A fluorometric assay for lysosomal phospholipase A2 activity using fluorescence-labeled truncated oxidized phospholipid

2018 ◽  
Vol 549 ◽  
pp. 164-170 ◽  
Author(s):  
Akira Abe ◽  
Miki Hiraoka ◽  
James A. Shayman ◽  
Hiroshi Ohguro
Keyword(s):  
Phospholipase A2 ◽  
Fluorometric Assay ◽  
Phospholipase A2 Activity ◽  
Oxidized Phospholipid

A continuous fluorometric assay for phospholipase A2 activity in brain cytosol

2000 ◽  
Vol 100 (1-2) ◽  
pp. 127-133 ◽  
Author(s):  
Danny E. Yarger ◽  
Casey B. Patrick ◽  
Stanley I. Rapoport ◽  
Eric J. Murphy
Keyword(s):  
Phospholipase A2 ◽  
Fluorometric Assay ◽  
Phospholipase A2 Activity ◽  
Brain Cytosol

A rapid fluorometric assay for the determination of serum phospholipase A2 activity

2009 ◽  
Vol 91 (1) ◽  
pp. 82-84 ◽  
Author(s):  
M. Merchant ◽  
J. Small ◽  
R. Falconi ◽  
B.J. Crookshank
Keyword(s):  
Phospholipase A2 ◽  
Fluorometric Assay ◽  
Phospholipase A2 Activity

scholarly journals Oxidation of Peroxiredoxin 6 in the Presence of GSH Increases its Phospholipase A2 Activity at Cytoplasmic pH

Antioxidants ◽  
2018 ◽  
Vol 8 (1) ◽  
pp. 4 ◽  
Author(s):  
Suiping Zhou ◽  
Chandra Dodia ◽  
Sheldon Feinstein ◽  
Sandra Harper ◽  
Henry Forman ◽  
...  
Keyword(s):  
Phospholipase A2 ◽  
Tryptophan Fluorescence ◽  
Anaerobic Conditions ◽  
Cell Cytoplasm ◽  
Cytosolic Ph ◽  
Effect Analysis ◽  
Peroxiredoxin 6 ◽  
Phospholipase A2 Activity ◽  
Oxidized Phospholipid

The expression of the phospholipase A2 activity (aiPLA2) of peroxiredoxin 6 (Prdx6) in the cell cytoplasm is physiologically relevant for the repair of peroxidized cell membranes, but aiPLA2 assay in vitro indicates that, unlike assay at pH 4, activity at cytosolic pH is essentially absent with non-oxidized substrate. However, the addition of glutathione (GSH) to the assay medium significantly increased aiPLA2 activity at cytosolic pH, while oxidized GSH (GSSG) and several other thiols had no effect. By mass spectroscopy (ESI MS), the addition of GSH to Prdx6 paradoxically led to oxidation of its conserved Cys47 residue to a sulfinic acid. The effect of GSH on PLA2 activity was abolished by incubation under anaerobic conditions, confirming that auto-oxidation of the protein was the mechanism for the GSH effect. Analysis by circular dichroism (CD) and tryptophan fluorescence showed alterations of the protein structure in the presence of GSH. Independently of GSH, the oxidation of Prdx6 by exposure to H2O2 or the presence of oxidized phospholipid as substrate also significantly increased aiPLA2 activity at pH 7. We conclude that the oxidation of the peroxidatically active Cys47 of Prdx6 results in an increase of aiPLA2 activity at pH 7 without effect on the activity of the enzyme at pH 4.

Study of phospholipase A2 activity in umbilical endothelial cells in toxemia of pregnancy.

1987 ◽  
Vol 18 (6) ◽  
pp. 582-588
Author(s):  
Hiroyuki SEKI ◽  
Toshiharu JIMBO ◽  
Kazuo SATOH
Keyword(s):  
Endothelial Cells ◽  
Phospholipase A2 ◽  
Phospholipase A2 Activity
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