Natural occurrence of moulds and aflatoxin B1 in melon seeds from markets in Nigeria

2004 ◽  
Vol 42 (8) ◽  
pp. 1309-1314 ◽  
Author(s):  
S.A Bankole ◽  
B.M Ogunsanwo ◽  
O.O Mabekoje
Keyword(s):  
Aflatoxin B1 ◽  
Natural Occurrence ◽  
Melon Seeds

Natural occurrence of aflatoxin B1 in some Indonesian food and feed products in Yogyakarta in year 1998–1999

2001 ◽  
Vol 17 (S2) ◽  
pp. 174-177 ◽  
Author(s):  
CT Noviandi ◽  
E Razzazi ◽  
A Agus ◽  
J Böhm ◽  
HW Hulan ◽  
...  
Keyword(s):  
Aflatoxin B1 ◽  
Natural Occurrence ◽  
Food And Feed

Fungal contamination and aflatoxin B1 of ‘egusi’ melon seeds in Nigeria

Food Control ◽  
2006 ◽  
Vol 17 (10) ◽  
pp. 814-818 ◽  
Author(s):  
S.A. Bankole ◽  
B.M. Ogunsanwo ◽  
A. Osho ◽  
G.O. Adewuyi
Keyword(s):  
Aflatoxin B1 ◽  
Fungal Contamination ◽  
Melon Seeds

scholarly journals Toxigenic fungal and mycotoxin contamination of maize samples from different districts in Serbia

2018 ◽  
Vol 34 (2) ◽  
pp. 239-249 ◽  
Author(s):  
Vesna Krnjaja ◽  
Slavica Stankovic ◽  
Milos Lukic ◽  
Nenad Micic ◽  
Tanja Petrovic ◽  
...  
Keyword(s):  
Aflatoxin B1 ◽  
Animal Feed ◽  
Morphological Characteristics ◽  
Common Species ◽  
Fungal Species ◽  
Aspergillus Species ◽  
Natural Occurrence ◽  
Mycotoxin Contamination ◽  
Toxigenic Fungi ◽  
Penicillium Spp

This study was carried out in order to investigate the natural occurrence of toxigenic fungi and levels of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in the maize stored immediately after harvesting in 2016 and used for animal feed in Serbia. A total of 22 maize samples were collected from four different districts across the country: City of Belgrade (nine samples), Sumadija (eight samples), Podunavlje (four samples) and Kolubara (one sample). Toxigenic fungi were identified according to the morphological characteristics whereas the mycotoxins contamination were detected using biochemistry enzyme-linked immuno-sorbent (ELISA) assay. The tested samples were mostly infected with Aspergillus, Fusarium and Penicillium spp., except that one sample originated from Kolubara was not contaminated with Aspergillus species. Fusarium graminearum was the most common species in the maize sample from Kolubara district (60%), F. verticillioides in the maize samples from Podunavlje (43.75%) and City of Belgrade (22.4%) districts, and Penicillium spp. in the maize samples from Sumadija district (26.38%). In the analysed maize samples the presence of Aspergillus species was low (0-1.78%). Mycotoxicological analysis revealed the presence of zearalenone (ZEA), deoxynivalenol (DON) and aflatoxin B1 (AFB1) in all the investigated samples, except that DON and AFB1 were not recorded in the samples from Podunavlje and Kolubara districts, respectively. The investigated samples were highly contaminated with ZEA, with incidence of 100% for the samples from Sumadija, Podunavlje and Kolubara districts and 88.89% for the samples from City of Belgrade district. In addition, the samples contamination with DON was 100% and 22.2% for the samples from Sumadija, Kolubara and City of Belgrade, districts, respectively. The highest number of AFB1 positive samples was found in Sumadija district (87.5%), while in the City of Belgrade and Podunavlje districts, 55.56% and 50% AFB1 positive samples were established, respectively. Generally, remarkable infection of all the tested samples with toxigenic fungal species from Aspergillus, Fusarium and Penicillium genera were recorded. In addition, high contamination with mycotoxins ZEA, DON and AFB1 were also recorded; nevertheless, only in one sample the level of DON exceeded the allowed legal limit (1750 ?g kg-1) according to Regulation for unprocessed maize. Therefore, permanent mycological and mycotoxicological analyses of maize grain are necessary for risk assessment of fungal and mycotoxin contamination throughout the food chain.

scholarly journals Comparison of Three Methods for Determining Aflatoxins in Melon Seeds

1996 ◽  
Vol 79 (6) ◽  
pp. 1330-1335 ◽  
Author(s):  
Vincent P Diprossimo ◽  
Emil G Malek
Keyword(s):  
Aflatoxin B1 ◽  
Aflatoxin Contamination ◽  
The Other ◽  
Contamination Level ◽  
Total Aflatoxin ◽  
The Third ◽  
Acetone Water ◽  
Melon Seeds ◽  
Layer Chromatography ◽  
Better Than

Abstract The suitability of 3 methods for determining aflatoxins in melon seeds was examined. The first 2 are the Contaminants Branch (CB) method and the Best Foods (BF) method, both official methods for determining aflatoxins in peanuts and peanut products. The third method, the modified CB method–Rapid Modification of the Cottonseed (CB-RCSMod) method, devised in this work, was derived by combining steps from the CB method and the Rapid Modification of the Cottonseed method. The CB method was superior to the other 2 methods for quantitation of aflatoxins. It gave better recoveries and cleaner extracts that exhibit less fluorescent interference for thin-layer chromatography (TLC) than the BF method. Also, its solvent efficiency was better than that of the CB-RCS-Mod method. With the CB method, recoveries from spiked samples were 85.0% for aflatoxin B1 and 90.0% for anatoxin B2. Recoveries of G1 aflatoxins were more variable, averaging 90.0% for aflatoxin d and 72.5% for aflatoxin G2. Total aflatoxin recovery was 86.5% for the CB method. At a low aflatoxin contamination level (8 μg B1/kg sample), aflatoxin B1 was detectable by the CB method but not by the BF method. Detection of aflatoxins in BF method sample extracts by TLC was not improved by the use of chloroform–acetone–water (88 + 12 + 1), benzene–ethanol–water, or ether–methanol–water (96 + 3 +1) in place of the standard chloroform–acetone (88 + 12) developer. Use of ether–methanol–water (96 + 3 + 1) for detecting aflatoxins by TLC in the CB method extracts increased interference compared with the standard chloroform–acetone (88 + 12) developer.

Storage practices and aflatoxin B1 contamination of ‘egusi’ melon seeds in Nigeria

2004 ◽  
Vol 44 (3) ◽  
pp. 150-153 ◽  
Author(s):  
SA Bankole ◽  
OA Lawal ◽  
A Adebanjo
Keyword(s):  
Aflatoxin B1 ◽  
Melon Seeds

scholarly journals Mycobiota and Mycotoxins Contaminating Rice Grains in El Minia, Governorate, Egypt

2019 ◽  
Vol 16 (1) ◽  
pp. 167-178
Author(s):  
A.M. Moharram ◽  
M.M. Yasser ◽  
M.A. Sayed ◽  
O.A. Omar ◽  
M.M.M. Idres
Keyword(s):  
Ochratoxin A ◽  
Aspergillus Flavus ◽  
Aflatoxin B1 ◽  
Penicillium Chrysogenum ◽  
Agar Medium ◽  
Natural Occurrence ◽  
Liquid Cultures ◽  
Rice Grains ◽  
Rice Samples ◽  
Prevalent Species

The mycological analysis of 51 samples of rice grains collected from different localities in El-Minia Governorate revealed the isolation of 54 species of fungi belonging to 21 genera. Most common mycobiota (genera) were Aspergillus and Penicillium being isolated from 96.07% and 54.9% of samples contributing 63.08% and 21.89% of total fungal counts. The prevalent species were represented by Aspergillus flavus, A. candidus, A. niger, Penicillium chrysogenum, P. islandicum especially on Dichloran Rose Bengal Chloramphenicol Agar medium (DRBC). These species in addition to some osmophilic fungi including A. chevalieri, A. montevidensis, A. rubrum were also common when Dichloran Glycerol agar (DG18) was used for the culturing of rice samples. About 12.5% of samples analysed for natural occurrence of mycotoxins were contaminated either with Aflatoxin – B1 (100-200 µg/ kg), ochratoxin –A (50-100 µg/ kg) or sterigmatocystin (10-20 µg/ kg). The majority of fungal strains tested for their mycotoxin production in liquid cultures were able to produce variable levels of aflatoxin B1, Aflatoxin G1 , Ochratoxin –A , terrein , gliotoxin and fumagillin

Natural occurrence of aflatoxin B1 in sorghum grown in different geographical regions of India

2012 ◽  
Vol 92 (12) ◽  
pp. 2416-2420 ◽  
Author(s):  
Chamarthy Venkata Ratnavathi ◽  
Vadabalija Venkata Komala ◽  
Bathula Swaroop Vijay Kumar ◽  
Indra Kanta Das ◽  
Jagannath Vishnu Patil
Keyword(s):  
Aflatoxin B1 ◽  
Natural Occurrence ◽  
Geographical Regions

scholarly journals Natural occurrence of aflatoxin B1 and aflatoxin M1 in “halva” and its ingredients

Food Control ◽  
2013 ◽  
Vol 34 (2) ◽  
pp. 404-407 ◽  
Author(s):  
Shahzad Zafar Iqbal ◽  
Muhammad Rafique Asi ◽  
S. Jinap
Keyword(s):  
Aflatoxin B1 ◽  
Aflatoxin M1 ◽  
Natural Occurrence

Occurrence of aflatoxin B1 in food products derivable from ‘egusi’ melon seeds consumed in southwestern Nigeria

Food Control ◽  
2010 ◽  
Vol 21 (7) ◽  
pp. 974-976 ◽  
Author(s):  
Samuel A. Bankole ◽  
Adedotun A. Adenusi ◽  
O.S. Lawal ◽  
O.O. Adesanya
Keyword(s):  
Aflatoxin B1 ◽  
Food Products ◽  
Southwestern Nigeria ◽  
Melon Seeds
Sign in / Sign up

Export Citation Format

Share Document