Histological evaluation of the healing process of autografted mandibular bone defects in rats under treatment with zoledronate

2019 ◽  
Vol 47 (11) ◽  
pp. 1779-1786
Author(s):  
Mohammad Zandi ◽  
Arash Dehghan ◽  
Ideh Talimkhani ◽  
Leila Rezaeian ◽  
Naser Mohammad Gholi Mezerji
Keyword(s):  
Healing Process ◽  
Bone Defects ◽  
Histological Evaluation ◽  
Mandibular Bone

The Routine and Specialised Staining for the Histologic Evaluation of Autogenous Mandibular Bone Grafts An experimental study

2018 ◽  
Vol 69 (5) ◽  
pp. 1106-1109 ◽  
Author(s):  
Victor Nimigean ◽  
Alexandru Poll ◽  
Vanda Roxana Nimigean ◽  
Simona Andreea Moraru ◽  
Daniela Gabriela Badita ◽  
...  
Keyword(s):  
Experimental Study ◽  
Bone Grafts ◽  
Bone Defects ◽  
Histological Evaluation ◽  
Oral Rehabilitation ◽  
Mandibular Bone ◽  
Maxillary Bone ◽  
Donor Region ◽  
Histologic Evaluation ◽  
Mandibular Body

Oral rehabilitation by dental implants is a routine treatment in the common dental practice, and volume reconstruction in cases of advanced alveolar ridge atrophy using bone autografts has become a frequently used therapeutic procedure. The study presents a histological evaluation of autogenous mandibular bone grafts integration in surgically created maxillary bone defects. Seven domestic adult dogs, Canis Familiaris were used in the study. Work methodology was established through maxillary and mandibular morphometry, the donor region being the posterior mandibular body, and the recipient region being the lateral body of the maxilla. In the experimental study, we simulated two bilateral maxillary bone defects, which were augmented with mandibular corticocancellous bone grafts. Biological samples containing the target areas were collected 90-100 days after grafting and the subsequent preparation method of the samples for histological analysis was the standard one.The histological results showed the successful integration and the beneficial effect of corticocancellous autogenous mandibular bone grafts applied in maxillary sites.

scholarly journals Octacalcium phosphate/gelatin composite facilitates bone regeneration of critical-sized mandibular defects in rats: A quantitative study

2019 ◽  
Vol 13 (4) ◽  
pp. 258-266
Author(s):  
Fereydoon Sargolzaei-Aval ◽  
Eshagh Ali Saberi ◽  
Mohammad Reza Arab ◽  
Narjes Sargolzaei ◽  
Tayebeh Sanchooli ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Volume Fraction ◽  
Healing Process ◽  
Octacalcium Phosphate ◽  
Bone Defects ◽  
Control Group ◽  
Sprague Dawley ◽  
Mandibular Bone ◽  
Significance Level ◽  
Significant Difference

Background. Regeneration of bone defects remains a challenge for maxillofacial surgeons. The present study aimed to compare the effects of octacalcium phosphate (OCP) and the combination of octacalcium phosphate/gelatin (OCP/Gel) on mandibular bone regeneration in rats Methods. In the present study, 36 male Sprague-Dawley rats were used. The animals were randomly assigned to the following experimental groups: OCP (n=12), OCP/Gel (n=12), and the control group (n=12). Defects were created in the rat mandibles and filled with 10 mg of OCP and OCP/Gel disks in the experimental groups. In the control group, however, no substance was administered. Samples were taken on days 7, 14, 21 and 56, respectively, after the implantation. Sections (5 µ) were prepared and stained by H&E. The sections were studied, and the volume fraction of newly formed bone was measured by Dunnett's T3 test based on the significance level (P=0.05). Results. In the experimental groups, the new bone formation began from the margin of defects 7‒14 days after the implantation. During the healing process, the newly formed bone healed a larger area of the defects and grew structurally. In the control group, the defects were primarily filled with dense connective tissue, and only a small amount of new bone was formed. The present study showed a statistically significant difference in the volume of newly formed bone between the experimental groups and the control group (P<0.001). Conclusion. OCP/Gel composite can be beneficial in the healing process of mandibular bone defects.

scholarly journals Effect of Systemic Zoledronic Acid Dosing Regimens on Bone Regeneration in Osteoporotic Rats

2021 ◽  
Vol 11 (4) ◽  
pp. 1906
Author(s):  
Marwa Y. Shaheen ◽  
Amani M. Basudan ◽  
Abdurahman A. Niazy ◽  
Jeroen J. J. P. van den Beucken ◽  
John A. Jansen ◽  
...  
Keyword(s):  
Bone Graft ◽  
Bone Grafting ◽  
Femoral Condyle ◽  
Bone Defects ◽  
Histological Evaluation ◽  
Favorable Effect ◽  
Osteoporotic Bone ◽  
Bovine Bone ◽  
Graft Placement ◽  
Regeneration Of Bone

The aim of this study was to evaluate the regeneration of bone defects created in the femoral condyle of osteoporotic rats, following intravenous (IV) zoledronate (ZA) treatment in three settings: pre-bone grafting (ZA-Pre), post-bone grafting (ZA-Post), and pre- plus post-bone grafting (ZA-Pre+Post). Twenty-four female Wistar rats were ovariectomized (OVX). After 12 weeks, bone defects were created in the left femoral condyle. All defects were grafted with a particulate inorganic cancellous bovine bone substitute. ZA (0.04 mg/kg, weekly) was administered to six rats 4 weeks pre-bone graft placement. To another six rats, ZA was given post-bone graft placement creation and continued for 6 weeks. Additional six rats received ZA treatment pre- and post-bone graft placement. Control animals received weekly saline intravenous injections. At 6 weeks post-bone graft placement, samples were retrieved for histological evaluation of the bone area percentage (BA%) and remaining bone graft percentage (RBG%). BA% for ZA-Pre (50.1 ± 3.5%) and ZA-Post (49.2 ± 8.2%) rats was significantly increased compared to that of the controls (35.4 ± 5.4%, p-value 0.031 and 0.043, respectively). In contrast, ZA-Pre+Post rats (40.7 ± 16.0%) showed similar BA% compared to saline controls (p = 0.663). For RBG%, all experimental groups showed similar results ranging from 36.3 to 47.1%. Our data indicate that pre- or post-surgical systemic IV administration of ZA improves the regeneration of bone defects grafted with inorganic cancellous bovine-bone particles in osteoporotic bone conditions. However, no favorable effect on bone repair was seen for continued pre- plus post-surgical ZA treatment.

scholarly journals Longitudinal in vivo evaluation of bone regeneration by combined measurement of multi-pinhole SPECT and micro-CT for tissue engineering

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Philipp S. Lienemann ◽  
Stéphanie Metzger ◽  
Anna-Sofia Kiveliö ◽  
Alain Blanc ◽  
Panagiota Papageorgiou ◽  
...  
Keyword(s):  
Computed Tomography ◽  
High Resolution ◽  
Bone Formation ◽  
Bone Regeneration ◽  
Bone Defects ◽  
Histological Evaluation ◽  
Biological Processes ◽  
Micro Ct ◽  
Pinhole Spect

Abstract Over the last decades, great strides were made in the development of novel implants for the treatment of bone defects. The increasing versatility and complexity of these implant designs request for concurrent advances in means to assess in vivo the course of induced bone formation in preclinical models. Since its discovery, micro-computed tomography (micro-CT) has excelled as powerful high-resolution technique for non-invasive assessment of newly formed bone tissue. However, micro-CT fails to provide spatiotemporal information on biological processes ongoing during bone regeneration. Conversely, due to the versatile applicability and cost-effectiveness, single photon emission computed tomography (SPECT) would be an ideal technique for assessing such biological processes with high sensitivity and for nuclear imaging comparably high resolution (<1 mm). Herein, we employ modular designed poly(ethylene glycol)-based hydrogels that release bone morphogenetic protein to guide the healing of critical sized calvarial bone defects. By combined in vivo longitudinal multi-pinhole SPECT and micro-CT evaluations we determine the spatiotemporal course of bone formation and remodeling within this synthetic hydrogel implant. End point evaluations by high resolution micro-CT and histological evaluation confirm the value of this approach to follow and optimize bone-inducing biomaterials.

scholarly journals Preliminary in Vivo Evaluation of Bone Healing in Critical Size Bone Defects Implanted with an Injectable Calcium Phosphate Bone Cement (Osteopaste)

2017 ◽  
Vol 16 (1) ◽  
Author(s):  
Che Nor Zarida Che Seman ◽  
Zamzuri Zakaria ◽  
Zunariah Buyong ◽  
Mohd Shukrimi Awang ◽  
Ahmad Razali Md Ralib @ Md Raghib
Keyword(s):  
Calcium Phosphate ◽  
Bone Cement ◽  
Bone Tissue ◽  
Bone Repair ◽  
Critical Size ◽  
Healing Process ◽  
Bone Defects ◽  
Calcium Phosphate Bone Cement ◽  
Rabbit Tibia

Introduction: A novel injectable calcium phosphate bone cement (osteopaste) has been developed. Its potential application in orthopaedics as a filler of bone defects has been studied. The biomaterial was composed of tetra-calcium phosphate (TTCP) and tricalcium phosphate (TCP) powder. The aim of the present study was to evaluate the healing process of osteopaste in rabbit tibia. Materials and method: The implantation procedure was carried out on thirty-nine of New Zealand white rabbits. The in vivo bone formation was investigated by either implanting the Osteopaste, Jectos or MIIG – X3 into a critical size defect (CSD) model in the proximal tibial metaphysis. CSD without treatment served as negative control. After 1 day, 6 and 12 weeks, the rabbits were euthanized, the bone were harvested and subjected for analysis. Results: Radiological images and histological sections revealed integration of implants with bone tissue with no signs of graft rejection. There was direct contact between osteopaste material and host bone. The new bone was seen bridging the defect. Conclusion: The result showed that Osteopaste could be a new promising biomaterial for bone repair and has a potential in bone tissue engineering.

scholarly journals Current approach to bone augmentation with allogeneic cortical graft: A case report

2021 ◽  
Vol 11 (Suppl. 1) ◽  
pp. 299-302
Author(s):  
Utku Nezih Yılmaz ◽  
Fatma Eriş Derkuş
Keyword(s):  
Case Report ◽  
Dental Implant ◽  
Soft Tissues ◽  
Alveolar Bone ◽  
Healing Process ◽  
Bone Defects ◽  
Current Approach ◽  
Autogenous Bone ◽  
Bone Augmentation ◽  
Alveolar Crest

Aim: Today, dental implant applications have become the most preferred option in the treatment of tooth deficiencies. Long-term successful results in dental implant applications depend largely on the volume and quality of the hard and soft tissues in the relevant region. Insufficient soft tissues and alveolar crest resorption complicate implant applications. Grafts and additional surgical procedures are required to compensate for resorption and to provide bone augmentation. Shell technique, one of the augmentation methods used in the treatment of alveolar bone defects, is an important procedure for guided bone regeneration. The purpose of this case report is to describe the treatment of vertical and horizontal bone loss with the Shell technique using allogeneic cortical grafts. Methodology: A 58-year-old female patient without any systemic disease was admitted to our clinic with the complaint of tooth loss in the right posterior mandibular region. In the intraoral and radiological examinations, it was determined that the bone volume in the relevant region was not sufficient for dental implant. Two-stage surgical treatment was planned for the patient. First, vertical and horizontal bone defects were augmented with allogeneic cortical graft application under local anesthesia. After the healing process, dental implants were placed in the sufficient volume of the alveolar bone and the patient's treatment was completed. Conclusion: Allogeneic grafts in the treatment of alveolar crest defects; it is a good alternative to autogenous bone grafts,there is no need for a second surgical field and the resulting reduction in morbidity.   How to cite this article: Eriş Derkuş F, Yılmaz UN. Current approach to bone augmentation with allogeneic cortical graft: A case report. Int Dent Res 2021;11(Suppl.1):299-302. https://doi.org/10.5577/intdentres.2021.vol11.suppl1.44     Linguistic Revision: The English in this manuscript has been checked by at least two professional editors, both native speakers of English.

A Biomechanical Comparison of Three Miniature Locking Plate Systems in a Rabbit Radial and Ulnar Fracture Model

2019 ◽  
Vol 32 (04) ◽  
pp. 297-304 ◽  
Author(s):  
Harue Takizawa ◽  
Muneki Honnami ◽  
Takamasa Sakai ◽  
Akari Sasaki ◽  
Ayumi Sakamoto ◽  
...  
Keyword(s):  
Fracture Healing ◽  
Locking Plate ◽  
Healing Process ◽  
Biomechanical Testing ◽  
Histological Evaluation ◽  
Fracture Stabilization ◽  
Significant Difference ◽  
Ulnar Fracture ◽  
Fracture Models

Objective The aim of this study was to evaluate the biomechanical properties of three different miniature locking plate systems used to fixate radial and ulnar fractures in toy breed dogs. Implant size, shape, material and locking systems differ, and their influence on the fracture healing process is unknown. In the present study, we aimed to investigate this matter in vivo using rabbit radial and ulnar fracture models. Study Design Eighteen rabbits were randomly divided into three groups, and the left radius and ulna were osteotomized to create fracture models. The osteotomies were then fixated using either the TITAN LOCK 1.5, Fixin micro or LCP 1.5 system. Radiographs were obtained 2, 3 and 4 weeks after surgery. Four weeks after surgery, the radiuses were collected and used for biomechanical testing or histological examinations. Results During the 4 weeks of observation, no adverse effects due to the implants occurred. The radiographic scores in each group did not differ significantly at any time point. The maximum load in the LCP group was significantly higher than that in the TITAN and Fixin groups. There was no significant difference in bending stiffness or work to failure among the groups. Initial fracture healing via woven bone was evident at histological evaluation. Conclusions All three miniature locking plate systems provided adequate fracture stabilization 4 weeks after surgery, despite their differences, in rabbit models.

scholarly journals Effect of platelet-rich plasma on implant bone defects in rabbits through the FAK/PI3K/AKT signaling pathway

2019 ◽  
Vol 14 (1) ◽  
pp. 311-317
Author(s):  
Wei Liu ◽  
Ben Chen ◽  
Youyang Zheng ◽  
Yuehua Shi ◽  
Zhuojin Shi
Keyword(s):  
Growth Factor ◽  
Bone Healing ◽  
Alveolar Bone ◽  
Platelet Rich Plasma ◽  
Healing Process ◽  
Bone Defects ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Tissue Healing ◽  
Bone Healing Process

AbstractPlatelet-rich plasma (PRP) has been shown to be a beneficial growth factor for bone tissue healing and is used in implantology. The aim of this study was to investigate the effects of PRP on bone defects in rabbits. Twenty rabbits were used to establish the implant bone defect model in this study. An intrabony defect (5mm × 5mm × 3mm) was created in alveolar bone in the lower jar of each rabbit. The wound was treated with PRP. The expression of platelet-derived growth factor BB (PDGFBB) was assessed by enzyme-linked immunosorbent assay (ELISA). Focal adhesion kinase (FAK) and related phosphatidylinositol 3-kinase (PI3K)/AKT (protein kinase B) levels were measured by Western blot. The results show that PRP could significantly improve the bone healing process when compared with control, and 10% PRP could markedly increase fibroblast proliferation 48-h post treatment. PDGFBB was higher in the PRP group than that in the control group. PRP treatment also could elevate the phosphorylation of FAK and PI3K/AKT, although the inhibitor of PDGFR could reverse this trend. These results suggest that PRP treatment improves the bone healing process through the FAK/PI3K/AKT pathway.

scholarly journals Healing Process Following Application of Set or Fresh Mineral Trioxide Aggregate as a Root-End Filling Material

2011 ◽  
Vol 05 (01) ◽  
pp. 019-023 ◽  
Author(s):  
Mehdi Habibi ◽  
Jamileh Ghoddusi ◽  
Ataollah Habibi ◽  
Nooshin Mohtasham
Keyword(s):  
Healing Process ◽  
Mineral Trioxide Aggregate ◽  
Histological Evaluation ◽  
Filling Material ◽  
Chi Square ◽  
Unsuccessful Attempt ◽  
Gutta Percha ◽  
Significant Difference ◽  
Chronic Abscess ◽  
The Difference

ABSTRACTObjectives: An unsuccessful attempt to reach the apical area or to place the retrograde material is a major difficulty in periradicular surgery. The aim of this study was to compare the histological evaluation of the healing process following an orthograde versus a retrograde application of mineral trioxide aggregate (MTA) as a root-end filling material during apical surgery on cats’ teeth in order to find out whether orthograde placement of MTA before surgery can be used instead of retrograde placement during surgery. Methods: In this experimental study, 24 canine teeth in 12 mature and healthy cats were filled with either MTA or gutta-percha in an orthograde manner. Two weeks later, the teeth with MTA were surgically exposed and resected to the set-MTA within the canals. The teeth previously filled by gutta-percha were also surgically exposed, and retrograde cavities were prepared at the root ends and filled with fresh-MTA. After 8 weeks, the animals were euthanized by vital perfusion. Six-micron histological slices were prepared from samples, stained by Hematoxylin & Eosin, and histologically studied by means of a light microscope. The collected data was analyzed by the Chi-square and the T-test. Results: One of the samples in the fresh-MTA group was omitted during processing because of inappropriate sectioning. In the set-MTA group, 5 out of 12 showed chronic abscess, while in the fresh-MTA group, 2 out of 11 were discovered to have chronic abscess; however, no significant difference was observed (P>.05). Hard tissue healing (cementum, bone, cementum + bone formation) in the set-MTA and fresh-MTA groups were 7 out of 12 and 9 out of 11, respectively. While healing seemed more likely to occur in the fresh-MTA group, the difference was statistically insignificant (P>.05). The magnitude of bone, cementum, or bone and cementum formation showed slight differences between the two groups; however, the figures failed to show any marked differences (P>.05). Conclusions: Orthograde placement of MTA could be used as an obturation material before surgery. In this way, after root-end resection, there would be no need for root-end preparation and filling procedures. (Eur J Dent 2011;5:19-23)

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