High throughput dual-wavelength temperature distribution imaging via compressive imaging

2018 ◽  
Vol 410 ◽  
pp. 287-291 ◽  
Author(s):  
Xu-Ri Yao ◽  
Ruo-Ming Lan ◽  
Xue-Feng Liu ◽  
Ge Zhu ◽  
Fu Zheng ◽  
...  
Keyword(s):  
Temperature Distribution ◽  
High Throughput ◽  
Dual Wavelength ◽  
Compressive Imaging

A Thermal System for a High Throughput Continuous Flow Polymerase Chain Reaction Device (CFPCR)

2008 ◽  
Author(s):  
P.-C. Chen ◽  
D. S. Park ◽  
B. H. You ◽  
N. Kim ◽  
T. Park ◽  
...  
Keyword(s):  
Temperature Distribution ◽  
High Throughput ◽  
Continuous Flow ◽  
Thermal Environment ◽  
Infrared Camera ◽  
Thermal System ◽  
Uniform Temperature ◽  
Chain Reaction ◽  
Copper Strip ◽  
Polymerase Chain

A thermal system used to evaluate a high throughput 96 continuous flow polymerase chain reactor (CFPCR) array was designed, fabricated, and tested. Each polymerase chain reactor (PCR) in the array required three different temperature zones to realize denaturaiton at 90°C–94°C, renaturation at 50°C–70°C, and extension at 72°C; a total of 288 temperature zones were required for the 96 CFPCR array. In an initial configuration, 18 copper strips were used to define the 288 temperature zones. Each copper strip was controlled by a PID feedback control loop. Numerical simulations were used to understand the thermal crosstalk phenomena between the micromilled copper strips, which were tightly packed since the high throughput micro-titer plate format restricted each CFPCR to a square 8 mm on a side. The lowest achievable temperature in each renaturation zone in this complicated thermal environment was also identified. Thermal crosstalk limited the minimum renaturation temperature to 61.1°C. An infrared camera was used to investigate the temperature uniformity over a 0.25 mm thick polycarbonate sheet mounted on the thermal system. The temperature distribution was not uniform due to poor contact between the copper strips and device, warm air accumulated between the packed copper strips, and greater heat transfer around the boundaries of the device. More work is required to overcome these limitations and achieve a more uniform temperature distribution for a multi well CFPCR.

scholarly journals Freeze-Float System for High-throughput Measurement of Ice Nucleation

2021 ◽  
Author(s):  
Yuki Kamijo ◽  
Ratmir Derda
Keyword(s):  
Temperature Distribution ◽  
Sample Size ◽  
High Throughput ◽  
High Throughput Screening ◽  
Cooling System ◽  
Ice Nucleation ◽  
Uniform Temperature ◽  
Detection Program ◽  
Accuracy Of Measurement ◽  
Platform System

In this publication, we developed the high throughput screening implementation of freeze-float selection platform system we established in the previous publication. The goal of this publication is to expand the system to higher throughput and accuracy. In the following sections, we describe the steps for automated droplets generations, adaptation of freeze-float selection using controlled and uniform temperature cooling system, and semi-automated droplets detection program. We aimed to improve previously published system to add functional advantages, such as; a) increased efficiency of the screening with fewer manipulation steps; b) increased accuracy of measurement due to the increased sample size; c) increased uniformity of temperature distribution by incorporating the controlled-rate freezer. In the following sections, we describe the steps for automated droplets generations, freeze-float system adaptations using controlled and uniform temperature cooling system, and semi-automated droplets detection program.

A Dual Wavelength Polymerization and Bioconjugation Strategy for High Throughput Synthesis of Multivalent Ligands

2019 ◽  
Vol 141 (50) ◽  
pp. 19823-19830 ◽  
Author(s):  
Zihao Li ◽  
Shashank Kosuri ◽  
Henry Foster ◽  
Jarrod Cohen ◽  
Coline Jumeaux ◽  
...  
Keyword(s):  
High Throughput ◽  
Dual Wavelength ◽  
Multivalent Ligands

scholarly journals High-throughput label-free flow cytometry based on matched-filter compressive imaging

2018 ◽  
Vol 9 (12) ◽  
pp. 6145
Author(s):  
Cong Ba ◽  
William J. Shain ◽  
Thomas G. Bifano ◽  
Jerome Mertz
Keyword(s):  
Flow Cytometry ◽  
High Throughput ◽  
Matched Filter ◽  
Free Flow ◽  
Label Free ◽  
Compressive Imaging

154: Integration of TPSA and High-Throughput Mass Spectrometry Data Improves Prostate Cancer Prediction

2007 ◽  
Vol 177 (4S) ◽  
pp. 52-53
Author(s):  
Stefano Ongarello ◽  
Eberhard Steiner ◽  
Regina Achleitner ◽  
Isabel Feuerstein ◽  
Birgit Stenzel ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Mass Spectrometry ◽  
High Throughput ◽  
Mass Spectrometry Data ◽  
Cancer Prediction
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