scholarly journals High-throughput label-free flow cytometry based on matched-filter compressive imaging

2018 ◽  
Vol 9 (12) ◽  
pp. 6145
Author(s):  
Cong Ba ◽  
William J. Shain ◽  
Thomas G. Bifano ◽  
Jerome Mertz
Keyword(s):  
Flow Cytometry ◽  
High Throughput ◽  
Matched Filter ◽  
Free Flow ◽  
Label Free ◽  
Compressive Imaging

A high-throughput flow cytometry-on-a-CMOS platform for single-cell dielectric spectroscopy at microwave frequencies

Lab on a Chip ◽  
2018 ◽  
Vol 18 (14) ◽  
pp. 2065-2076 ◽  
Author(s):  
Jun-Chau Chien ◽  
Ali Ameri ◽  
Erh-Chia Yeh ◽  
Alison N. Killilea ◽  
Mekhail Anwar ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Dielectric Properties ◽  
Single Cell ◽  
High Throughput ◽  
Dielectric Spectroscopy ◽  
Free Flow ◽  
Label Free ◽  
Microwave Frequencies

This work presents a microfluidics-integrated label-free flow cytometry-on-a-CMOS platform for the characterization of the cytoplasm dielectric properties at microwave frequencies.

Application of machine learning-driven label-free flow cytometry to analyze T cell products

Cytotherapy ◽  
2020 ◽  
Vol 22 (5) ◽  
pp. S132-S133
Author(s):  
H. Ochiai ◽  
K. Teranishi ◽  
K. Toda ◽  
K. Sugimoto ◽  
S. Ota
Keyword(s):  
Machine Learning ◽  
Flow Cytometry ◽  
T Cell ◽  
Free Flow ◽  
Label Free

scholarly journals Label Free Identification of Peripheral Blood Eosinophils Using High-Throughput Imaging Flow Cytometry

2017 ◽  
Vol 139 (2) ◽  
pp. AB163
Author(s):  
Justyna Piasecka ◽  
Holger Hennig ◽  
Fabian J. Theis ◽  
Paul Rees ◽  
Huw D. Summers ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
High Throughput ◽  
Peripheral Blood ◽  
Label Free ◽  
Imaging Flow Cytometry ◽  
Blood Eosinophils ◽  
High Throughput Imaging

Label-free flow cytometry using multiplex coherent anti-Stokes Raman scattering (MCARS) for the analysis of biological specimens

2011 ◽  
Vol 36 (12) ◽  
pp. 2309 ◽  
Author(s):  
Charles H. Camp, Jr. ◽  
Siva Yegnanarayanan ◽  
Ali A. Eftekhar ◽  
Ali Adibi
Keyword(s):  
Flow Cytometry ◽  
Raman Scattering ◽  
Free Flow ◽  
Label Free ◽  
Anti Stokes

Label‐Free Flow Cytometry Analysis of Subvisible Aggregates in Liquid IgG1 Antibody Formulations

2014 ◽  
Vol 103 (1) ◽  
pp. 90-99 ◽  
Author(s):  
Hirotaka Nishi ◽  
Roman Mathäs ◽  
Robert Fürst ◽  
Gerhard Winter
Keyword(s):  
Flow Cytometry ◽  
Free Flow ◽  
Label Free ◽  
Flow Cytometry Analysis ◽  
Igg1 Antibody

scholarly journals High-throughput label-free molecular fingerprinting flow cytometry

2019 ◽  
Vol 5 (1) ◽  
pp. eaau0241 ◽  
Author(s):  
Kotaro Hiramatsu ◽  
Takuro Ideguchi ◽  
Yusuke Yonamine ◽  
SangWook Lee ◽  
Yizhi Luo ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Single Cell ◽  
High Throughput ◽  
Single Cell Analysis ◽  
Single Cells ◽  
Fluorescent Labeling ◽  
Live Cells ◽  
Molecular Fingerprinting ◽  
Label Free ◽  
Indispensable Tool

Flow cytometry is an indispensable tool in biology for counting and analyzing single cells in large heterogeneous populations. However, it predominantly relies on fluorescent labeling to differentiate cells and, hence, comes with several fundamental drawbacks. Here, we present a high-throughput Raman flow cytometer on a microfluidic chip that chemically probes single live cells in a label-free manner. It is based on a rapid-scan Fourier-transform coherent anti-Stokes Raman scattering spectrometer as an optical interrogator, enabling us to obtain the broadband molecular vibrational spectrum of every single cell in the fingerprint region (400 to 1600 cm−1) with a record-high throughput of ~2000 events/s. As a practical application of the method not feasible with conventional flow cytometry, we demonstrate high-throughput label-free single-cell analysis of the astaxanthin productivity and photosynthetic dynamics ofHaematococcus lacustris.

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