Electrochemical DNA sensor based on three-dimensional folding paper device for specific and sensitive point-of-care testing

2012 ◽  
Vol 80 ◽  
pp. 334-341 ◽  
Author(s):  
Juanjuan Lu ◽  
Shenguang Ge ◽  
Lei Ge ◽  
Mei Yan ◽  
Jinghua Yu
Keyword(s):  
Point Of Care ◽  
Three Dimensional ◽  
Dna Sensor ◽  
Point Of Care Testing ◽  
Electrochemical Dna Sensor ◽  
Folding Paper ◽  
Paper Device

ELECTROCHEMICAL DNA SENSOR TECHNOLOGY FOR MONITORING OF DRUG–DNA INTERACTIONS

NANO ◽  
2008 ◽  
Vol 03 (04) ◽  
pp. 229-232 ◽  
Author(s):  
A. ERDEM ◽  
H. KARADENIZ ◽  
A. CALISKAN ◽  
A. VASEASHTA
Keyword(s):  
Drug Delivery ◽  
Small Molecules ◽  
Anticancer Drugs ◽  
Point Of Care ◽  
Sensor Technology ◽  
Electrochemical Biosensors ◽  
Dna Sensor ◽  
Dna Interactions ◽  
Electrochemical Dna Sensor

The objective of this investigation is to understand the nature and dynamics of binding small molecules to bio-macromolecules using electrochemical methods. The investigation pertaining to the design of site- and conformation-specific reagents provides a rationale for new studies of drug delivery design. Some anticancer drugs and DNA interactions have been undertaken by using a variety of techniques. Determination of interaction between DNA and DNA-targeted molecules would be valuable in the design of molecule-specific electrochemical biosensors for applications in diagnostics, development of drugs for chemotherapy, and as a biotechnological tool for DNA-based point-of-care diagnosis.

scholarly journals Three-Dimensional Paper-Based Microfluidic Analysis Device for Simultaneous Detection of Multiple Biomarkers with a Smartphone

Biosensors ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 187
Author(s):  
Seung Ho Baek ◽  
Chanyong Park ◽  
Jaehyung Jeon ◽  
Sungsu Park
Keyword(s):  
Point Of Care ◽  
Simultaneous Detection ◽  
Three Dimensional ◽  
Cost Effective ◽  
Smartphone Application ◽  
Accurate Diagnosis ◽  
Point Of Care Testing ◽  
Digital Light Processing ◽  
Multiple Biomarkers ◽  
Microfluidic Analysis

Paper-based microfluidic analysis devices (μPADs) have attracted attention as a cost-effective platform for point-of-care testing (POCT), food safety, and environmental monitoring. Recently, three-dimensional (3D)-μPADs have been developed to improve the performance of μPADs. For accurate diagnosis of diseases, however, 3D-μPADs need to be developed to simultaneously detect multiple biomarkers. Here, we report a 3D-μPADs platform for the detection of multiple biomarkers that can be analyzed and diagnosed with a smartphone. The 3D-μPADs were fabricated using a 3D digital light processing printer and consisted of a sample reservoir (300 µL) connected to 24 detection zones (of 4 mm in diameter) through eight microchannels (of 2 mm in width). With the smartphone application, eight different biomarkers related to various diseases were detectable in concentrations ranging from normal to abnormal conditions: glucose (0–20 mmol/L), cholesterol (0–10 mmol/L), albumin (0–7 g/dL), alkaline phosphatase (0–800 U/L), creatinine (0–500 µmol/L), aspartate aminotransferase (0–800 U/L), alanine aminotransferase (0–1000 U/L), and urea nitrogen (0–7.2 mmol/L). These results suggest that 3D-µPADs can be used as a POCT platform for simultaneous detection of multiple biomarkers.

scholarly journals Enhanced Sensing Behavior of Three-Dimensional Microfluidic Paper-Based Analytical Devices (3D-μPADs) with Evaporation-Free Enclosed Channels for Point-of-Care Testing

Diagnostics ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 977
Author(s):  
Jaehyung Jeon ◽  
Chanyong Park ◽  
Dinesh Veeran Ponnuvelu ◽  
Sungsu Park
Keyword(s):  
Exposure Time ◽  
Point Of Care ◽  
Three Dimensional ◽  
Point Of Care Testing ◽  
Detection Zone ◽  
Enhanced Sensitivity ◽  
Gray Intensity ◽  
Novel Method ◽  
Insight Into ◽  
Higher Sensitivity

Despite the potential in fabrication of microfluidic paper-based analytical devices (μPADs) for point-of-care testing (POCT) kits, the development of simple, accurate, and rapid devices with higher sensitivity remains challenging. Here, we report a novel method for 3D-μPAD fabrication with enclosed channels using vat photopolymerization to avoid fluid evaporation. In detail, height of the enclosed channels was adjusted from 0.3 to 0.17 mm by varying the UV exposure time from 1 to 4 s for the top barrier, whereas the exposure time for the bottom and side barriers was fixed. As a result, sample flow in the enclosed channels of 3D-μPADs showed lesser wicking speed with very scant evaporation compared to that in the hemi channels in the 3D-μPADs. The stoppage of evaporation in the enclosed channels significantly improved the gray intensity and uniformity in the detection zone of the 3D-μPADs, resulting in as low as 0.3 mM glucose detection. Thus 3D-μPADs with enclosed channels showed enhanced sensitivity compared to the 3D-μPADs with hemi channels when dealing with a small volume sample. Our work provides a new insight into 3D-μPAD design with enclosed channels, which redefines the methodology in 3D printing.

A three-dimensional origami-based immuno-biofuel cell for self-powered, low-cost, and sensitive point-of-care testing

2014 ◽  
Vol 50 (16) ◽  
pp. 1947 ◽  
Author(s):  
Yanhu Wang ◽  
Lei Ge ◽  
Panpan Wang ◽  
Mei Yan ◽  
Jinghua Yu ◽  
...  
Keyword(s):  
Point Of Care ◽  
Low Cost ◽  
Three Dimensional ◽  
Biofuel Cell ◽  
Point Of Care Testing ◽  
Self Powered

scholarly journals Three-Dimensional Paper-Based Microfluidic Analysis Device for Simultaneous Detection of Multiple Biomarkers With a Smartphone

2020 ◽  
Author(s):  
Seung Ho Baek ◽  
Chanyong Park ◽  
Jaehyung Jeon ◽  
Sungsu Park
Keyword(s):  
Point Of Care ◽  
Simultaneous Detection ◽  
Three Dimensional ◽  
Cost Effective ◽  
Smartphone Application ◽  
Accurate Diagnosis ◽  
Point Of Care Testing ◽  
Digital Light Processing ◽  
Multiple Biomarkers ◽  
Microfluidic Analysis

Paper-based microfluidic analysis devices (μPADs) have attracted attention as a cost-effective platform for point-of-care testing (POCT), food safety, and environmental monitoring. Recently, three-dimensional (3D)-μPADs have been developed to improve the performance of μPADs. For accurate diagnosis of diseases, however, 3D-μPADs need to be developed to simultaneously detect multiple biomarkers. Here, we report a 3D-μPADs platform for the detection of multiple biomarkers that can be analyzed and diagnosed with a smartphone. The 3D-μPADs were fabricated using a 3D digital light processing printer and consisted of a sample reservoir (300 µL) connected to 24 detection zones (of 4 mm in diameter) through 8 microchannels (of 2 mm in width). With the smartphone application, eight different biomarkers related to various diseases were detectable in concentrations ranging from normal to abnormal conditions: glucose (0–20 mmol/L), cholesterol (0–10 mmol/L), albumin (0–7 g/dL), alkaline phosphatase (0–800 U/L), creatinine (0–500 µmol/L), aspartate aminotransferase (0–800 U/L), alanine aminotransferase (0–1000 U/L), and urea nitrogen (0–7.2 mmol/L). These results suggest that 3D-µPADs can be used as a POCT platform for simultaneous detection of multiple biomarkers.

Three-dimensional paper-based electrochemiluminescence immunodevice for multiplexed measurement of biomarkers and point-of-care testing

Biomaterials ◽  
2012 ◽  
Vol 33 (4) ◽  
pp. 1024-1031 ◽  
Author(s):  
Lei Ge ◽  
Jixian Yan ◽  
Xianrang Song ◽  
Mei Yan ◽  
Shenguang Ge ◽  
...  
Keyword(s):  
Point Of Care ◽  
Three Dimensional ◽  
Point Of Care Testing

Electrochemical K-562 cells sensor based on origami paper device for point-of-care testing

Talanta ◽  
2015 ◽  
Vol 145 ◽  
pp. 12-19 ◽  
Author(s):  
Shenguang Ge ◽  
Lina Zhang ◽  
Yan Zhang ◽  
Haiyun Liu ◽  
Jiadong Huang ◽  
...  
Keyword(s):  
Point Of Care ◽  
Point Of Care Testing ◽  
Paper Device ◽  
K 562 Cells
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