat models of osteoarthritis

2015 ◽  
Vol 75 (6) ◽  
pp. 1246-1254 ◽  
Author(s):  
Lilian N Nwosu ◽  
Paul I Mapp ◽  
Victoria Chapman ◽  
David A Walsh
Keyword(s):  
Weight Bearing ◽  
Treatment Discontinuation ◽  
Receptor Kinase ◽  
Treatment Protocols ◽  
Pain Behaviour ◽  
Rat Models ◽  
Withdrawal Threshold ◽  
Knee Oa ◽  
Monosodium Iodoacetate ◽  
Kinase A

ObjectivesTropomyosin receptor kinase A (TrkA) mediates nociceptor sensitisation by nerve growth factor (NGF), but it is unknown whether selective TrkA inhibition will be an effective strategy for treating osteoarthritis (OA) pain. We determined the effects of a TrkA inhibitor (AR786) on pain behaviour, synovitis and joint pathology in two rat OA models.MethodsKnee OA was induced in rats by intra-articular monosodium-iodoacetate (MIA) injection or meniscal transection (MNX) and compared with saline-injected or sham-operated controls. Pain behaviour was assessed as weight-bearing asymmetry and paw withdrawal threshold to punctate stimulation. Oral doses (30 mg/kg) of AR786 or vehicle were administered twice daily in either preventive (day −1 to –27) or treatment (day 14–28) protocols. Effect maintenance was evaluated for 2 weeks after treatment discontinuation. Alterations in knee structure (cartilage, subchondral bone and synovium) were examined by macroscopic visualisation of articular surfaces and histopathology.ResultsPreventive AR786 treatment inhibited pain behaviour development and therapeutic treatment attenuated established pain behaviour. Weight-bearing asymmetry increased 1 week after treatment discontinuation, but remained less than in vehicle-treated arthritic rats, whereas paw withdrawal thresholds returned to levels of untreated rats within 5 days of treatment discontinuation. AR786 treatment reduced MIA-induced synovitis and did not significantly affect osteochondral pathology in either model.ConclusionsBlocking NGF activity by inhibiting TrkA reduced pain behaviour in two rat models of OA. Analgesia was observed both using preventive and treatment protocols, and was sustained after treatment discontinuation. Selective inhibitors of TrkA therefore hold potential for OA pain relief.

scholarly journals Chitosan-Hyaluronate Hybrid Gel Intraarticular Injection Delays Osteoarthritis Progression and Reduces Pain in a Rat Meniscectomy Model as Compared to Saline and Hyaluronate Treatment

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Shachar Patchornik ◽  
Edward Ram ◽  
Noah Ben Shalom ◽  
Zvi Nevo ◽  
Dror Robinson
Keyword(s):  
Femoral Condyle ◽  
Weight Bearing ◽  
Cyst Formation ◽  
Hybrid Gel ◽  
Ameliorative Effect ◽  
Control Knee ◽  
Osteoarthritis Progression ◽  
Surgically Induced ◽  
Therapeutic Substance ◽  
Delay Progression

Chitosan-Hyaluronate hybrid gel (CHHG) is a self-forming thermo-responsive hydrogel. The current study was undertaken in order to assess the effect of CHHG on rat's surgically induced osteoarthritis.Methods. Thirteen rats were included in the study. In all rats weight-bearing was assessed using a Linton Incapacitance tester. All rats underwent bilateral medial partial meniscectomy. Four rats received a saline injection in the control knee and a 200-microliter injection of CHHG in the experimental knee. Five rats received a high-molecular weight hyaluronate injection to the control knee and a 200-microliter injection of CHHG in the experimental knee. Four rats underwent the same surgical procedure, allowed to recuperate for seven days and then CHHG and hyaluronate were injected. The animals were followed for 6 weeks. Two weeks after injection of a therapeutic substance the amount of weight-bearing on each knee was evaluated using a Linton Incapacitance meter.Results. Two weeks after induction of osteoarthritis there is less pain in the CHHG-treated knee than in the control-treated knee, as determined using a Lintron Incapacitance meter. After six-weeks the histological appearance of the CHHG-treated knee was superior to that of the controls. This is indicated by thicker cartilage remaining on the medial femoral condyle as well as less cyst formation in the CHHG-treated knee.Discussion. CHHG appears to delay progression of osteoarthritis and lessen pain in a rat surgically-induced knee osteoarthritis model. These results support other published results, indicating that there is an ameliorative effect of chitosan on human and rabbit osteoarthritis.

scholarly journals Shea Nut Oil Extracts Enhance the Intra-Articular Sodium Hyaluronate Effectiveness on Surgically Induced OA Progression in Rats

Nutrients ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 957
Author(s):  
Ing-Jung Chen ◽  
Chih-Shung Wong
Keyword(s):  
Cruciate Ligament ◽  
Weight Bearing ◽  
Symptomatic Relief ◽  
Cartilage Damage ◽  
Joint Damage ◽  
Sodium Hyaluronate ◽  
Cartilage Degeneration ◽  
Knee Oa ◽  
Medial Meniscectomy ◽  
Surgically Induced

Osteoarthritis (OA) progression is associated with joint pain and stiffness. Intra-articular hyaluronic acid (IAHA) injection in knee OA restores the viscoelasticity of the joint and prevents cartilage damage. Shea nut oil extract (SNO) was shown to provide chondroprotection on surgically-induced OA progression in rats. Here we aim to examine IAHA injection supplemented with SNO diet for a synergetic evaluation on the disease progression in OA rats. We employed an anterior cruciate ligament transection plus medial meniscectomy-induced knee OA rat model with up to 12 weeks of sign/behavior observation (knee width, weight-bearing) and histological assessments of joint damage. We found both IAHA and SNO alone significantly attenuated histological changes of cartilage degeneration and synovial reactions in these knee OA rats. Nonetheless, oral SNO alone mitigated OA pain and inflammation while IAHA alone had no significant impact on the weight-bearing test and knee joint swelling. Moreover, with IAHA-treated rats fed with oral SNO diet, additional anti-inflammatory and anti-nociceptive effects were found, which further enhanced and maintained IAHA protection. Given the differential phenotype of oral SNO vs. IAHA, a regimen of IAHA coupled with SNO supplement provides a long-term effect of IAHA treatment. Taken together, the SNO supplement can be safely used as an adjuvant diet for chronic symptomatic relief of OA coupled with IAHA management.

scholarly journals Increased function of pronociceptive TRPV1 at the level of the joint in a rat model of osteoarthritis pain

2013 ◽  
Vol 74 (1) ◽  
pp. 252-259 ◽  
Author(s):  
S Kelly ◽  
R J Chapman ◽  
S Woodhams ◽  
D R Sagar ◽  
J Turner ◽  
...  
Keyword(s):  
Body Temperature ◽  
Rat Model ◽  
Transient Receptor Potential ◽  
Core Body Temperature ◽  
Weight Bearing ◽  
Transient Receptor Potential Vanilloid ◽  
Pain Behaviour ◽  
Therapeutic Benefits ◽  
Trpv1 Receptors ◽  
Core Body

ObjectivesBlockade of transient receptor potential vanilloid 1 (TRPV1) with systemic antagonists attenuates osteoarthritis (OA) pain behaviour in rat models, but on-target-mediated hyperthermia has halted clinical trials. The present study investigated the potential for targeting TRPV1 receptors within the OA joint in order to produce analgesia.MethodsThe presence of TRPV1 receptors in human synovium was detected using western blotting and immunohistochemistry. In a rat model of OA, joint levels of an endogenous ligand for TRPV1, 12-hydroxy-eicosatetraenoic acid (12-HETE), were quantified using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Effects of peripheral administration of the TRPV1 receptor antagonist JNJ-17203212 on afferent fibre activity, pain behaviour and core body temperature were investigated. Effects of a spinal administration of JNJ-17203212 on dorsal horn neuronal responses were studied.ResultsWe demonstrate increased TRPV1 immunoreactivity in human OA synovium, confirming the diseased joint as a potential therapeutic target for TRPV1-mediated analgesia. In a model of OA pain, we report increased joint levels of 12-HETE, and the sensitisation of joint afferent neurones to mechanical stimulation of the knee. Local administration of JNJ-17203212 reversed this sensitisation of joint afferents and inhibited pain behaviour (weight-bearing asymmetry), to a comparable extent as systemic JNJ-17203212, in this model of OA pain, but did not alter core body temperature. There was no evidence for increased TRPV1 function in the spinal cord in this model of OA pain.ConclusionsOur data provide a clinical and mechanistic rationale for the future investigation of the therapeutic benefits of intra-articular administration of TRPV1 antagonists for the treatment of OA pain.

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