Application of response surface methodology for optimization of important parameters in decolorizing treated distillery wastewater using Aspergillus fumigatus UB2 60

2006 ◽  
Vol 57 (4) ◽  
pp. 195-199 ◽  
Author(s):  
Pazouki Mohammad ◽  
Hussainnia Azarmidokht ◽  
Moztarzadeh Fatollah ◽  
Banifathemi Mahboubeh
Keyword(s):  
Response Surface Methodology ◽  
Aspergillus Fumigatus ◽  
Response Surface ◽  
Distillery Wastewater

scholarly journals Cultivating conditions optimization of the anaerobic digestion of corn ethanol distillery residuals using response surface methodology

2014 ◽  
Vol 12 (8) ◽  
pp. 868-876 ◽  
Author(s):  
László Gyenge ◽  
Botond Ráduly ◽  
Silvia Crognale ◽  
Szabolcs Lányi ◽  
Beáta Ábrahám
Keyword(s):  
Response Surface Methodology ◽  
Anaerobic Digestion ◽  
Response Surface ◽  
Fold Increase ◽  
Interactive Effects ◽  
Corn Ethanol ◽  
Qualitative Variable ◽  
Distillery Wastewater ◽  
The Individual ◽  
Inoculum Type

AbstractThis study investigated the individual and interactive effects of three factors — temperature, inoculum/substrate ratio (ISR) and inoculum typology — on the anaerobic digestion of corn ethanol distillery wastewater. Biochemical methane potential assays planned with factorial design with two independent quantitative variables on three levels (ISR: 1:1, 2:1 and 3:1; temperature: 30°C, 33.5°C, 37°C) and one independent qualitative variable (inoculum type: suspended, granular, mixed) have been performed. Response Surface Methodology has been used to study the effect of the factors with the aim of maximizing the specific methane yields (YCH4) obtainable with this substrate. The results show that all three investigated factors influence in a significant matter the YCH4, the ISR having the strongest effect on it. The temperature has significant influence on the YCH4 only in combination with high ISR values. The optimal conditions for the maximum YCH4 (551 mL CH4 g−1 VSadded) have been found at 37°C operating temperature, ISR=3:1 and using granular inoculum. These conditions gave rise to a 4-fold increase of YCH4 with respect to the worst combination of factors (YCH4=129 mL g−1 VSadded for the suspended inoculum type, at 30°C and ISR=1:1). The results improve the knowledge on the digestion of this substrate, providing information for successful process up-scaling.

scholarly journals Implementation of Response Surface Methodology for Enhanced Production of Endoglucanase by Thermophilic Aspergillus Fumigatus

2021 ◽  
Vol 3 (4) ◽  
Author(s):  
Ansar Ahmed Abbasi ◽  
Raja Tahir Mahmood ◽  
Sufia Tazeen ◽  
Muhammad Javaid Asad ◽  
Mudassar Zafar Zafar ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Enzyme Activity ◽  
Aspergillus Fumigatus ◽  
Response Surface ◽  
Plant Biomass ◽  
Gel Filtration ◽  
Industrial Applications ◽  
Inoculum Size ◽  
The Body ◽  
Enhanced Production

Enzymes are biocatalysts which play key roles in the body of living organisms. Cellulose is major source of plant biomass. Its β-1,4-glucosidic bonds  are hydrolyzed by cellulases. These cellulases can be produced by a variety of microorganisms including fungi, bacteria and actinomycetes which are used today for the industrial applications. The current study was aimed to optimize the cultural conditions for maximum production of endoglucanase by Aspergillus fumigatus through solid state fermentation of sugarcane bagasse. Response Surface Methodology (RSM) was employed under Central Composite Design (CCD) for the optimization of growth including pH, temperature, time period & inoculum size and nutritional parameters including glucose, fructose and (NH₄)₂SO₄. The effect of different metal ions on endoglucanase production was also monitored. It was partially purified by (NH₄)₂SO₄ precipitation and gel filtration chromatography. Finally, edogucanase was characterized for optimum pH, temperature and determination of kinetic parameters. Maximum enzyme activity was found as 0.9 IU/mL/min in the presence of 6 g substrate, 3.5 mL inoculum, 4.5 pH, 40 °C temperature at incubation time of 84 hrs. After addition of carbon and nitrogen sources enzyme activity increased to 1.4 IU/mL/min. It was further increased to 1.56 IU/mL/min with 0.42% of CaCl2. Maximum purification was achieved at 50% saturation by ammonium sulphate (NH₄)₂SO₄. Optimum temperature and pH were 40 °C and 5 respectively, whereas the values for Km and Vmax were 5.37 mM and 696 uM/mL/min., respectively. These findings suggested that endoglucanase by Aspergillus fumigatus could be suitable for various industries. Copyright (c) The Authors

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