Live-cell 3D super-resolution imaging in thick biological samples

2011 ◽  
Vol 8 (12) ◽  
pp. 1047-1049 ◽  
Author(s):  
Francesca Cella Zanacchi ◽  
Zeno Lavagnino ◽  
Michela Perrone Donnorso ◽  
Alessio Del Bue ◽  
Laura Furia ◽  
...  
Keyword(s):  
Biological Samples ◽  
Super Resolution ◽  
Live Cell ◽  
Resolution Imaging

Instant Live-Cell Super-Resolution Imaging of Cellular Structures by Nanoinjection of Fluorescent Probes

Nano Letters ◽  
2015 ◽  
Vol 15 (2) ◽  
pp. 1374-1381 ◽  
Author(s):  
Simon Hennig ◽  
Sebastian van de Linde ◽  
Martina Lummer ◽  
Matthias Simonis ◽  
Thomas Huser ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Super Resolution ◽  
Live Cell ◽  
Cellular Structures ◽  
Resolution Imaging

Live-Cell Super-Resolution Imaging with Synthetic Fluorophores

2012 ◽  
Vol 63 (1) ◽  
pp. 519-540 ◽  
Author(s):  
Sebastian van de Linde ◽  
Mike Heilemann ◽  
Markus Sauer
Keyword(s):  
Super Resolution ◽  
Live Cell ◽  
Resolution Imaging

Live cell super resolution imaging by radial fluctuations using fluorogen binding tags

Nanoscale ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 3626-3632 ◽  
Author(s):  
Muthukumaran Venkatachalapathy ◽  
Vivek Belapurkar ◽  
Mini Jose ◽  
Arnaud Gautier ◽  
Deepak Nair
Keyword(s):  
Super Resolution ◽  
Time Lapse ◽  
Live Cell ◽  
Resolution Imaging

Combination of SRRF and stochastic labeling based on FAST:Fluorogen complexes to achieve super-resolution in 2D, 3D and in time-lapse.

scholarly journals FluoSim: simulator of single molecule dynamics for fluorescence live-cell and super-resolution imaging of membrane proteins

2020 ◽  
Author(s):  
Matthieu Lagardère ◽  
Ingrid Chamma ◽  
Emmanuel Bouilhol ◽  
Macha Nikolski ◽  
Olivier Thoumine
Keyword(s):  
Real Time ◽  
Protein Dynamics ◽  
Single Molecule ◽  
Imaging Techniques ◽  
Simulated Data ◽  
Super Resolution ◽  
Molecular Complexes ◽  
Live Cell ◽  
Single Molecule Level ◽  
Resolution Imaging

AbstractFluorescence live-cell and super-resolution microscopy methods have considerably advanced our understanding of the dynamics and mesoscale organization of macro-molecular complexes that drive cellular functions. However, different imaging techniques can provide quite disparate information about protein motion and organization, owing to their respective experimental ranges and limitations. To address these limitations, we present here a unified computer program that allows one to model and predict membrane protein dynamics at the ensemble and single molecule level, so as to reconcile imaging paradigms and quantitatively characterize protein behavior in complex cellular environments. FluoSim is an interactive real-time simulator of protein dynamics for live-cell imaging methods including SPT, FRAP, PAF, and FCS, and super-resolution imaging techniques such as PALM, dSTORM, and uPAINT. The software, thoroughly validated against experimental data on the canonical neurexin-neuroligin adhesion complex, integrates diffusion coefficients, binding rates, and fluorophore photo-physics to calculate in real time the distribution of thousands of independent molecules in 2D cellular geometries, providing simulated data of protein dynamics and localization directly comparable to actual experiments.

scholarly journals A specific hybridisation internalisation probe (SHIP) enables precise live-cell and super-resolution imaging of internalized cargo

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Sara Hernández-Pérez ◽  
Pieta K. Mattila
Keyword(s):  
Super Resolution ◽  
Cell Receptor ◽  
Live Cell ◽  
Receptor Ligand ◽  
Vesicle Traffic ◽  
Specific Hybridization ◽  
Multiple Imaging ◽  
Resolution Imaging ◽  
Membrane Bound ◽  
Quenching Probe

AbstractFacilitated by the advancements in microscopy, our understanding of the complexity of intracellular vesicle traffic has dramatically increased in recent years. However, distinguishing between plasma membrane-bound or internalised ligands remains a major challenge for the studies of cargo sorting to endosomal compartments, especially in small and round cells such as lymphocytes. The specific hybridization internalisation probe (SHIP) assay, developed for flow cytometry studies, employs a ssDNA fluorescence internalisation probe and a complementary ssDNA quenching probe to unambiguously detect the internalized receptors/cargo. Here, we adopted the SHIP assay to study the trafficking of receptor/ligand complexes using B lymphocytes and B cell receptor-mediated antigen internalization as a model system. Our study demonstrates the potential of the SHIP assay for improving the imaging of internalized receptor/ligand complexes and establishes the compatibility of this assay with multiple imaging modalities, including live-cell imaging and super-resolution microscopy.

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