Effect of protein corona on nanoparticle–plasma membrane and nanoparticle–biomimetic membrane interactions

2020 ◽  
Vol 7 (3) ◽  
pp. 963-974
Author(s):  
Lu Wang ◽  
Nicolas Hartel ◽  
Kaixuan Ren ◽  
Nicholas Alexander Graham ◽  
Noah Malmstadt
Keyword(s):  
Plasma Membrane ◽  
Systematic Study ◽  
Electrostatic Interactions ◽  
Plasma Membranes ◽  
Membrane Integrity ◽  
Protein Corona ◽  
Membrane Interactions ◽  
Biomimetic Membranes ◽  
Biomimetic Membrane

A systematic study of the protein corona's effect on nanoparticle–biomembrane electrostatic interactions. Nanoparticle adhesion and membrane integrity upon interaction were compared between plasma membranes and biomimetic membranes.

scholarly journals Species-specific differences in the susceptibility of fungi towards the antifungal protein AFP depend on C3 saturation of glycosylceramides

2019 ◽  
Author(s):  
Norman Paege ◽  
Dirk Warnecke ◽  
Simone Zäuner ◽  
Silke Hagen ◽  
Ana Rodrigues ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Fatty Acid ◽  
Mammalian Cells ◽  
Plasma Membranes ◽  
Membrane Integrity ◽  
Structural Difference ◽  
Inhibitory Effects ◽  
Chitin Synthesis ◽  
Fatty Acid Chain ◽  
Damage Response

AbstractAFP is an antimicrobial peptide (AMP) produced by the filamentous fungus Aspergillus giganteus and a very potent inhibitor of fungal growth without affecting the viability of bacteria, plant or mammalian cells. It targets chitin synthesis and causes plasma membrane permeabilization in many human and plant pathogenic fungi, but its exact mode of action is not known. We have recently proposed adoption of the “damage-response framework of microbial pathogenesis” put forward by Pirofksi and Casadevall in 1999 regarding the analysis of interactions between AMPs and microorganisms, thus, predicting that the cytotoxic capacity of a given AMP is relative and depends not only on the presence/absence of its target(s) in the host and the AMP concentration applied but also on other variables, such as microbial survival strategies. We show here using the examples of three filamentous fungi (Aspergillus niger, Aspergillus fumigatus, Fusarium graminearum) and two yeasts (Saccharomyces cerevisiae, Pichia pastoris) that the important parameters defining the AFP susceptibilities of these fungi are (i) the presence/absence of glycosylceramides, (ii) the presence/absence of Δ3(E)-desaturation of the fatty acid chain therein, and (iii) the (dis)ability of these fungi to respond to AFP inhibitory effects with the fortification of their cell walls via increased chitin and β-(1,3)-glucan synthesis. These observations support the adoption of the damage-response framework to holistically understand the outcome of AFP inhibitory effects.ImportanceOur data suggest a fundamental role of glycosylceramides in the susceptibility of fungi towards AFP. We discovered that only a minor structural difference in these molecules – the saturation level of their fatty acid chain, controlled by a 2-hydroxy fatty N-acyl-Δ3(E)-desaturase – is a key to understanding the inhibitory activity of AFP. As glycosylceramides are important components of fungal plasma membranes, we propose a model which links AFP-mediated inhibition of chitin synthesis in fungi with its potential to disturb plasma membrane integrity.

scholarly journals Chondroitin sulfate at the plasma membranes of cultured fibroblasts.

1983 ◽  
Vol 97 (4) ◽  
pp. 1288-1293 ◽  
Author(s):  
K Hedman ◽  
J Christner ◽  
I Julkunen ◽  
A Vaheri
Keyword(s):  
Plasma Membrane ◽  
Cell Surface ◽  
Chondroitin Sulfate ◽  
Electrostatic Interactions ◽  
Plasma Membranes ◽  
Chondroitin Sulfate Proteoglycan ◽  
Pericellular Matrix ◽  
Sulfate Proteoglycan ◽  
Cultured Fibroblasts ◽  
Outer Aspect

We have previously shown that in confluent human fibroblast cultures chondroitin sulfate proteoglycan is a component of the fibronectin-containing pericellular matrix fibers. In the present work the distribution of chondroitin sulfate was studied in subconfluent cell cultures using antibodies that bind to a chemically defined carbohydrate fragment of chondroitinase ABC-modified chondroitin sulfate proteoglycan. Using immunofluorescence microscopy, we observed, in addition to the fibrillar matrix staining, chondroitin sulfate diffusely distributed at the cell surface. In indirect immunoferritin electron microscopy this staining corresponded to patchy binding of ferritin close (24 nm) to the outer aspect of the plasma membrane. The patchy organization appeared uniform in all cell surfaces. The cell surface chondroitin sulfate could not be removed from the plasma membrane by agents that dissociate electrostatic interactions. These data show that in fibroblasts chondroitin sulfate is a component of the outer aspect of the plasma membrane, and raise the possibility of an integral plasma membrane chondroitin sulfate proteoglycan.

scholarly journals Species-Specific Differences in the Susceptibility of Fungi to the Antifungal Protein AFP Depend on C-3 Saturation of Glycosylceramides

mSphere ◽  
2019 ◽  
Vol 4 (6) ◽  
Author(s):  
Norman Paege ◽  
Dirk Warnecke ◽  
Simone Zäuner ◽  
Silke Hagen ◽  
Ana Rodrigues ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Fatty Acid ◽  
Mammalian Cells ◽  
Plasma Membranes ◽  
Membrane Integrity ◽  
Inhibitory Effects ◽  
Chitin Synthesis ◽  
Content Type ◽  
Fatty Acid Chain ◽  
Damage Response

ABSTRACT AFP is an antimicrobial peptide (AMP) produced by the filamentous fungus Aspergillus giganteus and is a very potent inhibitor of fungal growth that does not affect the viability of bacteria, plant, or mammalian cells. It targets chitin synthesis and causes plasma membrane permeabilization in many human- and plant-pathogenic fungi, but its exact mode of action is not known. After adoption of the “damage-response framework of microbial pathogenesis” regarding the analysis of interactions between AMPs and microorganisms, we have recently proposed that the cytotoxic capacity of a given AMP depends not only on the presence/absence of its target(s) in the host and the AMP concentration applied but also on other variables, such as microbial survival strategies. We show here using the examples of three filamentous fungi (Aspergillus niger, Aspergillus fumigatus, and Fusarium graminearum) and two yeasts (Saccharomyces cerevisiae and Pichia pastoris) that the important parameters defining the AFP susceptibilities of these fungi are (i) the presence/absence of glycosylceramides, (ii) the presence/absence of Δ3(E) desaturation of the fatty acid chain therein, and (iii) the (dis)ability of these fungi to respond to AFP inhibitory effects with the fortification of their cell walls via increased chitin and β-(1,3)-glucan synthesis. These observations support the idea of the adoption of the damage-response framework to holistically understand the outcome of AFP inhibitory effects. IMPORTANCE Our data suggest a fundamental role of glycosylceramides in the susceptibility of fungi to AFP. We discovered that only a minor structural difference in these molecules—namely, the saturation level of their fatty acid chain, controlled by a 2-hydroxy fatty N-acyl-Δ3(E)-desaturase—represents a key to understanding the inhibitory activity of AFP. As glycosylceramides are important components of fungal plasma membranes, we propose a model which links AFP-mediated inhibition of chitin synthesis in fungi with its potential to disturb plasma membrane integrity.

Membrane microdomains: lessons from microscopy

1995 ◽  
Vol 53 ◽  
pp. 776-777
Author(s):  
J.M. Robinson ◽  
J.M Oliver
Keyword(s):  
Spatial Distribution ◽  
Plasma Membrane ◽  
Epithelial Cells ◽  
Plasma Membranes ◽  
Cell Types ◽  
Imaging Modalities ◽  
Membrane Microdomains ◽  
Membrane Domains ◽  
Lateral Heterogeneity ◽  
Functional Importance

Specialized regions of plasma membranes displaying lateral heterogeneity are the focus of this Symposium. Specialized membrane domains are known for certain cell types such as differentiated epithelial cells where lateral heterogeneity in lipids and proteins exists between the apical and basolateral portions of the plasma membrane. Lateral heterogeneity and the presence of microdomains in membranes that are uniform in appearance have been more difficult to establish. Nonetheless a number of studies have provided evidence for membrane microdomains and indicated a functional importance for these structures.This symposium will focus on the use of various imaging modalities and related approaches to define membrane microdomains in a number of cell types. The importance of existing as well as emerging imaging technologies for use in the elucidation of membrane microdomains will be highlighted. The organization of membrane microdomains in terms of dimensions and spatial distribution is of considerable interest and will be addressed in this Symposium.

Comparision of DNA fragmentantion and plasma membrane integrity between chilled and frozen semen of bulls

2014 ◽  
Author(s):  
Mello Papa Patricia de ◽  
Carlos Ramires Neto ◽  
Priscilla Nascimento Guasti ◽  
Rosiara Rosaria Dias Maziero ◽  
Yame F R Sancler-Silva ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Membrane Integrity ◽  
Plasma Membrane Integrity ◽  
Frozen Semen

Pore-Spanning Plasma Membranes Derived from Giant Plasma Membrane Vesicles

2021 ◽  
Author(s):  
Nikolas K. Teiwes ◽  
Ingo Mey ◽  
Phila C. Baumann ◽  
Lena Strieker ◽  
Ulla Unkelbach ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Plasma Membranes ◽  
Membrane Vesicles ◽  
Plasma Membrane Vesicles

scholarly journals Plasma membrane integrity in health and disease: significance and therapeutic potential

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Catarina Dias ◽  
Jesper Nylandsted
Keyword(s):  
Plasma Membrane ◽  
Therapeutic Potential ◽  
Calcium Influx ◽  
Membrane Integrity ◽  
Cell Types ◽  
Physical Parameters ◽  
Membrane Repair ◽  
Plasma Membrane Integrity ◽  
Repair Mechanisms ◽  
And Function

AbstractMaintenance of plasma membrane integrity is essential for normal cell viability and function. Thus, robust membrane repair mechanisms have evolved to counteract the eminent threat of a torn plasma membrane. Different repair mechanisms and the bio-physical parameters required for efficient repair are now emerging from different research groups. However, less is known about when these mechanisms come into play. This review focuses on the existence of membrane disruptions and repair mechanisms in both physiological and pathological conditions, and across multiple cell types, albeit to different degrees. Fundamentally, irrespective of the source of membrane disruption, aberrant calcium influx is the common stimulus that activates the membrane repair response. Inadequate repair responses can tip the balance between physiology and pathology, highlighting the significance of plasma membrane integrity. For example, an over-activated repair response can promote cancer invasion, while the inability to efficiently repair membrane can drive neurodegeneration and muscular dystrophies. The interdisciplinary view explored here emphasises the widespread potential of targeting plasma membrane repair mechanisms for therapeutic purposes.

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