Epigallocatechin-3-gallate relaxes the isolated bovine ophthalmic artery: Involvement of phosphoinositide 3-kinase-Akt-nitric oxide/cGMP signalling pathway

2009 ◽  
Vol 608 (1-3) ◽  
pp. 48-53 ◽  
Author(s):  
Maria Rosaria Romano ◽  
Marcello Diego Lograno
Keyword(s):  
Nitric Oxide ◽  
Ophthalmic Artery ◽  
Signalling Pathway ◽  
Cgmp Signalling ◽  
Phosphoinositide 3 Kinase

scholarly journals Dopamine in the ink defence system of Sepia officinalis: biosynthesis, vesicular compartmentation in mature ink gland cells, nitric oxide (NO)/cGMP-induced depletion and fate in secreted ink1

2004 ◽  
Vol 378 (3) ◽  
pp. 785-791 ◽  
Author(s):  
Gabriella FIORE ◽  
Annarita POLI ◽  
Anna Di COSMO ◽  
Marco d'ISCHIA ◽  
Anna PALUMBO
Keyword(s):  
Nitric Oxide ◽  
Tyrosine Hydroxylase ◽  
Physical Adsorption ◽  
Signalling Pathway ◽  
Nitric Oxide Donor ◽  
Sepia Officinalis ◽  
Release Process ◽  
No Donor ◽  
Cgmp Signalling ◽  
Incubation Experiments

The biosynthesis, localization and fate of catecholamines in the ink gland of the cuttlefish Sepia officinalis were investigated by combined biochemical and immunohistocytochemical methodologies. HPLC analysis of crude ink gland extracts indicated the presence of dopa (2.18±0.82 nmol/mg of protein) and DA (dopamine, 0.06±0.02 nmol/mg of protein), but no detectable noradrenaline or adrenaline. DA was shown to derive from l-tyrosine, according to experiments performed by incubating intact ink glands with [l-14C]tyrosine. The biosynthetic process involves a tyrosine hydroxylase and a dopa decarboxylase pathway and is independent of tyrosinase. The tyrosine hydroxylase activity was detected under conditions of tyrosinase suppression in the cytosolic fraction, but not in the melanosomal fraction, of ink gland extracts, and the presence of the enzyme was confirmed by Western-blot analysis. Dopa and DA were found to be released from the ink glands by processes controlled through the NMDA-nitric oxide-cGMP (where NMDA stands for N-methyl-d-aspartate) signalling pathway, as apparent from incubation experiments performed with [l-14C]tyrosine in the presence of NMDA, diethylamine NONOate (diethylamine diazeniumdiolate), a nitric oxide donor, 8-bromo-cGMP or a guanylyl cyclase inhibitor. Immunohistochemical results coupled with electron microscopy indicated that DA was concentrated in vesicles specifically localized in the mature melanin-producing cells of the ink gland proximal to the lumen and separated from the melanin-containing melanosomes. NMDA receptor stimulation or exposure to an NO donor caused a marked loss of DA immunoreactivity in mature cells, consistent with a release process. In the lumen of the ink gland, where mature exhausted cells pour their contents, DA immunoreactivity was found to be associated with the melanin granules, due apparently to physical adsorption. Overall, these results point to DA as a marker of cell maturation in Sepia ink gland subject to release by the NO/cGMP signalling pathway, and disclose apparently overlooked DA–melanin interactions in secreted ink of possible relevance to the defence mechanism.

Nitric oxide induces epidermal stem cell de-adhesion by targeting integrin β1 and Talin via the cGMP signalling pathway

Nitric Oxide ◽  
2018 ◽  
Vol 78 ◽  
pp. 1-10 ◽  
Author(s):  
Rixing Zhan ◽  
Fan Wang ◽  
Ying Wu ◽  
Ying Wang ◽  
Wei Qian ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Stem Cell ◽  
Signalling Pathway ◽  
Epidermal Stem Cell ◽  
Integrin Β1 ◽  
Cgmp Signalling

Ghrelin Induces Growth Hormone Secretion Via a Nitric Oxide /cGMP Signalling Pathway

2008 ◽  
Vol 20 (3) ◽  
pp. 406-412 ◽  
Author(s):  
F. Rodríguez-Pacheco ◽  
R. M. Luque ◽  
M. Tena-Sempere ◽  
M. M. Malagón ◽  
J. P. Castaño
Keyword(s):  
Nitric Oxide ◽  
Growth Hormone ◽  
Hormone Secretion ◽  
Growth Hormone Secretion ◽  
Signalling Pathway ◽  
Cgmp Signalling

Dual actions of dephostatin on the nitric oxide/cGMP-signalling pathway in porcine iliac arteries

2005 ◽  
Vol 521 (1-3) ◽  
pp. 124-132 ◽  
Author(s):  
Anna Asplund Persson ◽  
Peter Gunnarsson ◽  
Eva Lindström ◽  
Magnus Grenegård
Keyword(s):  
Nitric Oxide ◽  
Signalling Pathway ◽  
Iliac Arteries ◽  
Cgmp Signalling

The nitric oxide-cyclic GMP signalling pathway in rat brain

1993 ◽  
Vol 32 (11) ◽  
pp. 1267-1277 ◽  
Author(s):  
Eric Southam ◽  
John Garthwaite
Keyword(s):  
Nitric Oxide ◽  
Rat Brain ◽  
Cyclic Gmp ◽  
Signalling Pathway

The Nitric Oxide Signalling Pathway in Aortic Aneurysm and Dissection

2021 ◽  
Author(s):  
Marta Toral ◽  
Andrea Fuente‐Alonso ◽  
Miguel R. Campanero ◽  
Juan Miguel Redondo
Keyword(s):  
Nitric Oxide ◽  
Aortic Aneurysm ◽  
Signalling Pathway ◽  
Aortic Aneurysm And Dissection
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