Super-resolution imaging of cancer-associated carbohydrates using aptamer probes

Nanoscale ◽  
2019 ◽  
Vol 11 (31) ◽  
pp. 14879-14886 ◽  
Author(s):  
Yingying Jing ◽  
Junling Chen ◽  
Lulu Zhou ◽  
Jiayin Sun ◽  
Mingjun Cai ◽  
...  
Keyword(s):  
Cancer Cell ◽  
Cell Membranes ◽  
Super Resolution ◽  
High Expression ◽  
Resolution Imaging ◽  
Potential Interactions

Relying on fluorophore-conjugated aptamers, we revealed the high expression and clustered distribution of globo H on cancer cell membranes and uncovered the potential interactions between cancer-associated carbohydrates via super-resolution imaging.

scholarly journals Mechanistic Insights into Trop2 Clustering on Lung Cancer Cell Membranes Revealed by Super-resolution Imaging

ACS Omega ◽  
2020 ◽  
Vol 5 (50) ◽  
pp. 32456-32465
Author(s):  
Yilin Fu ◽  
Peiyan Hua ◽  
Yan Lou ◽  
Zihao Li ◽  
Meng Jia ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Cell ◽  
Cell Membranes ◽  
Super Resolution ◽  
Lung Cancer Cell ◽  
Resolution Imaging

scholarly journals Super-resolution imaging uncovers the nanoscopic segregation of polarity proteins in epithelia

2020 ◽  
Author(s):  
Pierre Mangeol ◽  
Dominique Massey-Harroche ◽  
Fabrice Richard ◽  
Pierre-François Lenne ◽  
André Le Bivic
Keyword(s):  
Cell Polarity ◽  
Super Resolution ◽  
Actin Organization ◽  
Resolution Imaging ◽  
Polarity Proteins ◽  
Endogenous Proteins ◽  
Apical Junctions ◽  
And Function ◽  
Potential Interactions ◽  
Nanoscale Level

AbstractEpithelial tissues acquire their integrity and function through the apico-basal polarization of their constituent cells. Proteins of the PAR and Crumbs complexes are pivotal to epithelial polarization, but the mechanistic understanding of polarization is challenging to reach, largely because numerous potential interactions between these proteins and others have been found, without clear hierarchy in importance. We identify the regionalized and segregated organization of members of the PAR and Crumbs complexes at epithelial apical junctions by imaging endogenous proteins using STED microscopy on Caco-2 cells, human and murine intestinal samples. Proteins organize in submicrometric clusters, with PAR3 overlapping with the tight junction (TJ) while PALS1-PATJ and aPKC-PAR6β form segregated clusters that are apical of the TJ and present in an alternated pattern related to actin organization. CRB3A is also apical of the TJ and weakly overlaps with other polarity proteins. This organization at the nanoscale level significantly simplifies our view on how polarity proteins could cooperate to drive and maintain cell polarity.

scholarly journals Super-resolution imaging of aquaporin-4 orthogonal arrays of particles in cell membranes

2012 ◽  
Vol 125 (18) ◽  
pp. 4405-4412 ◽  
Author(s):  
A. Rossi ◽  
T. J. Moritz ◽  
J. Ratelade ◽  
A. S. Verkman
Keyword(s):  
Cell Membranes ◽  
Super Resolution ◽  
Orthogonal Arrays ◽  
Aquaporin 4 ◽  
Orthogonal Arrays Of Particles ◽  
Resolution Imaging

scholarly journals Mechanistic insights into GLUT1 activation and clustering revealed by super-resolution imaging

2018 ◽  
Vol 115 (27) ◽  
pp. 7033-7038 ◽  
Author(s):  
Qiuyan Yan ◽  
Yanting Lu ◽  
Lulu Zhou ◽  
Junling Chen ◽  
Haijiao Xu ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Cell Membranes ◽  
Molecular Mechanisms ◽  
Glucose Transporter ◽  
Super Resolution ◽  
Average Diameter ◽  
Activation Mechanism ◽  
Membrane Expression ◽  
Close Relationship ◽  
Resolution Imaging

The glucose transporter GLUT1, a plasma membrane protein that mediates glucose homeostasis in mammalian cells, is responsible for constitutive uptake of glucose into many tissues and organs. Many studies have focused on its vital physiological functions and close relationship with diseases. However, the molecular mechanisms of its activation and transport are not clear, and its detailed distribution pattern on cell membranes also remains unknown. To address these, we first investigated the distribution and assembly of GLUT1 at a nanometer resolution by super-resolution imaging. On HeLa cell membranes, the transporter formed clusters with an average diameter of ∼250 nm, the majority of which were regulated by lipid rafts, as well as being restricted in size by both the cytoskeleton and glycosylation. More importantly, we found that the activation of GLUT1 by azide or MβCD did not increase its membrane expression but induced the decrease of the large clusters. The results suggested that sporadic distribution of GLUT1 may facilitate the transport of glucose, implying a potential association between the distribution and activation. Collectively, our work characterized the clustering distribution of GLUT1 and linked its spatial structural organization to the functions, which would provide insights into the activation mechanism of the transporter.

scholarly journals Super-resolution Imaging Of Hemagglutinin Clusters In Cell Membranes

2009 ◽  
Vol 96 (3) ◽  
pp. 450a
Author(s):  
Travis Gould ◽  
Manasa V. Gudheti ◽  
Mudalige S. Gunewardene ◽  
Samuel T. Hess
Keyword(s):  
Cell Membranes ◽  
Super Resolution ◽  
Resolution Imaging

664: CXCL12/CXCR4 Trans-Activation of HER2 in Lipid Rafts of Prostate Cancer Cell Membranes Promotes Bone Metastasis

2007 ◽  
Vol 177 (4S) ◽  
pp. 223-223
Author(s):  
Sreenivasa R. Chinni ◽  
Hamilto Yamamoto ◽  
Zhong Dong ◽  
Aaron Sabbota ◽  
Sanaa Nabha ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Bone Metastasis ◽  
Lipid Rafts ◽  
Cancer Cell ◽  
Cell Membranes ◽  
Prostate Cancer Cell
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