Non-propagating, recombinant vesicular stomatitis virus vectors encoding respiratory syncytial virus proteins generate potent humoral and cellular immunity against RSV and are protective in mice

2013 ◽  
Vol 150 (1-2) ◽  
pp. 134-144 ◽  
Author(s):  
J. Erik Johnson ◽  
Lisa K. McNeil ◽  
Shakuntala Megati ◽  
Susan E. Witko ◽  
Vidia S. Roopchand ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Vesicular Stomatitis Virus ◽  
Cellular Immunity ◽  
Virus Vectors ◽  
Humoral And Cellular Immunity ◽  
Vesicular Stomatitis ◽  
Syncytial Virus ◽  
Virus Proteins

scholarly journals Valosin-Containing Protein (VCP/p97) Is a Potential Antiviral Target against Mononegavirales

Proceedings ◽  
2020 ◽  
Vol 50 (1) ◽  
pp. 108
Author(s):  
Victor Latorre ◽  
Ron Geller
Keyword(s):  
Life Cycle ◽  
Respiratory Syncytial Virus ◽  
Vesicular Stomatitis Virus ◽  
Molecular Chaperones ◽  
Cellular Machinery ◽  
Vesicular Stomatitis ◽  
Medical Importance ◽  
Syncytial Virus ◽  
Chaperone Network

The viral order Mononegavirales consist of eight virus families. Members of these families include some of the most infectious (Measles, lethal (Ebola and Rabies), and most common viruses (Respiratory syncytial virus, RSV). Despite their medical importance, few vaccines and no antiviral treatments are available for treating infections with these viruses. Being obligate cellular parasites, viruses must rely on the cellular machinery for their replication. One example of this is the widespread use of molecular chaperones, which assist the correct folding of newly synthesized proteins, refold misfolded or aggregated proteins, and play key roles in maintaining proteostasis in cells. Targeting chaperones required for viral replication may, therefore, provide an antiviral approach. In this work, we set out to identify all the members of the cytoplasmic chaperone network that are involved in the replication of RSV using an RNA interference screen. Among our hits is valosin-containing protein (VCP; also known as p97), a chaperone involved in ubiquitin-mediated protein degradation, which has been shown to play a role in the life cycle of several viruses. We investigated the role of VCP during RSV and vesicular stomatitis virus (VSV) infections using specific VCP inhibitors. Our results suggest that VCP activity is necessary for RSV and VSV replication and may constitute a promising antiviral approach for the Mononegavirales.

scholarly journals Agmatine-Containing Poly(amidoamine)s as a Novel Class of Antiviral Macromolecules: Structural Properties andIn VitroEvaluation of Infectivity Inhibition

2014 ◽  
Vol 58 (10) ◽  
pp. 6315-6319 ◽  
Author(s):  
Manuela Donalisio ◽  
Elisabetta Ranucci ◽  
Valeria Cagno ◽  
Andrea Civra ◽  
Amedea Manfredi ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Herpes Simplex ◽  
Antiviral Activity ◽  
Vesicular Stomatitis Virus ◽  
Human Papillomavirus 16 ◽  
Human Rotavirus ◽  
Vesicular Stomatitis ◽  
Syncytial Virus ◽  
Simplex Virus ◽  
Structural Versatility

ABSTRACTPoly(amidoamine)s (PAAs) are multifunctionaltert-amine polymers endowed with high structural versatility. Here we report on the screening of a minilibrary of PAAs against a panel of viruses. The PAA AGMA1 showed antiviral activity against herpes simplex virus, human cytomegalovirus, human papillomavirus 16, and respiratory syncytial virus but not against human rotavirus and vesicular stomatitis virus. The results suggest the contribution of both a polycationic nature and side guanidine groups in imparting antiviral activity.

scholarly journals A Novel Respiratory Syncytial Virus (RSV) F Subunit Vaccine Adjuvanted with GLA-SE Elicits Robust Protective TH1-Type Humoral and Cellular Immunity In Rodent Models

PLoS ONE ◽  
2015 ◽  
Vol 10 (3) ◽  
pp. e0119509 ◽  
Author(s):  
Stacie L. Lambert ◽  
Shahin Aslam ◽  
Elizabeth Stillman ◽  
Mia MacPhail ◽  
Christine Nelson ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Cellular Immunity ◽  
Subunit Vaccine ◽  
Rodent Models ◽  
Humoral And Cellular Immunity ◽  
Syncytial Virus
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