A new copper complex enhanced apoptosis in human breast cancerous cells without considerable effects on normal cells

Gene Reports ◽  
2019 ◽  
Vol 17 ◽  
pp. 100475
Author(s):  
Fatemeh Mohammadizadeh ◽  
Mehdi Mahmoodi ◽  
Azadeh Rezaei ◽  
Maryam Mohamadi ◽  
Mohammad Reza Hajizadeh ◽  
...  
Keyword(s):  
Copper Complex ◽  
Human Breast ◽  
Normal Cells ◽  
Cancerous Cells

scholarly journals Is the effect of Decitabine limited to hypomethylation and DNMTs?

2019 ◽  
Author(s):  
Sina Dalvand ◽  
Amin Namdari ◽  
Ashraf Alemi ◽  
Mohammad Hassan Meshkibaf ◽  
Sam Setayesh ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mrna Expression ◽  
Histone Deacetylases ◽  
Dna Methyltransferase ◽  
Chromatin Condensation ◽  
Dna Demethylation ◽  
Histone Tails ◽  
Normal Cells ◽  
Before And After ◽  
Cancerous Cells

Abstract Background: Histone modifications play a crucial role in chromatin structure. Among enzymes, which regulate these processes, histone deacetylases (HDACs) can remove acetyl groups from histone tails, thus increasing their interaction with DNA and leading to chromatin condensation. 5-Aza-2′-deoxycytidine (AZad) or Decitabine is a potent hypomethylating agent that incorporates into DNA and traps DNA methyltransferase in the form of a covalent protein–DNA adduct. Azad, not only change the gene expression through demethylation of the gene's promoter, but it also can change gene expression independently from DNA demethylation. So, the present study was to distinguish whether AZad in addition to inhibitory effects on DNA methyltransferase, can change HDAC3 and HDAC7 mRNA expression in NALM-6, HL-60 cancer cell lines. Methods: HL-60, NALM-6 and normal cells were cultured, and the treatment dose of the AZad was obtained (1µM) by the MTT test. Finally, HDAC3 and HDAC7 mRNA expression were measured by Real Time PCR in HL-60 and NALM-6 cancerous cells before and after treatment. In addition, HDAC3 and HDAC7 mRNA expression in un-treated HL-60 and NALM-6 cancerous cells were compared to the normal cells. Results: Our result revealed that expression of HDAC3 and HDAC7, in HL-60 and NALM-6 cells increases as compared to normal cells. After treatment of HL-60 and NALM-6 cells with AZad, HDAC3 and HDAC7 mRNA expression were decreased significantly. Conclusions: Our data showed, the effects of AZad are not limited to direct hypomethylation of DNMTs but it can indirectly affect other epigenetic factors, such as HDACs activity, through converging pathways. Keywords: HDAC3 ; HDAC7 ; HL-60; NALM-6 ; Decitabine ; AZad

scholarly journals Vitamin C − protective role in oxidative stress conditions induced in human normal colon cells by label free Raman spectroscopy and imaging

2021 ◽  
Author(s):  
Karolina Beton ◽  
Beata Brozek-Pluska
Keyword(s):  
Oxidative Stress ◽  
Raman Spectroscopy ◽  
Vitamin C ◽  
Human Colon ◽  
Protective Role ◽  
Spectroscopic Methods ◽  
Label Free ◽  
Normal Cells ◽  
Colon Cells ◽  
Cancerous Cells

Colorectal cancer is the second most frequently diagnosed cancer worldwide. Conventional diagnostics methods of colorectal cancer, can detect it in advanced stage. Spectroscopic methods, including Raman spectroscopy and imaging, are becoming more and more popular in medical applications, and allow fast, precise and unambiguous differentiation of healthy and cancerous samples. the most important advantage of Raman spectroscopy is ability to identify biomarkers that help in differentiation of healthy and cancerous cells based on biochemistry of sample and spectra typical for: lipids, proteins, DNA. The aim of the study was to evaluate the biochemical and structural features of human colon cell lines based on Raman spectroscopy and imaging: normal cells CCD-18 Co, normal cells CCD-18 Co under oxidative stress conditions, normal cells CCD-18 Co at first treated by using tert-Butyl hydroperoxide and then supplemented by vitamin C in high concentration to show the protective role of vitamin C in micromolar concentrations against ROS by spectroscopic methods. Raman data obtained for normal cells injured by ROS were compared with spectra typical for cancerous cells. Statistically assisted analysis has shown that normal, ROS injured and cancerous human colon cells can be distinguished based on their unique vibrational properties. The research carried out proves that label-free Raman spectroscopy may play an important role in clinical diagnostics differentiation of normal and cancerous colon cells and may be a source of intraoperative information supporting histopathological analysis.

scholarly journals β-Carboline copper complex as a potential mitochondrial-targeted anticancer chemotherapeutic agent: Favorable attenuation of human breast cancer MCF7 cells via apoptosis

2020 ◽  
Vol 27 (8) ◽  
pp. 2164-2173
Author(s):  
Rais Ahmad Khan ◽  
Mohammad Rashid Khan ◽  
Mohammad Usman ◽  
Fatima Sayeed ◽  
Huda A. Alghamdi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Copper Complex ◽  
Human Breast Cancer ◽  
Chemotherapeutic Agent ◽  
Human Breast ◽  
Mcf7 Cells

Characterization of endogenous fluorescence in nonsmall lung cancerous cells: A comparison with nonmalignant lung normal cells

2020 ◽  
Vol 13 (5) ◽  
Author(s):  
Kamlesh Awasthi ◽  
Feng‐Lin Chang ◽  
Pei‐Ying Hsieh ◽  
Hsin‐Yun Hsu ◽  
Nobuhiro Ohta
Keyword(s):  
Normal Cells ◽  
Endogenous Fluorescence ◽  
Cancerous Cells

Quantitative Cytotoxicity, Cellular Uptake and Radioprotection Effect of Cerium Oxide Nanoparticles in MRC-5 Normal Cells and MCF-7 Cancerous Cells

2018 ◽  
Vol 8 (3) ◽  
pp. 769-777 ◽  
Author(s):  
Nouraddin Abdi Goushbolagh ◽  
Bagher Farhood ◽  
Akram Astani ◽  
Abolfazl Nikfarjam ◽  
Mojgan Kalantari ◽  
...  
Keyword(s):  
Cerium Oxide ◽  
Cellular Uptake ◽  
Cerium Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Normal Cells ◽  
Cancerous Cells ◽  
Mcf 7

Synthesis of d-Mannose Capped Silicon Nanoparticles and Their Interactions with MCF-7 Human Breast Cancerous Cells

2013 ◽  
Vol 5 (15) ◽  
pp. 7384-7391 ◽  
Author(s):  
Jayshree H. Ahire ◽  
Isabelle Chambrier ◽  
Anja Mueller ◽  
Yongping Bao ◽  
Yimin Chao
Keyword(s):  
Silicon Nanoparticles ◽  
Human Breast ◽  
Cancerous Cells ◽  
Mcf 7

scholarly journals Targeting Estrogen Receptor Sites in Human Breast Cancer Cell Line T47D With Copper Conjugates of Nonsteriodal Anti-inflammatory Drug Derivatives: Antiproliferative Activity of Ketoprofen Derivative and its Copper Complex

2001 ◽  
Vol 8 (2) ◽  
pp. 73-77 ◽  
Author(s):  
Dilip Kumar Saha ◽  
Shreelekha Padhye ◽  
Subhash Padhye
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Copper Complex ◽  
Breast Cancer Cell Line ◽  
Human Breast Cancer ◽  
Cancer Cell Line ◽  
Human Breast Cancer Cell ◽  
Human Breast

A square planar copper complex of derivatized NSAID drug (Ketoprofen thiosemicarbazone [3-benzoyl-α -methyl benzene acetic acid thiosemicarbazone]), is characterized by elemental analysis, spectroscopy, electrochemistry and magnetic susceptibility studies which exhibits dose-dependent and enhanced antiproliferative effects on human breast cancer cell line T47D rich in progesterone receptors.

scholarly journals Comparison on the Cancer Specific Cytotoxicity of Three Gingers (Zingiber officinale Rosc) Leaves Varieties from Indonesia

2017 ◽  
Vol 9 (1) ◽  
Author(s):  
Susanti S. ◽  
Kumoro C. A. ◽  
Santoso I. S. ◽  
Murwanti R. ◽  
Suzery M. ◽  
...  
Keyword(s):  
Cell Viability ◽  
Cancer Cell ◽  
Detrimental Effect ◽  
Zingiber Officinale ◽  
Assay Method ◽  
Human Breast ◽  
Normal Cells ◽  
Specific Cytotoxicity ◽  
Reduce Cell Viability ◽  
Mcf 7

This study was performed to get more insight the cancer specific cytotoxicity of ginger leaf (GL). Three GL varieties (Gajah, Emprit, and Red) were extracted and fractionated. Each etyl acetate fraction in concentration of 200 µg/ml was tested about specific cytotoxicity toward cancer dan normal cells. Cancer cell lines used in this study were human colorectal (HCT116) and human breast (MCF-7 and T47D) cancer while normal cell line was human fibroblast (KMST-6). Based MTS assay method, the results showed Gajah and Emprit GL more significantly reduce cell viability of HCT116 and T47D than Red GL although there was no difference on the efficacy of both varieties. All varieties of GL also significantly reduce cell viability of MCF-7 compare to PBS control. However, there were not significant differences between those GL varieties on their effectiveness against MCF-7. In contrast, there were no effects on the KMST-6 due to all GL varieties treatment compare with PBS control. All data suggested that GL treatment only inhibited in the cancer cells without detrimental effect in the normal cells. Effectiveness of GL against cancer cell varies depend on the varieties. Gajah and Emprit GL are better varieties possess the cancer specific cytotoxicity that merits to be developed as promising chemo preventive agent in the future.

Sign in / Sign up

Export Citation Format

Share Document