Nitric oxide induces epidermal stem cell de-adhesion by targeting integrin β1 and Talin via the cGMP signalling pathway

Nitric Oxide ◽  
2018 ◽  
Vol 78 ◽  
pp. 1-10 ◽  
Author(s):  
Rixing Zhan ◽  
Fan Wang ◽  
Ying Wu ◽  
Ying Wang ◽  
Wei Qian ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Stem Cell ◽  
Signalling Pathway ◽  
Epidermal Stem Cell ◽  
Integrin Β1 ◽  
Cgmp Signalling

scholarly journals Dopamine in the ink defence system of Sepia officinalis: biosynthesis, vesicular compartmentation in mature ink gland cells, nitric oxide (NO)/cGMP-induced depletion and fate in secreted ink1

2004 ◽  
Vol 378 (3) ◽  
pp. 785-791 ◽  
Author(s):  
Gabriella FIORE ◽  
Annarita POLI ◽  
Anna Di COSMO ◽  
Marco d'ISCHIA ◽  
Anna PALUMBO
Keyword(s):  
Nitric Oxide ◽  
Tyrosine Hydroxylase ◽  
Physical Adsorption ◽  
Signalling Pathway ◽  
Nitric Oxide Donor ◽  
Sepia Officinalis ◽  
Release Process ◽  
No Donor ◽  
Cgmp Signalling ◽  
Incubation Experiments

The biosynthesis, localization and fate of catecholamines in the ink gland of the cuttlefish Sepia officinalis were investigated by combined biochemical and immunohistocytochemical methodologies. HPLC analysis of crude ink gland extracts indicated the presence of dopa (2.18±0.82 nmol/mg of protein) and DA (dopamine, 0.06±0.02 nmol/mg of protein), but no detectable noradrenaline or adrenaline. DA was shown to derive from l-tyrosine, according to experiments performed by incubating intact ink glands with [l-14C]tyrosine. The biosynthetic process involves a tyrosine hydroxylase and a dopa decarboxylase pathway and is independent of tyrosinase. The tyrosine hydroxylase activity was detected under conditions of tyrosinase suppression in the cytosolic fraction, but not in the melanosomal fraction, of ink gland extracts, and the presence of the enzyme was confirmed by Western-blot analysis. Dopa and DA were found to be released from the ink glands by processes controlled through the NMDA-nitric oxide-cGMP (where NMDA stands for N-methyl-d-aspartate) signalling pathway, as apparent from incubation experiments performed with [l-14C]tyrosine in the presence of NMDA, diethylamine NONOate (diethylamine diazeniumdiolate), a nitric oxide donor, 8-bromo-cGMP or a guanylyl cyclase inhibitor. Immunohistochemical results coupled with electron microscopy indicated that DA was concentrated in vesicles specifically localized in the mature melanin-producing cells of the ink gland proximal to the lumen and separated from the melanin-containing melanosomes. NMDA receptor stimulation or exposure to an NO donor caused a marked loss of DA immunoreactivity in mature cells, consistent with a release process. In the lumen of the ink gland, where mature exhausted cells pour their contents, DA immunoreactivity was found to be associated with the melanin granules, due apparently to physical adsorption. Overall, these results point to DA as a marker of cell maturation in Sepia ink gland subject to release by the NO/cGMP signalling pathway, and disclose apparently overlooked DA–melanin interactions in secreted ink of possible relevance to the defence mechanism.

Nitric oxide promotes epidermal stem cell proliferation via FOXG1-c-Myc signalling

Nitric Oxide ◽  
2018 ◽  
Vol 73 ◽  
pp. 1-8 ◽  
Author(s):  
Rixing Zhan ◽  
Fan Wang ◽  
Ying Wu ◽  
Ying Wang ◽  
Wei Qian ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Cell Proliferation ◽  
Stem Cell ◽  
Epidermal Stem Cell ◽  
Stem Cell Proliferation

Ghrelin Induces Growth Hormone Secretion Via a Nitric Oxide /cGMP Signalling Pathway

2008 ◽  
Vol 20 (3) ◽  
pp. 406-412 ◽  
Author(s):  
F. Rodríguez-Pacheco ◽  
R. M. Luque ◽  
M. Tena-Sempere ◽  
M. M. Malagón ◽  
J. P. Castaño
Keyword(s):  
Nitric Oxide ◽  
Growth Hormone ◽  
Hormone Secretion ◽  
Growth Hormone Secretion ◽  
Signalling Pathway ◽  
Cgmp Signalling

scholarly journals Nitric oxide promotes epidermal stem cell migration via cGMP-Rho GTPase signalling

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Rixing Zhan ◽  
Weifeng He ◽  
Fan Wang ◽  
Zhihui Yao ◽  
Jianglin Tan ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Stem Cell ◽  
Cell Migration ◽  
Rho Gtpase ◽  
Epidermal Stem Cell ◽  
Stem Cell Migration

Dual actions of dephostatin on the nitric oxide/cGMP-signalling pathway in porcine iliac arteries

2005 ◽  
Vol 521 (1-3) ◽  
pp. 124-132 ◽  
Author(s):  
Anna Asplund Persson ◽  
Peter Gunnarsson ◽  
Eva Lindström ◽  
Magnus Grenegård
Keyword(s):  
Nitric Oxide ◽  
Signalling Pathway ◽  
Iliac Arteries ◽  
Cgmp Signalling

scholarly journals Physical Exercise and Cardiac Repair: The Potential Role of Nitric Oxide in Boosting Stem Cell Regenerative Biology

Antioxidants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1002
Author(s):  
Fabiola Marino ◽  
Mariangela Scalise ◽  
Eleonora Cianflone ◽  
Luca Salerno ◽  
Donato Cappetta ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Stem Cell ◽  
Physical Exercise ◽  
Cell Biology ◽  
Molecular Mechanisms ◽  
Heart Function ◽  
Stem Cell Biology ◽  
Myocardial Repair ◽  
Beneficial Effects

Over the years strong evidence has been accumulated showing that aerobic physical exercise exerts beneficial effects on the prevention and reduction of cardiovascular risk. Exercise in healthy subjects fosters physiological remodeling of the adult heart. Concurrently, physical training can significantly slow-down or even reverse the maladaptive pathologic cardiac remodeling in cardiac diseases, improving heart function. The underlying cellular and molecular mechanisms of the beneficial effects of physical exercise on the heart are still a subject of intensive study. Aerobic activity increases cardiovascular nitric oxide (NO) released mainly through nitric oxidase synthase 3 activity, promoting endothelium-dependent vasodilation, reducing vascular resistance, and lowering blood pressure. On the reverse, an imbalance between increasing free radical production and decreased NO generation characterizes pathologic remodeling, which has been termed the “nitroso-redox imbalance”. Besides these classical evidence on the role of NO in cardiac physiology and pathology, accumulating data show that NO regulate different aspects of stem cell biology, including survival, proliferation, migration, differentiation, and secretion of pro-regenerative factors. Concurrently, it has been shown that physical exercise generates physiological remodeling while antagonizes pathologic remodeling also by fostering cardiac regeneration, including new cardiomyocyte formation. This review is therefore focused on the possible link between physical exercise, NO, and stem cell biology in the cardiac regenerative/reparative response to physiological or pathological load. Cellular and molecular mechanisms that generate an exercise-induced cardioprotective phenotype are discussed in regards with myocardial repair and regeneration. Aerobic training can benefit cells implicated in cardiovascular homeostasis and response to damage by NO-mediated pathways that protect stem cells in the hostile environment, enhance their activation and differentiation and, in turn, translate to more efficient myocardial tissue regeneration. Moreover, stem cell preconditioning by and/or local potentiation of NO signaling can be envisioned as promising approaches to improve the post-transplantation stem cell survival and the efficacy of cardiac stem cell therapy.

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