In vitro germination and growth of lily pollen tubes is affected by calcium inhibitor with reference to calcium distribution

Flora ◽  
2007 ◽  
Vol 202 (7) ◽  
pp. 581-588 ◽  
Author(s):  
Jingmei Zhang ◽  
Jiaxi Liu ◽  
Zukeng Chen ◽  
Jinxing Lin
Keyword(s):  
Pollen Tubes ◽  
In Vitro Germination ◽  
Calcium Distribution ◽  
Lily Pollen ◽  
Germination And Growth

In-vitro germination and growth of lily pollen tubes is affected by protein phosphatase inhibitors

Planta ◽  
1998 ◽  
Vol 207 (2) ◽  
pp. 303-312 ◽  
Author(s):  
Gerhard Obermeyer ◽  
Hildegard Klaushofer ◽  
Marion Nagl ◽  
Margit Höftberger ◽  
Friedrich-Wilhelm Bentrup
Keyword(s):  
Protein Phosphatase ◽  
Pollen Tubes ◽  
In Vitro Germination ◽  
Phosphatase Inhibitors ◽  
Lily Pollen ◽  
Protein Phosphatase Inhibitors ◽  
Germination And Growth

scholarly journals Germination and growth of ornamental pepper plants due to salinity

2018 ◽  
Vol 39 (1) ◽  
pp. 61
Author(s):  
Angela Maria dos Santos Pessoa ◽  
Elizanilda Ramalho do Rêgo ◽  
Marcelo Pereira Cruz ◽  
Cristine Agrine Pereira dos Santos ◽  
Priscila Duarte Silva ◽  
...  
Keyword(s):  
Capsicum Annuum ◽  
Leaf Length ◽  
Leaf Width ◽  
Seedling Height ◽  
In Vitro Germination ◽  
Fresh Matter ◽  
Cotyledonary Leaf ◽  
Germination And Growth ◽  
Descriptive Statistical Analysis

Seeds need favorable environmental conditions to germinate but sometimes these conditions are not available. The goal of this work was to evaluate the influence of different concentrations of NaCl on in vitro germination and development of Capsicum annuum seedlings. The experiment was conducted at the Laboratório de Biotecnologia Vegetal at Centro de Ciências Agrárias (CCA) of Universidade Federal da Paraíba (UFPB), Areia, PB, Brazil. Ornamental pepper seeds (Capsicum annuum L.) were submitted to five concentrations of NaCl: 0, 25, 50, 75 and 100 mM. The experimental design was completely randomized, with five treatments and seven replicates, each replicate consisted of one flask with one seed. After 14 days in vitro, the germinated seedlings were characterized for seedling height, hypocotyl width, cotyledon leaf length, cotyledon leaf width, leaf number, radicle length, fresh matter and germination. The data were submitted to analysis of variance by the F test ( p ≤ 0.05). When significant differences were detected, the data were submitted to regression analysis. Percentage of abnormal plantlets was also calculated and submitted to descriptive statistical analysis. The percentage of germination was not affected by the NaCl concentrations used in this work. However 75 e 100 mM salt concentrations influenced the cotyledonary leaf width, total fresh matter and percentage of abnormal seedlings.

scholarly journals In vitro germination and growth of Vriesea incurvata Gaudich. (Bromeliaceae)

2018 ◽  
Vol 67 (1) ◽  
pp. 142-147 ◽  
Author(s):  
Edwin Ernesto Pulido-Rueda ◽  
Maria Auxiliadora Milaneze-Gutierre ◽  
Raquel Negrelle
Keyword(s):  
In Vitro Germination ◽  
Germination And Growth

Vriesea incurvata en el ambiente natural presenta algunas dificultades asociadas a la baja capacidad de germinación de semillas. Con el objetivo de proporcionar una base para la producción de plántulas, se reportan los resultados de la evaluación del porcentaje de germinación de semillas y crecimiento inicial de plántulas de V. incurvata, en condiciones in vitro en diferentes medios de cultivo. El diseño fue completamente al azar con ocho tratamientos y ocho repeticiones. Los tratamientos fueron los medios de cultivo: MS (Murashige & Skoog); MS½; KC (Knudson); KC½; MS + carbón activado (CA); MS½ + CA; KC + CA; y KC½ + CA. El porcentaje de germinación fue calculado a partir de la división entre el número de semillas con extrusión de la raíz primaria por el número total de semillas sembradas. El crecimiento inicial fue evaluado considerando los valores de biomasa fresca total, porcentaje de plántulas normales y muertas, número y longitud de raíces, longitud del tallo, número de hojas y porcentaje de hojas cloróticas, necróticas y muertas. Todos los medios de cultivo promovieron altos porcentajes de germinación (>82,8%). En los medios de cultivo MS y MS½ se evidenció el mayor porcentaje de plántulas normales, mayores valores de producción de biomasa fresca, crecimiento del tallo y número de hojas. Los medios de cultivo KC y KC½ también promovieron mayores porcentajes de plántulas normales y bajos porcentajes de hojas necróticas y muertas. La adición de carbón activado en los medios de cultivo fue desfavorable en la promoción del crecimiento de las plántulas. Se concluye que MS y MS½ son los medios de cultivo más adecuados para la producción in vitro de Vriesea incurvata.

GERMINATION OF SWEET CHERRY (PRUNUS AVIUM L.) POLLEN IN VITRO AS INFLUENCED BY FUNGICIDES

1961 ◽  
Vol 41 (4) ◽  
pp. 740-743 ◽  
Author(s):  
G. W. Eaton
Keyword(s):  
Pollen Germination ◽  
Sweet Cherry ◽  
Prunus Avium ◽  
Pollen Tubes ◽  
Germination Of Pollen ◽  
Germination And Growth ◽  
Prunus Avium L

Effects of fungicidal concentrations of sulphur, dichlone, ferbam, and captan on sweet cherry (Prunus avium L.) pollen germination and growth were studied in artificial media. Sulphur did not reduce the germination of pollen. Dichlone and ferbam reduced germination from 53.0 per cent to 47.1 per cent and 40 per cent, respectively, reductions which were significant statistically. Sprayed at 0.2 pound/100 gallons or less, captan did not reduce germination. Captan, sprayed at 2 pounds/100 gallons, almost entirely prevented pollen germination, and arrested the elongation of pollen tubes.

Partial purification of myosin from lily pollen tubes by monitoring with in vitro motility assay

PROTOPLASMA ◽  
1992 ◽  
Vol 170 (1-2) ◽  
pp. 77-85 ◽  
Author(s):  
T. Kohno ◽  
R. Ishikawa ◽  
T. Nagata ◽  
K. Kohama ◽  
T. Shimmen
Keyword(s):  
Pollen Tubes ◽  
Motility Assay ◽  
Partial Purification ◽  
In Vitro Motility Assay ◽  
In Vitro Motility ◽  
Lily Pollen

scholarly journals Unequal Growth Rate of Pollen Tubes from Normal and Stringless Pea Genotypes

HortScience ◽  
1992 ◽  
Vol 27 (7) ◽  
pp. 833-834 ◽  
Author(s):  
Rebecca J. McGee ◽  
James R. Baggett
Keyword(s):  
Growth Rate ◽  
Pisum Sativum ◽  
Pollen Tube ◽  
Time Course ◽  
Pollen Tubes ◽  
In Vitro Germination ◽  
Pollen Tube Growth Rate ◽  
Germination Of Pollen

There was no difference in percentage in vitro germination of pollen from stringless pea (Pisum sativum L.) cv. Sugar Daddy and stringy `Oregon Sugarpod II' (OSP) and `OSU 705' (705). However, pollen tubes of `Sugar Daddy' grew more slowly in vitro than those of OSP or 705. Differences in pollen tube growth rate were demonstrated in vivo following time-course pollinations involving reciprocal crosses of `Sugar Daddy' with OSP and 705, along with the selfed parents. After 8 hours, pollen tubes from stringless peas (“stringless” pollen) had entered 13% of the ovules compared with 51% for those from stringy peas (“stringy” pollen). Stringless pollen tubes entered 29% and stringy pollen tubes 66% of the ovules after 10 hours. The slower growth of stringless compared with stringy pollen tubes is a plausible explanation for previously observed deficiencies of stringless plants in segregating populations.

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