scholarly journals Octacalcium phosphate/gelatin composite facilitates bone regeneration of critical-sized mandibular defects in rats: A quantitative study

2019 ◽  
Vol 13 (4) ◽  
pp. 258-266
Author(s):  
Fereydoon Sargolzaei-Aval ◽  
Eshagh Ali Saberi ◽  
Mohammad Reza Arab ◽  
Narjes Sargolzaei ◽  
Tayebeh Sanchooli ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Volume Fraction ◽  
Healing Process ◽  
Octacalcium Phosphate ◽  
Bone Defects ◽  
Control Group ◽  
Sprague Dawley ◽  
Mandibular Bone ◽  
Significance Level ◽  
Significant Difference

Background. Regeneration of bone defects remains a challenge for maxillofacial surgeons. The present study aimed to compare the effects of octacalcium phosphate (OCP) and the combination of octacalcium phosphate/gelatin (OCP/Gel) on mandibular bone regeneration in rats Methods. In the present study, 36 male Sprague-Dawley rats were used. The animals were randomly assigned to the following experimental groups: OCP (n=12), OCP/Gel (n=12), and the control group (n=12). Defects were created in the rat mandibles and filled with 10 mg of OCP and OCP/Gel disks in the experimental groups. In the control group, however, no substance was administered. Samples were taken on days 7, 14, 21 and 56, respectively, after the implantation. Sections (5 µ) were prepared and stained by H&E. The sections were studied, and the volume fraction of newly formed bone was measured by Dunnett's T3 test based on the significance level (P=0.05). Results. In the experimental groups, the new bone formation began from the margin of defects 7‒14 days after the implantation. During the healing process, the newly formed bone healed a larger area of the defects and grew structurally. In the control group, the defects were primarily filled with dense connective tissue, and only a small amount of new bone was formed. The present study showed a statistically significant difference in the volume of newly formed bone between the experimental groups and the control group (P<0.001). Conclusion. OCP/Gel composite can be beneficial in the healing process of mandibular bone defects.

scholarly journals Histological Assessment of Bone Regeneration by Octacalcium Phosphate and Bone Matrix Gelatin Composites in a Rat Mandibular Defect Model

2018 ◽  
Author(s):  
Fereydoon Sargolzaei Aval ◽  
Eshaghali Saberi ◽  
Mohammad Reza Arab ◽  
Narjes Sargolzaei ◽  
Esmaeel Zare ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Regeneration ◽  
Bone Matrix ◽  
Octacalcium Phosphate ◽  
Bone Defects ◽  
Osteogenic Potential ◽  
Control Group ◽  
New Bone Formation ◽  
Mandibular Bone

Objective: Regeneration of bone defects remains a challenge for maxillofacial and reparative surgeons. The purpose of this histological study was to assess the osteogenic potential of octacalcium phosphate (OCP) and bone matrix gelatin (BMG) alone and in combination in artificially created mandibular bone defects in rats. The quality of the newly formed bone was also evaluated. Methods: Thirty-six male Sprague Dawley rats (6-8 weeks old with 120-150 g weight) were randomly divided into four groups. Defects (3 mm in diameter and 2 mm in depth) were created in the mandible of rats and filled with 6 mg of OCP, BMG or a combination of both (1/4 ratio), respectively. Defects were left unfilled in the control group. To assess osteoinduction and bone regeneration and determine the quality of the newly formed bone, tissue specimens were harvested at seven, 14, and 21 days post-implantation. The specimens were processed, stained with hematoxylin and eosin (H&amp;E) and histologically analyzed under light microscopy. Results: In the experimental groups, new bone formation was initiated at the margins of defects from seventh day after implantation. At the end of the study period, the amount of the newly formed bone increased and the bone was relatively mature. Osteoinduction and new bone formation were greater in OCP/BMG group. In the control group, slight amount of new bone had been formed at the defect margins (next to host bone) on day 21. Conclusion: Combination of OCP/BMG may serve as an optimal biomaterial for treatment of mandibular bone defects.

scholarly journals Implantation of Autologous Stem Cells Derived from Adipose Tissue in Rat Bone Fractures

2018 ◽  
Vol 1 (2) ◽  
pp. 105-115
Author(s):  
Carolina Smok ◽  
Manuel Meruane ◽  
Mariana Rojas
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Bone Regeneration ◽  
Bone Fractures ◽  
Control Group ◽  
Vascular Density ◽  
Major Advance ◽  
Sprague Dawley ◽  
Angiogenic Growth Factors ◽  
Significant Difference

Stem cells derived from adipose tissue (ASCs) correspond to a major advance with respect to the bone regenerative medicine, as they have the ability for self-renewal, differentiation and paracrine stimulation to various types of tissues including bone and cartilage. The hypothesis of this study considers that fractures treated with ASCs, time decreases bone regeneration and vascularization increases, aiming to histologically evaluate bone regeneration and vascularization in these fractures. To accomplish this, 24 young male Sprague Dawley rats were used. The specimens were divided into two groups: Group A (treated) and group B (control). In both groups, the rats were euthanized at 11 and 21 days post-fracture. Statistically significant difference was observed in the number of newly formed trabeculae and vascular density in the treated group compared to control group concluded that rats treated with ASCs have a higher rate and better angiogenic bone regeneration, especially given the ability to synthesize components of the extracellular matrix of these cell, and the production of angiogenic growth factors.

scholarly journals Effects of Enhanced Hydrophilic Titanium Dioxide-Coated Hydroxyapatite on Bone Regeneration in Rabbit Calvarial Defects

2018 ◽  
Vol 19 (11) ◽  
pp. 3640 ◽  
Author(s):  
Ji-Eun Lee ◽  
Chung Bark ◽  
Hoang Quy ◽  
Seung-Jun Seo ◽  
Jae-Hong Lim ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Uv Irradiation ◽  
Early Stage ◽  
Healing Process ◽  
Negative Control ◽  
Control Group ◽  
Graft Material ◽  
Porous Hydroxyapatite ◽  
Significant Difference ◽  
Calvarial Defects

The regeneration of bone defects caused by periodontal disease or trauma is an important goal. Porous hydroxyapatite (HA) is an osteoconductive graft material. However, the hydrophobic properties of HA can be a disadvantage in the initial healing process. HA can be coated with TiO2 to improve its hydrophilicity, and ultraviolet irradiation (UV) can further increase the hydrophilicity by photofunctionalization. This study was designed to evaluate the effect of 5% TiO2-coated HA on rabbit calvarial defects and compare it with that of photofunctionalization on new bone in the early stage. The following four study groups were established, negative control, HA, TiO2-coated HA, and TiO2-coated HA with UV. The animals were sacrificed and the defects were assessed by radiography as well as histologic and histomorphometric analyses. At 2 and 8 weeks postoperatively, the TiO2-coated HA with UV group and TiO2-coated HA group showed significantly higher percentages of new bone than the control group (p < 0.05). UV irradiation increased the extent of new bone formation, and there was a significant difference between the TiO2-coated HA group and TiO2-coated HA with UV group. The combination of TiO2/HA and UV irradiation in bone regeneration appears to induce a favorable response.

Human Dental Pulp Stem Cells in Rat Mandibular Bone Defects

2019 ◽  
Vol 207 (3-4) ◽  
pp. 138-148 ◽  
Author(s):  
Rubia Teodoro Stuepp ◽  
Priscilla Barros Delben ◽  
Filipe Modolo ◽  
Andrea Gonçalves Trentin ◽  
Ricardo Castilho Garcez ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Formation ◽  
Dental Pulp ◽  
Bone Defects ◽  
Treated Group ◽  
Dental Pulp Stem Cells ◽  
Control Group ◽  
Mandibular Bone ◽  
Significant Difference ◽  
Human Dental Pulp

This study aimed to evaluate the use of human dental pulp stem cells (hDPSCs) in non-critical-sized mandibular bone defects in rats. hDPSCs from permanent teeth were isolated and engrafted in mandibular bone defects in rats for 7, 14, and 28 days; bone defects without cells formed the control group. Samples were evaluated by scanning electron microscopy (SEM), light microscopy (hematoxylin and eosin staining), and the regeneration area was measured by the Image J program. Before surgery procedures, the human dental pulp cells were characterized as dental pulp stem cells: fusiform morphology, plastic-adherent; expression of CD105, CD73, and CD90; lack of expression of CD45 and CD34, and differentiated into osteoblasts, adipocytes, and chondroblasts. The results indicated that within 7 days the control group presented a pronounced bone formation when compared with the treated group (p < 0.05). After 14 days, the treated group showed an increase in bone formation, but with no statistical difference among the groups (p > 0.05). In the final evaluated period there was no difference between the control group and the treated group (p > 0.05). There was a significant difference between 7 and 14 days (p < 0.05) and between 7 and 28 days (p < 0.05) in the treated group. In conclusion, there is no evidence that the use of hDPSCs in the conditions of this study could improve bone formation in non-critical-sized mandibular bone defects.

scholarly journals The combination of carbonate hydroxyapatite and human β-defensin 3 to enhance collagen fibre density in periodontitis Sprague Dawley rats

2020 ◽  
Vol 53 (2) ◽  
pp. 76
Author(s):  
Ika Andriani ◽  
Edy Meiyanto ◽  
S. Suryono ◽  
Ika Dewi Ana
Keyword(s):  
Bone Resorption ◽  
Alveolar Bone ◽  
Healing Process ◽  
Collagen Fibre ◽  
Control Group ◽  
Sprague Dawley ◽  
Carbonate Hydroxyapatite ◽  
Sprague Dawley Rats ◽  
Significant Difference ◽  
Collagen Density

Background: Carbonate hydroxyapatite (CHA) is used as a scaffold to repair bone resorption. Alveolar bone resorption in periodontitis caused by an infection requires the presence of an antibacterial to support bone regeneration. Human β-defensin 3 (HBD3) is an antimicrobial peptide. The local application of the HBD3 antimicrobial is beneficial to inhibiting drug resistance and protecting tissue regeneration against invasive bacteria. Purpose: This study aims to investigate the effect of the administration of a combination of   CHA with HBD3 on the collagen density of periodontitis rats (Sprague Dawley). Methods: This study was a true experimental study with a post-test control group design. Thirty-two Sprague Dawley animal models were randomly blind selected placed under anaesthetic, then a 2-mm silk ligature was attached as a ligation to the mandibular incisors for 14 days in order to generate periodontitis. The study subjects were divided into two groups, the group with CHA and CHA loaded HBD3 (CHA + HBD3) implantation. On days 7, 14, 21 and 28, four rats were taken randomly from each group for decapitation, followed by histological processing and examination with trichome Mallory staining. The data was analysed using the Kruskal Wallis test (p<0.05). Results: An increase in collagen density during the healing process was found. There was a significant difference between CHA and CHA+HBD (p=0.004 and p=0.008; p<0.05) in collagen density between the groups. Conclusion: The combination of CHA and HBD3 can enhance the collagen density in periodontitis Sprague Dawley rats, compared to CHA only groups.

scholarly journals Effects of Anadara granosa shell combined with Sardinella longiceps oil on oesteoblast proliferation in bone defect healing process

2016 ◽  
Vol 49 (1) ◽  
pp. 27
Author(s):  
Rima Parwati Sari ◽  
Eddy Hermanto ◽  
Dinda Divilia ◽  
Indra Candra ◽  
Wisnu Kuncoro ◽  
...  
Keyword(s):  
Alveolar Bone ◽  
Healing Process ◽  
Bone Defects ◽  
Male Rats ◽  
Control Group ◽  
Bone Defect Healing ◽  
Bone Damage ◽  
Significant Difference ◽  
Sardinella Longiceps ◽  
Anadara Granosa

Background: Alveolar bone damage is the most common case in dentistry. One way to fix the bone damage is by using bone graft. Anadara granosa shell is a potential bone substitute since it is rich in calcium which can be processed into hydroxyapatite. The addition of Sardinella longiceps oil rich in omega-3 can modulate inflammation, thus accelerating the healing process. Purpose: This study aimed to determine effects of application of Anadara granosa shell combined with Sardinella longiceps oil on osteoblast proliferation in the healing process of bone defects. Method: The subjects were 32 male rats type Wistar divided into 4 groups (n = 8). Making defect was performed on the right bone of the femurs with a half of the diameter of round Mcisinger® Germany bur sized 18. The first group (K) is a negative control group that was not given anything. The second group (AG) was given Anadara granosa pasta. The third group (AM10) was given Anadara granosa pasta combined with 10% Sardinella longiceps oil. And, the fourth group (AM30) was given Anadara granosa pasta combined with 30% Sardinella longiceps oil. Next, preparations and animal euthanasia were performed on the 7th day after the treatment. The number of osteoblasts then was measured after making preparations for HPA with Hematoxylin eosin staining (HE). Afterward, tabulation of data followed by statistical analysis of Anova and HSD Tukey was carried out. Result: The average number of osteoblasts in Groups K, AG, AM10, and AM30 was 19.00, 34.63, 33.50, and 38.50. The results of Anova test showed a significant difference (p<0.05). Similarly, the results of Tukey-HSD test also showed significant differences (p<0.05) between Group K and all other groups (AG, AM10, and AM30). Nevertheless, there were no significant differences between Group AG and Groups AM10 and AM30, as well as between Group AM10 and Group AM30. Conclusion: The application of the combination of Anadara granosa shell and Sardinella longiceps oil can not increase the proliferation of osteoblasts in the healing process of bone defects.

scholarly journals Expression of fibroblast growth factor-2 after application of the Queen's crepe-myrtle leaf (Lagerstroemia speciosa) and aloe vera extract gel in the wound healing process of hyperglycemic

2021 ◽  
Vol 33 (1) ◽  
pp. 48
Author(s):  
Fachrul Razi ◽  
Andri Hardianto ◽  
Lucky Riawan ◽  
Bambang Pontjo Priosoeryanto
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Aloe Vera ◽  
Simple Random Sampling ◽  
Fibroblast Growth Factor 2 ◽  
Control Group ◽  
Wound Healing Process ◽  
Sprague Dawley ◽  
Lagerstroemia Speciosa ◽  
Significant Difference

Introduction: Fibroblast Growth Factor-2 (FGF-2) have a role in stimulating the proliferation and migration of various types of cells in the wound healing process. Hyperglycemic conditions can disrupt the wound healing process. Lagerstroemia speciosa are known to have antyphyerglycemic and antioxidant effects. Aloe vera is a plant that has been used for long time in topical treatment of wounds.This study was aimed to analyse the FGF-2 expressions on the wound healing process of subjects with hyperglycemia after application of Queen's crepe-myrtle leaf (Lagerstroemia speciosa) and aloe vera extracts gel. Methods: A pure experimental study was conducted with simple random sampling. The sample was 27 experimental animals (CI 95%; power test 80%) of Sprague Dawley rat induced to have a hyperglycemic state using alloxan and had their palate injured. The immunohistochemical examination was performed to assess FGF-2 expression level. Samples were divided into three groups with a simple random sampling technique: nine of the rats received the Lagerstroemia speciosa extract gel application; nine rats received the aloe vera extract gel application on the injured palate; another nine rats in the control group. Examination of the FGF-2 expression level was performed on the third, seventh, and fourteenth observation days. Results: Overall, the control group had a significant difference with the Lagerstroemia speciosa and aloe vera extract gel group (p-value<0.05), however, the Lagerstroemia speciosa group did not have any significant difference with the aloe vera group (0.123>0.05). Conclusion: There was no difference in the amount of FGF-2 expressions on the wound healing process of the injured palatal tissue of Sprague-Dawley rats with hyperglycemic after application of the Lagerstroemia speciosa and aloe vera extract gel.

scholarly journals Evaluation of the Effectiveness of Esterified Hyaluronic Acid Fibers on Bone Regeneration in Rat Calvarial Defects

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Omer B. Agrali ◽  
Selin Yildirim ◽  
Hafize O. Ozener ◽  
Kemal N. Köse ◽  
Dilek Ozbeyli ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Bone Regeneration ◽  
Bone Defects ◽  
The Other ◽  
Bone Morphogenetic Protein 2 ◽  
Control Group ◽  
Collagen Membrane ◽  
Sprague Dawley ◽  
Calvarial Bone ◽  
Almost All

Hyaluronic acid (HA) constitutes one of the major components of the extracellular matrix domain in almost all mammals. The aim of this study was to evaluate the regenerative capacity of HA matrix in rat calvarial bone defects and compare with those of different combinations of resorbable collagen membrane (M) and bovine-derived xenograft (G). Twenty-four 3-month-old male Sprague-Dawley rats weighing 200-250 g were included. Control group was created by leaving one defect empty from 2 critical size defects with 5 mm diameter formed in the calvarial bones of 8 rats. In the same rats, the other defect was treated with HA matrix alone. One of the 2 defects formed in other 8 rats was treated with HA+G and the other with HA+M. One of the 2 defects formed in the remaining 8 rats was treated with G+M and the other with HA+G+M. The animals were sacrificed at 4 weeks. Histologic, histomorphometric, and immunohistochemical analyses were performed. Both HA matrix alone and its combinations with G and M supported new bone formation (NBF). However, NBF was significantly greater in G+M and HA+G+M groups compared to control and HA alone (P<0.001). Bone morphogenetic protein-2 was expressed with varying degrees in all groups, without any difference among them. Within the limitations of the present study, HA matrix, used alone or in combination with G and M, did not contribute significantly to bone regeneration in rat calvarial bone defects.

scholarly journals Kombinasi Suplementasi Omega 3 Dengan Restriksi Kalori Terhadap Penyembuhan Luka Bakar Tikus Sprague Dawley Kajian Pada VEGF dan Re-Epitelisasi

2020 ◽  
Vol 7 (2) ◽  
pp. 432-438
Author(s):  
Doglas Rama Bayu ardha ◽  
Edi Dharmana ◽  
Erik Prabowo
Keyword(s):  
Calorie Restriction ◽  
Cell Injury ◽  
Healing Process ◽  
Control Group ◽  
P Value ◽  
Omega 3 ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Significant Difference ◽  
One Way Anova

Cedera sel yang luas pada luka bakar melepaskan banyak mediator inflamasi. Respon tubuh yang berlebihan pada keadaan tersebut akan memperberat kerusakan sel yang sudah terjadi. Nurtrisi (lemak) yang cukup seperti omega 3 dapat membantu proses penyembuhan luka serta mencegah infeksi pada luka supaya tidak menjadi luka kronis. Maka dari itu peneliti memilih judul penelitian tentang efektifitas kombinasi suplementasi omega 3 dengan restriksi kalori terhadap penyembuhan luka bakar pada tikus Sprague Dawley, ditinjau dari VEGF dan Re-epitelisasi. Untuk mengetahui tingkat efektifitas suplementasi Omega 3 dan retriksi kalori pada penyembuhan luka bakar. Peneliti dalam melakukan penelitian menggunakan metode penelitian eksperimental dengan menggunakan 1 kelas kontrol dan 3 kelas perlakuan tikus Sprague Dawley, yang diinduksi luka bakar kemudian diberi perlakuan, dan diamati secara berkala. Hasil penelitian didapatkan perbedaan yang signifikan antara kelompok kontrol dan kelompok perlakuan yang di uji dengan one way ANOVA mempunyai nilai p = 0,000 (p < 0,05), dan uji Mann-Whitney didapatkan perbedaan yang signifikan antara kelompok perlakuan 1, 2, 3, dan kelompok kontrol p = 0,025; 0,004; 0,004; 0,004; 0,004, nilai p < 0,05. Dan uji Games-Howell ditunjukkan pada kolom rerata ± SD jika memuat huruf yang  berbeda berarti ada perbedaan yang bermakna (p-value <0.05) K1 = 23,3587a ± 0,57556; P1 = 17,1015b ± 2,60108; P2 = 124,1503c ± 15,30168; P3 = 237,5053d ± 0,78263. Kesimpulan yang peneliti dapat bahwa kombinasi suplementasi omega 3 dengan retriksi kalori berpengaruh signifikan terhadap penyembuhan luka bakar pada tikus Sprague Dawley. Kata kunci : Penyembuhan Luka bakar, Sprague Dawley, Omega 3, Retriksi Kalori   Extensive cell injury to burns releases inflammatory mediators. It’s led to excessive body response and cause aggravate cell damage that has already occurred. Adequate nutrients (fat) like omega 3 can help the healing process and prevent infection in wounds to become chronic. Therefore the researchers chose the title of the study of the effectiveness combination omega-3 supplementation with calorie restriction for burns in Sprague Dawley rats, in terms of VEGF and Re-epithelialization. To determine the effectiveness of Omega 3 supplementation and calorie restriction when healing burns. The researchers using experimental research methods with 1 control and 3 training classes of Sprague Dawley mice, which were induced by burns were then observed periodically. The results showed a significant difference between the control group and the treatment group tested by one way ANOVA having a value of p = 0,000 (p <0.05), and the Mann-Whitney test found a significant difference between the treatment groups 1, 2, 3, and the control group p = 0.025; 0.004; 0.004; 0.004; 0.004, p value <0.05. And the Games-Howell test is shown in the mean column ± SD if it contains different letters it means there is a significant difference (p-value <0.05) K1 = 23.3587a ± 0.57556; P1 = 17.1015b ± 2.60108; P2 = 124,1503c ± 15.30168; P3 = 237.5053 d ± 0.78263. The researchers concluded that the combination of omega-3 supplementation with calorie retention had a significant effect on burn healing in Sprague Dawley rats. Keywords: Burn Healing, Sprague Dawley, Omega 3, Calorie Restriction

scholarly journals The Effect of Single and Triple Testicular Biopsy Using Biopty Gun on Spermatogenesis in Pubertal Rats

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1569
Author(s):  
Tomislav Šušnjar ◽  
Ivana Kuzmić Prusac ◽  
Ivan Švagelj ◽  
Anđela Jurišić ◽  
Tomislav Šušnjar ◽  
...  
Keyword(s):  
Sperm Count ◽  
Semen Analysis ◽  
Study Groups ◽  
Testicular Biopsy ◽  
Male Rats ◽  
Lower Percentage ◽  
Control Group ◽  
Sprague Dawley ◽  
Left Testis ◽  
Significant Difference

Background: The aim of this study was to compare consequences in single and triple testicular biopsy by biopty gun in pubertal rats using histological and immunohistochemical analysis. Methods: Thirty-two Sprague-Dawley male rats were used as the experimental model. The rats were randomly divided into three study groups. The rats from the first group (n = 12) received a single-biopsy of upper pole of the left testis, while the rats from the second group (n = 10) received triple-biopsy of upper and lower poles and lateral surface of left testis. The third group (n = 10) was a control group. On the eightieth day after the biopsy in all rats bilateral orchiectomy and funiculectomy were performed to obtain testicular tissue and sperm for analysis. The consequences of the puncture were observed by pathohistology, immunohistochemistry and semen analysis. Results: The results of the study showed lower percentage of sperm count (14.5 mill/mL vs. 16 mill/mL, p = 0.130), sperm motility (24.6% vs. 32.7%, p > 0.05), abnormal sperm (30% vs. 27%, p > 0.05), atrophic tubules (21% vs. 6%, p < 0.001), volume (1.7 mL vs. 2.28 mL, p < 0.01) and apoptotic index (1.56 vs. 1.19, p = 0.650) in the testes with a triple-biopsy compared to the testes with a single-biopsy. Semen analysis showed a borderline significant difference between the group with triple-biopsy where sperm count was lower than it in the control group (14.5 mill/mL vs. 17.5 mill/mL, p = 0.05). A single-biopsy has little effect on the testis, especially on overall fertility. A triple-biopsy showed higher degree of the testicular damage but without a significant impact on overall fertility. Semen analysis showed that single- and triple-biopsies did not have a significant effect on sperm count, motility and morphology. Conclusion: Biopty gun procedure is a cheap, simple and reliable method for testicular biopsy in rats without a significant effect on sperm count, motility and morphology.

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