Storage practices and aflatoxin B1 contamination of ‘egusi’ melon seeds in Nigeria

2004 ◽  
Vol 44 (3) ◽  
pp. 150-153 ◽  
Author(s):  
SA Bankole ◽  
OA Lawal ◽  
A Adebanjo
Keyword(s):  
Aflatoxin B1 ◽  
Melon Seeds

Natural occurrence of moulds and aflatoxin B1 in melon seeds from markets in Nigeria

2004 ◽  
Vol 42 (8) ◽  
pp. 1309-1314 ◽  
Author(s):  
S.A Bankole ◽  
B.M Ogunsanwo ◽  
O.O Mabekoje
Keyword(s):  
Aflatoxin B1 ◽  
Natural Occurrence ◽  
Melon Seeds

Fungal contamination and aflatoxin B1 of ‘egusi’ melon seeds in Nigeria

Food Control ◽  
2006 ◽  
Vol 17 (10) ◽  
pp. 814-818 ◽  
Author(s):  
S.A. Bankole ◽  
B.M. Ogunsanwo ◽  
A. Osho ◽  
G.O. Adewuyi
Keyword(s):  
Aflatoxin B1 ◽  
Fungal Contamination ◽  
Melon Seeds

scholarly journals Comparison of Three Methods for Determining Aflatoxins in Melon Seeds

1996 ◽  
Vol 79 (6) ◽  
pp. 1330-1335 ◽  
Author(s):  
Vincent P Diprossimo ◽  
Emil G Malek
Keyword(s):  
Aflatoxin B1 ◽  
Aflatoxin Contamination ◽  
The Other ◽  
Contamination Level ◽  
Total Aflatoxin ◽  
The Third ◽  
Acetone Water ◽  
Melon Seeds ◽  
Layer Chromatography ◽  
Better Than

Abstract The suitability of 3 methods for determining aflatoxins in melon seeds was examined. The first 2 are the Contaminants Branch (CB) method and the Best Foods (BF) method, both official methods for determining aflatoxins in peanuts and peanut products. The third method, the modified CB method–Rapid Modification of the Cottonseed (CB-RCSMod) method, devised in this work, was derived by combining steps from the CB method and the Rapid Modification of the Cottonseed method. The CB method was superior to the other 2 methods for quantitation of aflatoxins. It gave better recoveries and cleaner extracts that exhibit less fluorescent interference for thin-layer chromatography (TLC) than the BF method. Also, its solvent efficiency was better than that of the CB-RCS-Mod method. With the CB method, recoveries from spiked samples were 85.0% for aflatoxin B1 and 90.0% for anatoxin B2. Recoveries of G1 aflatoxins were more variable, averaging 90.0% for aflatoxin d and 72.5% for aflatoxin G2. Total aflatoxin recovery was 86.5% for the CB method. At a low aflatoxin contamination level (8 μg B1/kg sample), aflatoxin B1 was detectable by the CB method but not by the BF method. Detection of aflatoxins in BF method sample extracts by TLC was not improved by the use of chloroform–acetone–water (88 + 12 + 1), benzene–ethanol–water, or ether–methanol–water (96 + 3 +1) in place of the standard chloroform–acetone (88 + 12) developer. Use of ether–methanol–water (96 + 3 + 1) for detecting aflatoxins by TLC in the CB method extracts increased interference compared with the standard chloroform–acetone (88 + 12) developer.

Occurrence of aflatoxin B1 in food products derivable from ‘egusi’ melon seeds consumed in southwestern Nigeria

Food Control ◽  
2010 ◽  
Vol 21 (7) ◽  
pp. 974-976 ◽  
Author(s):  
Samuel A. Bankole ◽  
Adedotun A. Adenusi ◽  
O.S. Lawal ◽  
O.O. Adesanya
Keyword(s):  
Aflatoxin B1 ◽  
Food Products ◽  
Southwestern Nigeria ◽  
Melon Seeds

HISTOLOGICAL CHANGES IN THE INTESTINES OF POULTRY DURING FODDER INTOXICATION

2020 ◽  
Author(s):  
E.P. Dolgov ◽  
◽  
A.A. Abramov ◽  
E.V. Kuzminova ◽  
E.V. Rogaleva ◽  
...  
Keyword(s):  
Body Weight ◽  
Histological Examination ◽  
Aflatoxin B1 ◽  
Structural Changes ◽  
Clinical Manifestations ◽  
Feed Additives ◽  
The Body ◽  
Histological Changes ◽  
Beet Pulp

The article presents the data on the study of the influence of mycotoxins combination (T-2 toxin at the concentration of 0.095 mg/kg and aflatoxin B1 in the concentration of 0.019 mg/kg) on the body of quails and the results of pharmacocorrection of toxicosis with a complex consisting of beet pulp and lecithin. Structural changes in the intestines of quais at fodder mycotoxicosis are described. The use of antitoxic feed additives in poultry led to a weakening of the action of xenobiotics, which was confirmed by an increase in the safety of poultry and increase in body weight of quails, a decrease in the clinical manifestations of intoxication, as well as in positive changes in the structure of the intestine of the poultry during histological examination.

Reduction of Milk Contamination with Aflatoxin-M1 through Vaccination of Dairy Cattle with Aflatoxin-B1 Vaccine

2020 ◽  
Keyword(s):  
Dairy Cattle ◽  
Aflatoxin B1 ◽  
Booster Vaccination ◽  
Primary Vaccination ◽  
Nano Particles ◽  
Aflatoxin M1 ◽  
Serum Samples ◽  
Serum Albumins ◽  
Milk Contamination ◽  
Before And After

Aflatoxin M1 is one of mycotoxin derivatives, which is secreted in milk of dairy cattle fed on feed contaminated with Aflatoxin-B1 (AFB1). The current study was designed to prepare a vaccine against AFB1and to evaluate its efficacy in reducing or preventing secretion of AFM1 in milk. Aflatoxin-B1 was prepared, purified and transformed into oxime, then it was fixed on bovine serum albumins. The AFB1-BSA conjugate was adjuvanted with Gold Nano particles then Montanide ISA 206. The prepared vaccine was used for immunization of rabbits by S/c routes as 100 µg/dose and dairy cattle by I/M routes as 500 µg/dose. The vaccinated animals were boosted at 3 weeks post primary immunization. Serum samples were collected and examined for the anti-AFB1 using AGPT. A mean titer of 15.2 AGPU/ml was detected at 2 weeks post primary vaccination then significantly increased till reached to 76.8 AGPU/ml at 6 weeks post Booster vaccination. All vaccinated rabbits were challenged with dose of 0.3 mg AFB1 toxin/Kg. The vaccinated rabbit showed 100% protection and no AFB1 toxin residue was detected in their livers. Milk samples were collected from non-vaccinated and AFB1-immunized dairy cattle then examined with ELISA for quantitation of AFM1 residues before and after vaccination. The results showed that the prepared AFB1 vaccine was safe, potent and able to reduce AFM1 release in milk of vaccinated heifers by 70%. So the vaccination of lactating animals with the AFB1vaccine might represent a valid tool for the prevention of AFM1 contamination of milk and dairy products.

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