scholarly journals Development of a simple HPLC–UV method for the determination of the hepatitis C virus inhibitor simeprevir in human plasma

2016 ◽  
Vol 121 ◽  
pp. 197-203 ◽  
Author(s):  
Giulio Nannetti ◽  
Silvana Pagni ◽  
Saverio G. Parisi ◽  
Alfredo Alberti ◽  
Arianna Loregian ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Human Plasma ◽  
Virus Inhibitor

scholarly journals Stress stability study of simeprevir, a hepatitis C virus inhibitor, using feasible TLC-spectro-densitometry: application to pharmaceutical dosage form and human plasma

RSC Advances ◽  
2020 ◽  
Vol 10 (36) ◽  
pp. 21100-21107 ◽  
Author(s):  
Bassam Shaaban Mohammed ◽  
Amal E. Hamad ◽  
Sayed M. Derayea
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Human Plasma ◽  
Dosage Form ◽  
Direct Action ◽  
Stability Study ◽  
Pharmaceutical Dosage Form ◽  
Virus Inhibitor

Simeprevir is one of the newest direct action anti-hepatitis C drugs.

Development and validation of a highly sensitive second derivative synchronous fluorescence spectroscopic method for the simultaneous determination of elbasvir and grazoprevir in pharmaceutical preparation and human plasma

2020 ◽  
Vol 44 (43) ◽  
pp. 18679-18685
Author(s):  
Khalid A. M. Attia ◽  
Ahmed El-Olemy ◽  
Sherif Ramzy ◽  
Ahmed H. Abdelazim ◽  
Mohamed A. Hasan ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Simultaneous Determination ◽  
Human Plasma ◽  
Pharmaceutical Preparation ◽  
Spectroscopic Method ◽  
Second Derivative ◽  
Highly Sensitive ◽  
Development And Validation

Elbasvir and grazoprevir combination has been newly approved for the treatment of patients infected with hepatitis C virus.

Development and Validation of a New Method for the Determination of Anti-hepatitis C Agent Simeprevir in Human Plasma using HPLC with Fluorescence Detection

2020 ◽  
Vol 16 (4) ◽  
pp. 428-435
Author(s):  
Ahmed F.A. Youssef ◽  
Yousry M. Issa ◽  
Kareem M. Nabil
Keyword(s):  
Hepatitis C ◽  
Human Plasma ◽  
Fluorescence Detection ◽  
Internal Standard ◽  
Protein Precipitation ◽  
Assay Method ◽  
Fluorescence Detector ◽  
Limit Of Quantification ◽  
Relative Standard

Background: Simeprevir is one of the recently discovered drugs for treating hepatitis C which is one of the major diseases across the globe. Objective: The present study involves the development of a new and unique High-Performance Liquid Chromatography (HPLC) method using fluorescence detection for the determination of simeprevir (SIM) in human plasma. Methods: Two methods of extractions were tested, protein precipitation using acetonitrile and liquidliquid extraction. A 25 mM dipotassium hydrogen orthophosphate (pH 7.0)/ACN (50/50; v/v), was used as mobile phase and C18 reversed phase column as the stationary phase. The chromatographic conditions were optimized and the concentration of simeprevir was determined by using the fluorescence detector. Cyclobenzaprine was used as an internal standard. Results: Recovery of the assay method based on protein precipitation was up to 100%. Intra-day and inter-day accuracies range from 92.30 to 107.80%, with Relative Standard Deviation (RSD) range 1.65-8.02%. The present method was successfully applied to a pharmacokinetic study where SIM was administered as a single dose of 150 mg SIM/capsule (Olysio®) to healthy individuals. Conclusion: This method exhibits high sensitivity with a low limit of quantification 10 ng mL-1, good selectivity using fluorescence detection, wide linear application range 10-3000 ng mL-1, good recovery and highly precise and validation results. The developed method can be applied in routine analysis for real samples.

Binding Mode Determination of Benzimidazole Inhibitors of the Hepatitis C Virus RNA Polymerase by a Structure and Dynamics Strategy

2004 ◽  
Vol 116 (33) ◽  
pp. 4406-4411 ◽  
Author(s):  
Steven R. LaPlante ◽  
Araz Jakalian ◽  
Norman Aubry ◽  
Yves Bousquet ◽  
Jean-Marie Ferland ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Rna Polymerase ◽  
Binding Mode ◽  
Hepatitis C Virus Rna ◽  
Structure And Dynamics ◽  
Virus Rna
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