scholarly journals Preferential binding of positive nanoparticles on cell membranes is due to electrostatic interactions: A too simplistic explanation that does not take into account the nanoparticle protein corona

2017 ◽  
Vol 70 ◽  
pp. 889-896 ◽  
Author(s):  
Valérie Forest ◽  
Jérémie Pourchez
Keyword(s):  
Electrostatic Interactions ◽  
Cell Membranes ◽  
Protein Corona ◽  
Preferential Binding

Penetration and preferential binding of charged nanoparticles to mixed lipid monolayers: interplay of lipid packing and charge density

Soft Matter ◽  
2020 ◽  
Author(s):  
Anurag Chaudhury ◽  
Koushik Debnath ◽  
Wei Bu ◽  
Nikhil R. Jana ◽  
Jaydeep Kumar Basu
Keyword(s):  
Charge Density ◽  
Biomedical Applications ◽  
Cell Membranes ◽  
Lipid Monolayers ◽  
Cytotoxic Effects ◽  
Lipid Packing ◽  
Preferential Binding ◽  
Charged Nanoparticles

Designing of nanoparticles (NPs) for biomedical applications or mitigating their cytotoxic effects require microscopic understanding of their interactions with cell membranes. Such insight is best obtained by studying model biomembranes...

scholarly journals Synovial Fluid Profile Dictates Nanopracticle Uptake into Cartilage- Implications of the Protein Corona for Novel Arthitis Treatments

2021 ◽  
Author(s):  
Ula von Mentzer ◽  
Tilia Selldén ◽  
LOISE Råberg ◽  
Gizem Erensoy ◽  
Anna-Karin Hultgård-Ekwall ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Drug Delivery ◽  
Synovial Fluid ◽  
Electrostatic Interactions ◽  
Fetal Calf Serum ◽  
Biological Fluids ◽  
Calf Serum ◽  
Protein Corona ◽  
Drug Delivery Vehicles ◽  
Drug Concentrations

<div>Intra-articular drug delivery strategies aiming to deliver drugs in diseases affected by cartilage-related issues are using electrostatic interactions to penetrate the dense cartilage matrix. This enables delivery of sufficient drug concentrations to the chondrocytes to mediate the desired therapeutic effect. As it is well known that size and charge of nanoparticles affects its interactions with the surrounding biological fluids, where proteins adsorb to the NP surface, resulting in a protein corona. There are, however, no studies investigating how the formed protein coronas affect cartilage uptake and subsequent cellular uptake, nor how they affect other cells present in the synovium of such diseases. Here, we explore the differences between the protein coronas that form when NP are incubated in synovial fluid from osteoarthritic and rheumatoid arthritis patients and compare this to results obtained using fetal calf serum (FCS), as guide for researchers working on joint drug delivery. We demonstrate that the protein corona indeed affects the uptake into cartilage, where there are major differences between the model proteins in fetal calf serum, as compared to synovial fluid from rheumatoid arthritis patients as well as osteoarthritis patients. The data suggests that when developing drug delivery vehicles for joint diseases that leverages electrostatic interactions and size, the interactions with proteins in the biological milieu is highly relevant to consider.</div>

scholarly journals Conformational-transited protein corona regulated cell-membrane penetration and induced cytotoxicity of ultrasmall Au nanoparticles

RSC Advances ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 4435-4444 ◽  
Author(s):  
Huayan Yang ◽  
Meng Wang ◽  
Yanmin Zhang ◽  
Feng Li ◽  
Shaoning Yu ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Cell Membranes ◽  
Au Nanoparticles ◽  
Protein Corona ◽  
Membrane Disruption ◽  
Membrane Penetration

This study demonstrate that the AuNP–HSA corona could penetrate cell membranes and companied by substantial membrane disruption. However, the ultrasmall AuNPs can be internalized by cells without the destruction of cell membranes.

scholarly journals Protein Corona Composition of Silica Nanoparticles in Complex Media: Nanoparticle Size does not Matter

Nanomaterials ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 240 ◽  
Author(s):  
Laurent Marichal ◽  
Géraldine Klein ◽  
Jean Armengaud ◽  
Yves Boulard ◽  
Stéphane Chédin ◽  
...  
Keyword(s):  
Silica Nanoparticles ◽  
Electrostatic Interactions ◽  
Large Scale ◽  
Driving Forces ◽  
Protein Corona ◽  
Sequence Length ◽  
Label Free ◽  
Curvature Effects ◽  
Proteomic Approach ◽  
Adsorbed Proteins

Biomolecules, and particularly proteins, bind on nanoparticle (NP) surfaces to form the so-called protein corona. It is accepted that the corona drives the biological distribution and toxicity of NPs. Here, the corona composition and structure were studied using silica nanoparticles (SiNPs) of different sizes interacting with soluble yeast protein extracts. Adsorption isotherms showed that the amount of adsorbed proteins varied greatly upon NP size with large NPs having more adsorbed proteins per surface unit. The protein corona composition was studied using a large-scale label-free proteomic approach, combined with statistical and regression analyses. Most of the proteins adsorbed on the NPs were the same, regardless of the size of the NPs. To go beyond, the protein physicochemical parameters relevant for the adsorption were studied: electrostatic interactions and disordered regions are the main driving forces for the adsorption on SiNPs but polypeptide sequence length seems to be an important factor as well. This article demonstrates that curvature effects exhibited using model proteins are not determining factors for the corona composition on SiNPs, when dealing with complex biological media.

Effect of protein corona on nanoparticle–plasma membrane and nanoparticle–biomimetic membrane interactions

2020 ◽  
Vol 7 (3) ◽  
pp. 963-974
Author(s):  
Lu Wang ◽  
Nicolas Hartel ◽  
Kaixuan Ren ◽  
Nicholas Alexander Graham ◽  
Noah Malmstadt
Keyword(s):  
Plasma Membrane ◽  
Systematic Study ◽  
Electrostatic Interactions ◽  
Plasma Membranes ◽  
Membrane Integrity ◽  
Protein Corona ◽  
Membrane Interactions ◽  
Biomimetic Membranes ◽  
Biomimetic Membrane

A systematic study of the protein corona's effect on nanoparticle–biomembrane electrostatic interactions. Nanoparticle adhesion and membrane integrity upon interaction were compared between plasma membranes and biomimetic membranes.

scholarly journals Differential Interactions of Chiral Nanocapsules with DNA

2021 ◽  
Vol 22 (2) ◽  
pp. 584
Author(s):  
Amani Zoabi ◽  
Katherine Margulis
Keyword(s):  
Electrostatic Interactions ◽  
Volatile Oil ◽  
Supramolecular Interactions ◽  
The Novel ◽  
Fourfold Increase ◽  
Dna Oligonucleotides ◽  
Oil In Water ◽  
Preferential Binding ◽  
Capsule Size ◽  
Modification Of The Surface

(1) Background: Chiral nanoparticular systems have recently emerged as a compelling platform for investigating stereospecific behavior at the nanoscopic level. We describe chiroselective supramolecular interactions that occur between DNA oligonucleotides and chiral polyurea nanocapsules. (2) Methods: We employ interfacial polyaddition reactions between toluene 2,4-diisocyanate and lysine enantiomers that occur in volatile oil-in-water nanoemulsions to synthesize hollow, solvent-free capsules with average sizes of approximately 300 nm and neutral surface potential. (3) Results: The resultant nanocapsules exhibit chiroptical activity and interact differentially with single stranded DNA oligonucleotides despite the lack of surface charge and, thus, the absence of significant electrostatic interactions. Preferential binding of DNA on d-polyurea nanocapsules compared to their l-counterparts is demonstrated by a fourfold increase in capsule size, a 50% higher rise in the absolute value of negative zeta potential (ζ-potential), and a three times lower free DNA concentration after equilibration with the excess of DNA. (4) Conclusions: We infer that the chirality of the novel polymeric nanocapsules affects their supramolecular interactions with DNA, possibly through modification of the surface morphology. These interactions can be exploited when developing carriers for gene therapy and theranostics. The resultant constructs are expected to be highly biocompatible due to their neutral potential and biodegradability of polyurea shells.

scholarly journals Synovial Fluid Profile Dictates Nanopracticle Uptake into Cartilage- Implications of the Protein Corona for Novel Arthitis Treatments

2021 ◽  
Author(s):  
Ula von Mentzer ◽  
Tilia Selldén ◽  
LOISE Råberg ◽  
Gizem Erensoy ◽  
Anna-Karin Hultgård-Ekwall ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Drug Delivery ◽  
Synovial Fluid ◽  
Electrostatic Interactions ◽  
Fetal Calf Serum ◽  
Biological Fluids ◽  
Calf Serum ◽  
Protein Corona ◽  
Drug Delivery Vehicles ◽  
Drug Concentrations

<div>Intra-articular drug delivery strategies aiming to deliver drugs in diseases affected by cartilage-related issues are using electrostatic interactions to penetrate the dense cartilage matrix. This enables delivery of sufficient drug concentrations to the chondrocytes to mediate the desired therapeutic effect. As it is well known that size and charge of nanoparticles affects its interactions with the surrounding biological fluids, where proteins adsorb to the NP surface, resulting in a protein corona. There are, however, no studies investigating how the formed protein coronas affect cartilage uptake and subsequent cellular uptake, nor how they affect other cells present in the synovium of such diseases. Here, we explore the differences between the protein coronas that form when NP are incubated in synovial fluid from osteoarthritic and rheumatoid arthritis patients and compare this to results obtained using fetal calf serum (FCS), as guide for researchers working on joint drug delivery. We demonstrate that the protein corona indeed affects the uptake into cartilage, where there are major differences between the model proteins in fetal calf serum, as compared to synovial fluid from rheumatoid arthritis patients as well as osteoarthritis patients. The data suggests that when developing drug delivery vehicles for joint diseases that leverages electrostatic interactions and size, the interactions with proteins in the biological milieu is highly relevant to consider.</div>

scholarly journals Molecular Modeling of Protein Corona Formation and Its Interactions with Nanoparticles and Cell Membranes for Nanomedicine Applications

Pharmaceutics ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 637
Author(s):  
Hwankyu Lee
Keyword(s):  
Surface Properties ◽  
Plasma Proteins ◽  
Cell Membranes ◽  
Competitive Adsorption ◽  
Critical Role ◽  
Protein Corona ◽  
Md Simulations ◽  
Atomic Scale ◽  
Adsorption And Desorption ◽  
Corona Formation

The conformations and surface properties of nanoparticles have been modified to improve the efficiency of drug delivery. However, when nanoparticles flow through the bloodstream, they interact with various plasma proteins, leading to the formation of protein layers on the nanoparticle surface, called protein corona. Experiments have shown that protein corona modulates nanoparticle size, shape, and surface properties and, thus, influence the aggregation of nanoparticles and their interactions with cell membranes, which can increases or decreases the delivery efficiency. To complement these experimental findings and understand atomic-level phenomena that cannot be captured by experiments, molecular dynamics (MD) simulations have been performed for the past decade. Here, we aim to review the critical role of MD simulations to understand (1) the conformation, binding site, and strength of plasma proteins that are adsorbed onto nanoparticle surfaces, (2) the competitive adsorption and desorption of plasma proteins on nanoparticle surfaces, and (3) the interactions between protein-coated nanoparticles and cell membranes. MD simulations have successfully predicted the competitive binding and conformation of protein corona and its effect on the nanoparticle–nanoparticle and nanoparticle–membrane interactions. In particular, simulations have uncovered the mechanism regarding the competitive adsorption and desorption of plasma proteins, which helps to explain the Vroman effect. Overall, these findings indicate that simulations can now provide predications in excellent agreement with experimental observations as well as atomic-scale insights into protein corona formation and interactions.

scholarly journals Do Cyclotides bind Lipid Bilayers: Molecular Dynamics Simulations of Kalata B1 and POPC/POPE membranes

2014 ◽  
Author(s):  
Neville Y. Forlemu ◽  
Patrick Coppock
Keyword(s):  
Molecular Dynamics ◽  
Lipid Bilayers ◽  
Electrostatic Interactions ◽  
Close Contact ◽  
Amino Acid Residues ◽  
Kalata B1 ◽  
Preferential Binding ◽  
Mechanism Of Interaction ◽  
Dynamics Simulations ◽  
Lipid Layers

Cyclotides are cyclic antimicrobial peptides (APs) that offer promising features for the development of efficient pharmaceutical therapies. Their efficacy is still hampered by lack of molecular details of their mechanism/mode of action. We have used unconstrained an all-atom molecular dynamics (MD) simulation to investigate the interactions between a representative cyclotide (kalata B1) and bilayers comprising 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol (POPG), and 1-palmitoyl-2-oleoylphosphatidylcholine (POPC) lipids. Kalata B1 is attracted to the surface of both lipid layers through close contact interactions. There is preferential binding on POPE,POPG layer as opposed to POPC mainly due to stronger electrostatic interactions. Kalata B1 in the last 60 ns of the simulation remains in close contact with the lipid headgroups of POPE using 5 amino acid residues (VAL, ASN, THR, GLU, TRP). This initial data suggest that these surface interactions promote peptide distribution similar to the carpet model mechanism of interaction.

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