Active ingredients of Plastrum Testudinis inhibit epidermal stem cell apoptosis in serum-deprived culture

2011 ◽  
Vol 9 (8) ◽  
pp. 888-893
Author(s):  
L Chen
Keyword(s):  
Stem Cell ◽  
Cell Apoptosis ◽  
Epidermal Stem Cell ◽  
Active Ingredients

scholarly journals Functional Specificity of the Members of the Sos Family of Ras-GEF Activators: Novel Role of Sos2 in Control of Epidermal Stem Cell Homeostasis

Cancers ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 2152
Author(s):  
Fernando C. Baltanás ◽  
Cynthia Mucientes-Valdivieso ◽  
L. Francisco Lorenzo-Martín ◽  
Natalia Fernández-Parejo ◽  
Rósula García-Navas ◽  
...  
Keyword(s):  
Stem Cell ◽  
Layer Structure ◽  
3D Culture ◽  
Hair Follicles ◽  
Specific Cell ◽  
Epidermal Stem Cell ◽  
Primary Keratinocytes ◽  
Cell Homeostasis ◽  
Primary Mouse

Prior reports showed the critical requirement of Sos1 for epithelial carcinogenesis, but the specific functionalities of the homologous Sos1 and Sos2 GEFs in skin homeostasis and tumorigenesis remain unclear. Here, we characterize specific mechanistic roles played by Sos1 or Sos2 in primary mouse keratinocytes (a prevalent skin cell lineage) under different experimental conditions. Functional analyses of actively growing primary keratinocytes of relevant genotypes—WT, Sos1-KO, Sos2-KO, and Sos1/2-DKO—revealed a prevalent role of Sos1 regarding transcriptional regulation and control of RAS activation and mechanistic overlapping of Sos1 and Sos2 regarding cell proliferation and survival, with dominant contribution of Sos1 to the RAS-ERK axis and Sos2 to the RAS-PI3K/AKT axis. Sos1/2-DKO keratinocytes could not grow under 3D culture conditions, but single Sos1-KO and Sos2-KO keratinocytes were able to form pseudoepidermis structures that showed disorganized layer structure, reduced proliferation, and increased apoptosis in comparison with WT 3D cultures. Remarkably, analysis of the skin of both newborn and adult Sos2-KO mice uncovered a significant reduction of the population of stem cells located in hair follicles. These data confirm that Sos1 and Sos2 play specific, cell-autonomous functions in primary keratinocytes and reveal a novel, essential role of Sos2 in control of epidermal stem cell homeostasis.

scholarly journals Reducing alcohol and/or cocaine-induced reward and toxicity via an epidermal stem cell-based gene delivery platform

2021 ◽  
Author(s):  
Qingyao Kong ◽  
Yuanyuan Li ◽  
Jiping Yue ◽  
Xiaoyang Wu ◽  
Ming Xu
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Gene Delivery ◽  
Unmet Need ◽  
Skin Grafts ◽  
Epidermal Stem Cell ◽  
Epidermal Stem Cells ◽  
Concurrent Use ◽  
Delivery Platform ◽  
Glucagon Like Peptide 1

AbstractAlcohol use disorder (AUD) is one of the foremost public health problems. Alcohol is also frequently co-abused with cocaine. There is a huge unmet need for the treatment of AUD and/or cocaine co-abuse. We recently demonstrated that skin grafts generated from mouse epidermal stem cells that had been engineered by CRISPR-mediated genome editing could be transplanted onto mice as a gene delivery platform. Here, we show that expression of the glucagon-like peptide-1 (GLP1) gene delivered by epidermal stem cells attenuated development and reinstatement of alcohol-induced drug-taking and seeking as well as voluntary oral alcohol consumption. GLP1 derived from the skin grafts decreased alcohol-induced increase in dopamine levels in the nucleus accumbens. In exploring the potential of this platform in reducing concurrent use of drugs, we developed a novel co-grafting procedure for both modified human butyrylcholinesterase (hBChE)- and GLP1-expressing cells. Epidermal stem cell-derived hBChE and GLP1 reduced acquisition of drug-taking and toxicity induced by alcohol and cocaine co-administration. These results imply that cutaneous gene delivery through skin transplants may add a new option to treat drug abuse and co-abuse.

M1 macrophage-derived exosomes transfer miR-222 to induce bone marrow mesenchymal stem cell apoptosis

2021 ◽  
Author(s):  
Yanyan Qi ◽  
Tingting Zhu ◽  
Tingting Zhang ◽  
Xi Wang ◽  
Wenbo Li ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Apoptosis ◽  
M1 Macrophage

scholarly journals Complement C5a induces mesenchymal stem cell apoptosis during the progression of chronic diabetic complications

Diabetologia ◽  
2017 ◽  
Vol 60 (9) ◽  
pp. 1822-1833 ◽  
Author(s):  
Ming Zhu ◽  
Xiao He ◽  
Xiao-Hui Wang ◽  
Wei Qiu ◽  
Wei Xing ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Diabetic Complications ◽  
Cell Apoptosis ◽  
Complement C5a

scholarly journals Epigenetic Regulation of Epidermal Stem Cell Biomarkers and Their Role in Wound Healing

2015 ◽  
Vol 17 (1) ◽  
pp. 16 ◽  
Author(s):  
Sabita Saldanha ◽  
Kendra Royston ◽  
Neha Udayakumar ◽  
Trygve Tollefsbol
Keyword(s):  
Wound Healing ◽  
Stem Cell ◽  
Epigenetic Regulation ◽  
Epidermal Stem Cell

Toward Epidermal Stem Cell-Mediated ex Vivo Gene Therapy of Junctional Epidermolysis Bullosa

2000 ◽  
Vol 11 (16) ◽  
pp. 2283-2287 ◽  
Author(s):  
Elena Dellambra ◽  
Graziella Pellegrini ◽  
Liliana Guerra ◽  
Giuliana Ferrari ◽  
Giovanna Zambruno ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Stem Cell ◽  
Epidermolysis Bullosa ◽  
Ex Vivo ◽  
Epidermal Stem Cell ◽  
Junctional Epidermolysis Bullosa ◽  
Ex Vivo Gene Therapy

In vivo recording of epidermal stem cell redox state

2017 ◽  
Vol 108 ◽  
pp. S38
Author(s):  
Alexander Martin Wolf ◽  
Shigeo Ohta
Keyword(s):  
Stem Cell ◽  
Redox State ◽  
Epidermal Stem Cell
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