Resveratrol augments therapeutic efficiency of mouse bone marrow mesenchymal stem cell‐based therapy in experimental autoimmune encephalomyelitis

2016 ◽  
Vol 49 (1) ◽  
pp. 60-66 ◽  
Author(s):  
Dong Wang ◽  
Shi‐Ping Li ◽  
Jin‐Sheng Fu ◽  
Lin Bai ◽  
Li Guo
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Experimental Autoimmune Encephalomyelitis ◽  
Mouse Bone Marrow ◽  
Autoimmune Encephalomyelitis ◽  
Therapeutic Efficiency ◽  
Cell Based Therapy ◽  
Experimental Autoimmune

Mature bone marrow-derived dendritic cells polarize Th2 response and suppress experimental autoimmune encephalomyelitis

2002 ◽  
Vol 8 (6) ◽  
pp. 463-468 ◽  
Author(s):  
G-X Zhang ◽  
M Kishi ◽  
H Xu ◽  
A Rostami
Keyword(s):  
Bone Marrow ◽  
Dendritic Cells ◽  
Experimental Autoimmune Encephalomyelitis ◽  
Mhc Class Ii ◽  
Clinical Signs ◽  
Mouse Bone Marrow ◽  
Autoimmune Encephalomyelitis ◽  
Th2 Response ◽  
Gm Csf ◽  
Experimental Autoimmune

Distinct subsets of dendritic cells (DCs) based on the origin, phenotypes, and the nature of the signals that promote DC maturation can determine polarized immune responses of T cells. In this study, DCs were cultured from mouse bone marrow (BM) progenitors in granulocyte-macrophage colony-stimulating factor (GM-CSF). To generate mature DCs (mDCs), lipopolysaccharide (LPS) was used in the culture for 24 h. LPS-stimulated DCs were phenotypically mature, which exhibited strongly upregulated CD40, B7.1, and B7.2 compared to non-LPS-stimulated immature DCs (imDCs). Both mDCs and imDCs expressed high levels of MHC class II but low level of CD54. mDCs produced higher levels of IL-10 and lower IL-12 compared to imDCs. No IFN-g or IL-4 was found in both groups. When mDCs were injected intraperitoneally (i.p.) to the mice with experimental autoimmune encephalomyelitis (EAE), the severity of clinical signs and inflammation in the CNS was significantly suppressed compared to imDC-injected mice (p<0.01) and PBS-injected mice (p <0.02). Moreover, lymphocytes from mDC-injected mice produced lower level of IL-12, IFN-g, but higher level of IL-10, compared to imDC-injected and non-DC-injected mice. We conclude that BM-mDCs, but not BM-imDCs, promote Th2 differentiation and have the potential for suppression of inflammatory demyelination.

scholarly journals Engineered stem cell-derived microglia as therapeutic vehicle for experimental autoimmune encephalomyelitis

Gene Therapy ◽  
2013 ◽  
Vol 20 (8) ◽  
pp. 797-806 ◽  
Author(s):  
C Beutner ◽  
V Lepperhof ◽  
A Dann ◽  
B Linnartz-Gerlach ◽  
S Litwak ◽  
...  
Keyword(s):  
Stem Cell ◽  
Experimental Autoimmune Encephalomyelitis ◽  
Autoimmune Encephalomyelitis ◽  
Experimental Autoimmune
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