Pattern differentiation of glandular cancerous cells and normal cells with cellular automata and evolutionary learning

2008 ◽  
Vol 34 (1) ◽  
pp. 337-346 ◽  
Author(s):  
Jong-Chen Chen ◽  
Chun-Ming Yeh ◽  
Jeh-En Tzeng
Keyword(s):  
Cellular Automata ◽  
Evolutionary Learning ◽  
Normal Cells ◽  
Pattern Differentiation ◽  
Cancerous Cells

scholarly journals Is the effect of Decitabine limited to hypomethylation and DNMTs?

2019 ◽  
Author(s):  
Sina Dalvand ◽  
Amin Namdari ◽  
Ashraf Alemi ◽  
Mohammad Hassan Meshkibaf ◽  
Sam Setayesh ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mrna Expression ◽  
Histone Deacetylases ◽  
Dna Methyltransferase ◽  
Chromatin Condensation ◽  
Dna Demethylation ◽  
Histone Tails ◽  
Normal Cells ◽  
Before And After ◽  
Cancerous Cells

Abstract Background: Histone modifications play a crucial role in chromatin structure. Among enzymes, which regulate these processes, histone deacetylases (HDACs) can remove acetyl groups from histone tails, thus increasing their interaction with DNA and leading to chromatin condensation. 5-Aza-2′-deoxycytidine (AZad) or Decitabine is a potent hypomethylating agent that incorporates into DNA and traps DNA methyltransferase in the form of a covalent protein–DNA adduct. Azad, not only change the gene expression through demethylation of the gene's promoter, but it also can change gene expression independently from DNA demethylation. So, the present study was to distinguish whether AZad in addition to inhibitory effects on DNA methyltransferase, can change HDAC3 and HDAC7 mRNA expression in NALM-6, HL-60 cancer cell lines. Methods: HL-60, NALM-6 and normal cells were cultured, and the treatment dose of the AZad was obtained (1µM) by the MTT test. Finally, HDAC3 and HDAC7 mRNA expression were measured by Real Time PCR in HL-60 and NALM-6 cancerous cells before and after treatment. In addition, HDAC3 and HDAC7 mRNA expression in un-treated HL-60 and NALM-6 cancerous cells were compared to the normal cells. Results: Our result revealed that expression of HDAC3 and HDAC7, in HL-60 and NALM-6 cells increases as compared to normal cells. After treatment of HL-60 and NALM-6 cells with AZad, HDAC3 and HDAC7 mRNA expression were decreased significantly. Conclusions: Our data showed, the effects of AZad are not limited to direct hypomethylation of DNMTs but it can indirectly affect other epigenetic factors, such as HDACs activity, through converging pathways. Keywords: HDAC3 ; HDAC7 ; HL-60; NALM-6 ; Decitabine ; AZad

scholarly journals Vitamin C − protective role in oxidative stress conditions induced in human normal colon cells by label free Raman spectroscopy and imaging

2021 ◽  
Author(s):  
Karolina Beton ◽  
Beata Brozek-Pluska
Keyword(s):  
Oxidative Stress ◽  
Raman Spectroscopy ◽  
Vitamin C ◽  
Human Colon ◽  
Protective Role ◽  
Spectroscopic Methods ◽  
Label Free ◽  
Normal Cells ◽  
Colon Cells ◽  
Cancerous Cells

Colorectal cancer is the second most frequently diagnosed cancer worldwide. Conventional diagnostics methods of colorectal cancer, can detect it in advanced stage. Spectroscopic methods, including Raman spectroscopy and imaging, are becoming more and more popular in medical applications, and allow fast, precise and unambiguous differentiation of healthy and cancerous samples. the most important advantage of Raman spectroscopy is ability to identify biomarkers that help in differentiation of healthy and cancerous cells based on biochemistry of sample and spectra typical for: lipids, proteins, DNA. The aim of the study was to evaluate the biochemical and structural features of human colon cell lines based on Raman spectroscopy and imaging: normal cells CCD-18 Co, normal cells CCD-18 Co under oxidative stress conditions, normal cells CCD-18 Co at first treated by using tert-Butyl hydroperoxide and then supplemented by vitamin C in high concentration to show the protective role of vitamin C in micromolar concentrations against ROS by spectroscopic methods. Raman data obtained for normal cells injured by ROS were compared with spectra typical for cancerous cells. Statistically assisted analysis has shown that normal, ROS injured and cancerous human colon cells can be distinguished based on their unique vibrational properties. The research carried out proves that label-free Raman spectroscopy may play an important role in clinical diagnostics differentiation of normal and cancerous colon cells and may be a source of intraoperative information supporting histopathological analysis.

A new copper complex enhanced apoptosis in human breast cancerous cells without considerable effects on normal cells

Gene Reports ◽  
2019 ◽  
Vol 17 ◽  
pp. 100475
Author(s):  
Fatemeh Mohammadizadeh ◽  
Mehdi Mahmoodi ◽  
Azadeh Rezaei ◽  
Maryam Mohamadi ◽  
Mohammad Reza Hajizadeh ◽  
...  
Keyword(s):  
Copper Complex ◽  
Human Breast ◽  
Normal Cells ◽  
Cancerous Cells

Characterization of endogenous fluorescence in nonsmall lung cancerous cells: A comparison with nonmalignant lung normal cells

2020 ◽  
Vol 13 (5) ◽  
Author(s):  
Kamlesh Awasthi ◽  
Feng‐Lin Chang ◽  
Pei‐Ying Hsieh ◽  
Hsin‐Yun Hsu ◽  
Nobuhiro Ohta
Keyword(s):  
Normal Cells ◽  
Endogenous Fluorescence ◽  
Cancerous Cells

Quantitative Cytotoxicity, Cellular Uptake and Radioprotection Effect of Cerium Oxide Nanoparticles in MRC-5 Normal Cells and MCF-7 Cancerous Cells

2018 ◽  
Vol 8 (3) ◽  
pp. 769-777 ◽  
Author(s):  
Nouraddin Abdi Goushbolagh ◽  
Bagher Farhood ◽  
Akram Astani ◽  
Abolfazl Nikfarjam ◽  
Mojgan Kalantari ◽  
...  
Keyword(s):  
Cerium Oxide ◽  
Cellular Uptake ◽  
Cerium Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Normal Cells ◽  
Cancerous Cells ◽  
Mcf 7

scholarly journals Detection of Tumor Cells in Brain using Cellular Automata with Image Segmentation and Edge Detection.

2019 ◽  
Vol 9 (4) ◽  
pp. 11-13
Keyword(s):  
Image Segmentation ◽  
Brain Tumor ◽  
Cellular Automata ◽  
Edge Detection ◽  
Human Life ◽  
Computation Time ◽  
Mri Scan ◽  
Magnetic Resonance Imaging Mri ◽  
Medical Examinations ◽  
Cancerous Cells

Tumor growth or, growth of cancerous cells is a big challenge in today’s medical word. When dealing with human life, the detection of tumors through computers has to be highly accurate. Thus we require the assistance of computer in medical examinations, so that we will get very low rate of false cases. Brain tumor, in today’s world, is seen as most threatening and life taking disease. In order to detect brain tumor more accurately in lesser time, many techniques have already been proposed using image segmentation and edge detection. In our paper we propose a technique which is more efficient to detect brain tumor where edge detection through cellular automata have been used from Magnetic Resonance Imaging (MRI) scan images. It processes these images, and determines the area affected by using segmentation and edge detection with cellular automata. Simulated work is completed with the help of Simulink in MATLAB. Regarding this particular topic there are many studies, however our proposal of combination of both segmentation and edge detection through cellular automata shows better results as compared to combining segmentation with classical edge detection in term of computation time and clarity. This will help in efficiency of detecting brain tumor and later in its removal.

Application of carbon dots in nucleolus imaging to distinguish cancerous cells from normal cells

2020 ◽  
pp. 129156
Author(s):  
Boye Zhang ◽  
Qianqian Duan ◽  
Haichao Zhao ◽  
Yixia Zhang ◽  
Xiaoning Li ◽  
...  
Keyword(s):  
Carbon Dots ◽  
Normal Cells ◽  
Cancerous Cells

Synthesis of pH-responsive cyclometalated iridium(III) complex and its application in selective killing of cancerous cells

2021 ◽  
Author(s):  
Peng Yang ◽  
Shaoqing Zhang ◽  
Ke Wang ◽  
Honglan Qi
Keyword(s):  
Photodynamic Therapy ◽  
Ph Responsive ◽  
Normal Cells ◽  
Cancerous Cells

Cyclometalated iridium(III) complexes are promising candidates as photosensitizers (PSs) in photodynamic therapy (PDT). The challenge in PDT is selective killing of cancerous cells against its neighboring normal cells. In this...

Mitochondria-targetable carbon quantum dots for differentiating cancerous cells from normal cells

Nanoscale ◽  
2017 ◽  
Vol 9 (46) ◽  
pp. 18368-18378 ◽  
Author(s):  
Ge Gao ◽  
Yao-Wen Jiang ◽  
Jingjing Yang ◽  
Fu-Gen Wu
Keyword(s):  
Quantum Dots ◽  
Cell Differentiation ◽  
Solvothermal Synthesis ◽  
Carbon Quantum Dots ◽  
Normal Cells ◽  
Cancerous Cell ◽  
One Step ◽  
Cancerous Cells

One-step solvothermal synthesis of highly fluorescent and multifunctional carbon quantum dots for mitochondrial tracking and normal/cancerous cell differentiation.

Selective cytotoxic activity of isolated compounds from Globimetula dinklagei and Phragmanthera capitata (Loranthaceae)

2020 ◽  
Vol 75 (5-6) ◽  
pp. 135-144
Author(s):  
Emmanuel Mfotie Njoya ◽  
Hermine L.D. Maza ◽  
Pierre Mkounga ◽  
Ulrich Koert ◽  
Augustin E. Nkengfack ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Caspase 3 ◽  
Selectivity Index ◽  
Hydroxyl Group ◽  
Lung Cells ◽  
Normal Cells ◽  
Selective Cytotoxicity ◽  
Structure Activity ◽  
Cancerous Cells ◽  
Mcf 7

AbstractThis study aimed to evaluate the selective cytotoxicity of six natural compounds on four cancerous cells (MCF-7, HeLa, Caco-2 and A549) and two normal intestinal and lung cells (Hs1.Int and Wl-38) cells. We also attempted to analyze basically the structure–activity relationships and to understand the mechanism of action of active compounds using the Caspase-Glo® 3/7 kit. Globimetulin B (2) isolated from Globimetula dinklagei was significantly cytotoxic on cancerous cells with 50% inhibitory concentrations (IC50) ranging from 12.75 to 37.65 μM and the selectivity index (SI) values varying between 1.13 and 3.48 against both normal cells. The compound 3-O-β-d-glucopyranosyl-28-hydroxy-α-amyrin (5) isolated from Phragmanthera capitata exhibited the highest cytotoxic activity on HeLa cells with the IC50 of 6.88 μM and the SI of 5.20 and 8.71 against Hs1.Int and Wl-38 cells, respectively. A hydroxyl group at C-3 of compounds was suggested as playing an important role in the cytotoxic activity. The induction of caspase-3 and -7 activity represents some proof that apoptosis has occurred in treated cells. Globimetulin B (2) selectively killed cancer cells with less toxicity to non-cancerous cells as compared to conventional doxorubicin therapy.

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