Bioprocess optimization using response surface methodology for production of the anticancer drug paclitaxel by Aspergillus fumigatus and Alternaria tenuissima: Enhanced production by ultraviolet and gamma irradiation

2019 ◽  
Vol 18 ◽  
pp. 100996 ◽  
Author(s):  
El-Sayed R. El-Sayed ◽  
Ahmed A. Ismaiel ◽  
Ashraf S. Ahmed ◽  
Ismail A. Hassan ◽  
Al-Zahraa A. Karam El-Din
Keyword(s):  
Response Surface Methodology ◽  
Gamma Irradiation ◽  
Aspergillus Fumigatus ◽  
Response Surface ◽  
Anticancer Drug ◽  
Bioprocess Optimization ◽  
Alternaria Tenuissima ◽  
Enhanced Production

scholarly journals Implementation of Response Surface Methodology for Enhanced Production of Endoglucanase by Thermophilic Aspergillus Fumigatus

2021 ◽  
Vol 3 (4) ◽  
Author(s):  
Ansar Ahmed Abbasi ◽  
Raja Tahir Mahmood ◽  
Sufia Tazeen ◽  
Muhammad Javaid Asad ◽  
Mudassar Zafar Zafar ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Enzyme Activity ◽  
Aspergillus Fumigatus ◽  
Response Surface ◽  
Plant Biomass ◽  
Gel Filtration ◽  
Industrial Applications ◽  
Inoculum Size ◽  
The Body ◽  
Enhanced Production

Enzymes are biocatalysts which play key roles in the body of living organisms. Cellulose is major source of plant biomass. Its β-1,4-glucosidic bonds  are hydrolyzed by cellulases. These cellulases can be produced by a variety of microorganisms including fungi, bacteria and actinomycetes which are used today for the industrial applications. The current study was aimed to optimize the cultural conditions for maximum production of endoglucanase by Aspergillus fumigatus through solid state fermentation of sugarcane bagasse. Response Surface Methodology (RSM) was employed under Central Composite Design (CCD) for the optimization of growth including pH, temperature, time period & inoculum size and nutritional parameters including glucose, fructose and (NH₄)₂SO₄. The effect of different metal ions on endoglucanase production was also monitored. It was partially purified by (NH₄)₂SO₄ precipitation and gel filtration chromatography. Finally, edogucanase was characterized for optimum pH, temperature and determination of kinetic parameters. Maximum enzyme activity was found as 0.9 IU/mL/min in the presence of 6 g substrate, 3.5 mL inoculum, 4.5 pH, 40 °C temperature at incubation time of 84 hrs. After addition of carbon and nitrogen sources enzyme activity increased to 1.4 IU/mL/min. It was further increased to 1.56 IU/mL/min with 0.42% of CaCl2. Maximum purification was achieved at 50% saturation by ammonium sulphate (NH₄)₂SO₄. Optimum temperature and pH were 40 °C and 5 respectively, whereas the values for Km and Vmax were 5.37 mM and 696 uM/mL/min., respectively. These findings suggested that endoglucanase by Aspergillus fumigatus could be suitable for various industries. Copyright (c) The Authors

Strain improvement and immobilization technique for enhanced production of the anticancer drug paclitaxel by Aspergillus fumigatus and Alternaria tenuissima

2019 ◽  
Vol 103 (21-22) ◽  
pp. 8923-8935 ◽  
Author(s):  
El-Sayed R. El-Sayed ◽  
Ashraf S. Ahmed ◽  
Ismail A. Hassan ◽  
Ahmed A. Ismaiel ◽  
Al-Zahraa A. Karam El-Din
Keyword(s):  
Aspergillus Fumigatus ◽  
Anticancer Drug ◽  
Strain Improvement ◽  
Alternaria Tenuissima ◽  
Enhanced Production

scholarly journals Novel Sequential Screening and Enhanced Production of Fibrinolytic Enzyme byBacillussp. IND12 Using Response Surface Methodology in Solid-State Fermentation

2017 ◽  
Vol 2017 ◽  
pp. 1-13 ◽  
Author(s):  
Ponnuswamy Vijayaraghavan ◽  
P. Rajendran ◽  
Samuel Gnana Prakash Vincent ◽  
Arumugaperumal Arun ◽  
Naif Abdullah Al-Dhabi ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Solid State ◽  
Response Surface ◽  
Solid State Fermentation ◽  
Enzyme Production ◽  
Blood Clot ◽  
Fibrinolytic Enzyme ◽  
Surface Model ◽  
Cow Dung ◽  
Enhanced Production

Fibrinolytic enzymes have wide applications in clinical and waste treatment. Bacterial isolates were screened for fibrinolytic enzyme producing ability by skimmed milk agar plate using bromocresol green dye, fibrin plate method, zymography analysis, and goat blood clot lysis. After these sequential screenings,Bacillussp. IND12 was selected for fibrinolytic enzyme production.Bacillussp. IND12 effectively used cow dung for its growth and enzyme production (687±6.5 U/g substrate). Further, the optimum bioprocess parameters were found out for maximum fibrinolytic enzyme production using cow dung as a low cost substrate under solid-state fermentation. Two-level full-factorial experiments revealed that moisture, pH, sucrose, peptone, and MgSO4were the vital parameters with statistical significance (p<0.001). Three factors (moisture, sucrose, and MgSO4) were further studied through experiments of central composite rotational design and response surface methodology. Enzyme production of optimized medium showed4143±12.31 U/g material, which was more than fourfold the initial enzyme production (978±36.4 U/g). The analysis of variance showed that the developed response surface model was highly significant (p<0.001). The fibrinolytic enzyme digested goat blood clot (100%), chicken skin (83±3.6%), egg white (100%), and bovine serum albumin (29±4.9%).

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