Microenvironment improvement protocol for the sensitive spectrofluorimetric determination of an hepatitis C virus antiviral (Simeprevir): application to human plasma

Luminescence ◽  
2020 ◽  
Vol 35 (3) ◽  
pp. 393-399
Author(s):  
Sayed M. Derayea ◽  
Mohamed A. El Hamd ◽  
Sayed Ali ◽  
Ebtihal Samir
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Human Plasma ◽  
Spectrofluorimetric Determination

Development and validation of a highly sensitive second derivative synchronous fluorescence spectroscopic method for the simultaneous determination of elbasvir and grazoprevir in pharmaceutical preparation and human plasma

2020 ◽  
Vol 44 (43) ◽  
pp. 18679-18685
Author(s):  
Khalid A. M. Attia ◽  
Ahmed El-Olemy ◽  
Sherif Ramzy ◽  
Ahmed H. Abdelazim ◽  
Mohamed A. Hasan ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Simultaneous Determination ◽  
Human Plasma ◽  
Pharmaceutical Preparation ◽  
Spectroscopic Method ◽  
Second Derivative ◽  
Highly Sensitive ◽  
Development And Validation

Elbasvir and grazoprevir combination has been newly approved for the treatment of patients infected with hepatitis C virus.

scholarly journals Development of a simple HPLC–UV method for the determination of the hepatitis C virus inhibitor simeprevir in human plasma

2016 ◽  
Vol 121 ◽  
pp. 197-203 ◽  
Author(s):  
Giulio Nannetti ◽  
Silvana Pagni ◽  
Saverio G. Parisi ◽  
Alfredo Alberti ◽  
Arianna Loregian ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Human Plasma ◽  
Virus Inhibitor

Development and Validation of a New Method for the Determination of Anti-hepatitis C Agent Simeprevir in Human Plasma using HPLC with Fluorescence Detection

2020 ◽  
Vol 16 (4) ◽  
pp. 428-435
Author(s):  
Ahmed F.A. Youssef ◽  
Yousry M. Issa ◽  
Kareem M. Nabil
Keyword(s):  
Hepatitis C ◽  
Human Plasma ◽  
Fluorescence Detection ◽  
Internal Standard ◽  
Protein Precipitation ◽  
Assay Method ◽  
Fluorescence Detector ◽  
Limit Of Quantification ◽  
Relative Standard

Background: Simeprevir is one of the recently discovered drugs for treating hepatitis C which is one of the major diseases across the globe. Objective: The present study involves the development of a new and unique High-Performance Liquid Chromatography (HPLC) method using fluorescence detection for the determination of simeprevir (SIM) in human plasma. Methods: Two methods of extractions were tested, protein precipitation using acetonitrile and liquidliquid extraction. A 25 mM dipotassium hydrogen orthophosphate (pH 7.0)/ACN (50/50; v/v), was used as mobile phase and C18 reversed phase column as the stationary phase. The chromatographic conditions were optimized and the concentration of simeprevir was determined by using the fluorescence detector. Cyclobenzaprine was used as an internal standard. Results: Recovery of the assay method based on protein precipitation was up to 100%. Intra-day and inter-day accuracies range from 92.30 to 107.80%, with Relative Standard Deviation (RSD) range 1.65-8.02%. The present method was successfully applied to a pharmacokinetic study where SIM was administered as a single dose of 150 mg SIM/capsule (Olysio®) to healthy individuals. Conclusion: This method exhibits high sensitivity with a low limit of quantification 10 ng mL-1, good selectivity using fluorescence detection, wide linear application range 10-3000 ng mL-1, good recovery and highly precise and validation results. The developed method can be applied in routine analysis for real samples.

Binding Mode Determination of Benzimidazole Inhibitors of the Hepatitis C Virus RNA Polymerase by a Structure and Dynamics Strategy

2004 ◽  
Vol 116 (33) ◽  
pp. 4406-4411 ◽  
Author(s):  
Steven R. LaPlante ◽  
Araz Jakalian ◽  
Norman Aubry ◽  
Yves Bousquet ◽  
Jean-Marie Ferland ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Rna Polymerase ◽  
Binding Mode ◽  
Hepatitis C Virus Rna ◽  
Structure And Dynamics ◽  
Virus Rna

On-chip purification and detection of hepatitis C virus RNA from human plasma

2016 ◽  
Vol 208 ◽  
pp. 54-61 ◽  
Author(s):  
V. Vaghi ◽  
C. Potrich ◽  
L. Pasquardini ◽  
L. Lunelli ◽  
L. Vanzetti ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Human Plasma ◽  
Hepatitis C Virus Rna ◽  
Virus Rna ◽  
On Chip
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