Effect of pH on the interactions of doxorubicin with charged lipid monolayers containing 1,2-dimyristoyl-sn-glycero-3-phospho-l-serine - An important component of cancer cell membranes

2018 ◽  
Vol 280 ◽  
pp. 229-237 ◽  
Author(s):  
Dorota Matyszewska ◽  
Sandra Moczulska
Keyword(s):  
Cancer Cell ◽  
Cell Membranes ◽  
Lipid Monolayers ◽  
Effect Of Ph

664: CXCL12/CXCR4 Trans-Activation of HER2 in Lipid Rafts of Prostate Cancer Cell Membranes Promotes Bone Metastasis

2007 ◽  
Vol 177 (4S) ◽  
pp. 223-223
Author(s):  
Sreenivasa R. Chinni ◽  
Hamilto Yamamoto ◽  
Zhong Dong ◽  
Aaron Sabbota ◽  
Sanaa Nabha ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Bone Metastasis ◽  
Lipid Rafts ◽  
Cancer Cell ◽  
Cell Membranes ◽  
Prostate Cancer Cell

Penetration and preferential binding of charged nanoparticles to mixed lipid monolayers: interplay of lipid packing and charge density

Soft Matter ◽  
2020 ◽  
Author(s):  
Anurag Chaudhury ◽  
Koushik Debnath ◽  
Wei Bu ◽  
Nikhil R. Jana ◽  
Jaydeep Kumar Basu
Keyword(s):  
Charge Density ◽  
Biomedical Applications ◽  
Cell Membranes ◽  
Lipid Monolayers ◽  
Cytotoxic Effects ◽  
Lipid Packing ◽  
Preferential Binding ◽  
Charged Nanoparticles

Designing of nanoparticles (NPs) for biomedical applications or mitigating their cytotoxic effects require microscopic understanding of their interactions with cell membranes. Such insight is best obtained by studying model biomembranes...

scholarly journals The n-10 Fatty Acids Family in the Lipidome of Human Prostatic Adenocarcinoma Cell Membranes and Extracellular Vesicles

2020 ◽  
Author(s):  
Carla Ferreri ◽  
Anna Sansone ◽  
Sandra Buratta ◽  
Lorena Urbanelli ◽  
Eva Costanzi ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Cell Lines ◽  
Cancer Cell ◽  
Extracellular Vesicles ◽  
Cell Membranes ◽  
Saturated Fatty Acids ◽  
Prostatic Adenocarcinoma ◽  
Fatty Acid Isomer ◽  
Adenocarcinoma Cell

A new pathway leading to the n-10 fatty acid series has been recently evidenced, starting from sapienic acid - a monounsaturated fatty acid (MUFA) resulting from the transformation of palmitic acid by delta-6 desaturase. Sapienic acid attracts attention as novel marker of cancer cell plasticity. Here, we analyzed fatty acids including the n-10 fatty acid contents, and compared for the first time cell membranes and the corresponding extracellular vesicles (EV) of two human prostatic adenocarcinoma cell lines of different aggressiveness (PC3 and LNCaP). The n-10 components were 9-13% of the total fatty acids in both cancer cell lines and EVs, with total MUFA levels significantly higher in EVs of the most aggressive cell type (PC3). High sapienic/palmitoleic ratios indicated the preference for delta-6 vs. delta-9 desaturase enzymatic activity in these cell lines. The expressions analysis of enzymes involved in desaturation and elongation by qRT-PCR showed a higher desaturase activity in PC3 and a higher elongase activity toward polyunsaturated fatty acids than toward saturated fatty acids, compared to LNCaP cells. Our results improve the present knowledge in cancer fatty acid metabolism and lipid phenotypes, highlighting EV lipidomics to monitor positional fatty acid isomer profiles and MUFA levels in cancer.

The impact of phosphatidylserine exposure on cancer cell membranes on the activity of the anticancer peptide HB43

FEBS Journal ◽  
2021 ◽  
Author(s):  
Claudia Herrera‐León ◽  
Francisco Ramos‐Martín ◽  
Viviane Antonietti ◽  
Pascal Sonnet ◽  
Nicola D’Amelio
Keyword(s):  
Cancer Cell ◽  
Cell Membranes ◽  
Anticancer Peptide ◽  
Phosphatidylserine Exposure ◽  
The Impact

Super-resolution imaging of cancer-associated carbohydrates using aptamer probes

Nanoscale ◽  
2019 ◽  
Vol 11 (31) ◽  
pp. 14879-14886 ◽  
Author(s):  
Yingying Jing ◽  
Junling Chen ◽  
Lulu Zhou ◽  
Jiayin Sun ◽  
Mingjun Cai ◽  
...  
Keyword(s):  
Cancer Cell ◽  
Cell Membranes ◽  
Super Resolution ◽  
High Expression ◽  
Resolution Imaging ◽  
Potential Interactions

Relying on fluorophore-conjugated aptamers, we revealed the high expression and clustered distribution of globo H on cancer cell membranes and uncovered the potential interactions between cancer-associated carbohydrates via super-resolution imaging.

Erythrocyte membrane nano-capsules: biomimetic delivery and controlled release of photothermal–photochemical coupling agents for cancer cell therapy

2020 ◽  
Vol 49 (8) ◽  
pp. 2645-2651 ◽  
Author(s):  
Shihao Li ◽  
Lin Zhang
Keyword(s):  
Controlled Release ◽  
Cell Therapy ◽  
Blood Cell ◽  
Erythrocyte Membrane ◽  
Cancer Cell ◽  
Red Blood Cell ◽  
Photothermal Therapy ◽  
Cell Membranes ◽  
Coupling Agents ◽  
Red Blood Cell Membranes

A biomimetic nanoreactor Au/TiO2@RBC was fabricated for photothermal therapy by co-encapsulating photothermal agents and photocatalysts in red blood cell membranes.

scholarly journals Harnessing platelets as functional vectors for contrast enhanced ultrasound imaging and fluorescence imaging

RSC Advances ◽  
2019 ◽  
Vol 9 (72) ◽  
pp. 41993-41999
Author(s):  
Chuang Gao ◽  
Pravin Bhattarai ◽  
Yiming Zhou ◽  
Nisi Zhang ◽  
Sadaf Hameed ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Fluorescence Imaging ◽  
Cancer Cell ◽  
Ultrasound Imaging ◽  
Blood Cells ◽  
Recent Progress ◽  
Cell Membranes ◽  
Contrast Enhanced Ultrasound ◽  
Contrast Enhanced

The recent progress in the development of highly biocompatible nanoplatforms mostly encompasses the use of biological excipients such as red blood cells, cancer cell membranes, and also platelets.

Characterization of β-Adrenergic Receptor Linked to Adenylate Cyclase in a Human Cancer Cell Line (COLO 16)

1978 ◽  
Vol 55 (1) ◽  
pp. 23-29
Author(s):  
T. J. Martin ◽  
S. R. Nahorski ◽  
N. H. Hunt ◽  
J. K. Dawborn ◽  
R. S. Loomes ◽  
...  
Keyword(s):  
Adenylate Cyclase ◽  
Cell Line ◽  
Cancer Cell ◽  
Adrenergic Receptor ◽  
Cell Membranes ◽  
Human Cancer ◽  
Specific Binding ◽  
Cancer Cell Line ◽  
Human Cancer Cell ◽  
Adrenergic Agonists

1. A human cancer cell line (COLO 16) derived originally from an epidermal squamous cell carcinoma was found to possess adenylate cyclase responsiveness to β-adrenergic agonists. 2. The adenylate cyclase response was characterized with respect to activation constants (KA) for various β-adrenergic agonists and inhibition constants (Ki) for antagonists. 3. Intact cells responded with dose-dependent increases in production of cyclic adenosine 3′:5′-monophosphate. 4. Properties of the β-adrenergic receptor were evaluated by using the specific binding of [3H]propranolol to cell membranes. Specific binding was saturable, with KD 5.79 nmol/l and binding sites 0.68 pmol/mg of protein. 5. Competition for binding to cell membranes was shown by β-adrenergic agonists and antagonists and was stereospecific. There was close agreement between the affinity of these various agents on adenylate cyclase and receptor binding. 6. It is likely that the β-adrenergic receptor-linked adenylate cyclase in COLO 16 cells represents persistence in a cancer cell line of a receptor present normally in epidermal cells.

scholarly journals Cell viability and electrical response of breast cancer cell treated in aqueous graphene oxide solution deposition on interdigitated electrode

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Muhammad M. Ramli ◽  
A. S. Rosman ◽  
N. S. Mazlan ◽  
M. F. Ahmad ◽  
D. S. C. Halin ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Graphene Oxide ◽  
Cell Viability ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Electrical Impedance ◽  
Cell Membranes ◽  
Electrical Response ◽  
Viable Cell ◽  
Normal Breast

AbstractBreast cancer is one of the most reported cancers that can lead to death. Despite the advances in diagnosis and treatment procedures, the possibility of cancer recurrences is still high in many cases. With that in consideration, researchers from all over the world are showing interest in the unique features of Graphene oxide (GO), such as its excellent and versatile physicochemical properties, to explore further its potential and benefits towards breast cancer cell treatment. In this study, the cell viability and electrical response of GO, in terms of resistivity and impedance towards the breast cancer cells (MCF7) and normal breast cells (MCF10a), were investigated by varying the pH and concentration of GO. Firstly, the numbers of MCF7 and MCF10a were measured after being treated with GO for 24 and 48 h. Next, the electrical responses of these cells were evaluated by using interdigitated gold electrodes (IDEs) that are connected to an LCR meter. Based on the results obtained, as the pH of GO increased from pH 5 to pH 7, the number of viable MCF7 cells decreased while the number of viable MCF10a slightly increased after the incubation period of 48 h. Similarly, the MCF7 also experienced higher cytotoxicity effects when treated with GO concentrations of more than 25 µg/mL. The findings from the electrical characterization of the cells observed that the number of viable cells has corresponded to the impedance of the cells. The electrical impedance of MCF7 decreased as the number of highly insulating viable cell membranes decreased. But in contrast, the electrical impedance of MCF10a increased as the number of highly insulating viable cell membranes increased. Hence, it can be deduced that the GO with higher pH and concentration influence the MCF7 cancer cell line and MCF10a normal breast cell.

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