Enhanced Sensing Behavior of Three-Dimensional Microfluidic Paper-Based Analytical Devices (3D-μPADs) with Evaporation-Free Enclosed Channels for Point-of-Care Testing

Despite the potential in fabrication of microfluidic paper-based analytical devices (μPADs) for point-of-care testing (POCT) kits, the development of simple, accurate, and rapid devices with higher sensitivity remains challenging. Here, we report a novel method for 3D-μPAD fabrication with enclosed channels using vat photopolymerization to avoid fluid evaporation. In detail, height of the enclosed channels was adjusted from 0.3 to 0.17 mm by varying the UV exposure time from 1 to 4 s for the top barrier, whereas the exposure time for the bottom and side barriers was fixed. As a result, sample flow in the enclosed channels of 3D-μPADs showed lesser wicking speed with very scant evaporation compared to that in the hemi channels in the 3D-μPADs. The stoppage of evaporation in the enclosed channels significantly improved the gray intensity and uniformity in the detection zone of the 3D-μPADs, resulting in as low as 0.3 mM glucose detection. Thus 3D-μPADs with enclosed channels showed enhanced sensitivity compared to the 3D-μPADs with hemi channels when dealing with a small volume sample. Our work provides a new insight into 3D-μPAD design with enclosed channels, which redefines the methodology in 3D printing.

Review on Buildings Health Monitoring System by Using Hybrid Data Optimization Based on Machine Learning Algorithm

The detection of cracks is a crucial task in monitoring structural health and ensuring structural safety. The manual process of crack detection is pains takingly time-consuming and suffers from subjective judgments of inspectors. This study establishes an intelligent model based on image processing techniques for automatic crack recognition and analyses. In the new model, a gray intensity adjustment method, called Min-Max Gray Level Discrimination (M2GLD), is proposed to preprocess the image thresholded by the Otsu method. The goal of this gray intensity adjustment method is to meliorate the accuracy of the crack detection results. Experimental results point out that the integration of M2GLD and the Otsu method, followed by other shape analysis algorithms, can successfully detect crack defects in digital images. Therefore, the constructed model can be a useful tool for building management agencies and construction engineers in the task of structure maintenance.

Do Grading Gray Stimuli Help to Encode Letter Position?

Numerous experiments in the past decades recurrently showed that a transposed-letter pseudoword (e.g., JUGDE) is much more wordlike than a replacement-letter control (e.g., JUPTE). Critically, there is an ongoing debate as to whether this effect arises at a perceptual level (e.g., perceptual uncertainty at assigning letter position of an array of visual objects) or at an abstract language-specific level (e.g., via a level of “open bigrams” between the letter and word levels). Here, we designed an experiment to test the limits of perceptual accounts of letter position coding. The stimuli in a lexical decision task were presented either with a homogeneous letter intensity or with a graded gray intensity, which indicated an unambiguous letter order. The pseudowords were either transposed-letter pseudowords or replaced-letter pseudowords (e.g., jugde vs. jupte). The results showed much longer response times and substantially more errors in the transposed-letter pseudowords than in the replacement-letter pseudowords, regardless of visual format. These findings favor the idea that language-specific orthographic element factors play an essential role when encoding letter position during word recognition.

Bee Venom Stimulates Hormone Secretion in Rat Somatotroph and Corticotroph Cells: Digital Image Analysis of Secretory Granules

In this study, we characterized secretory granules in somatotroph (STCs) and corticotroph (CTCs) cells from the anterior pituitary of rats, in conjunction with different experimental treatments with bee venom (BV). In the rats injected for 30 days with daily BV doses equivalent to one sting, we found significant changes in secretory granules' diameter: reduced by 48.15% in STCs and increased by 5.09% in CTCs, and especially a shift to gray into their intensity profile: increased by 237.04% in STCs and by 212.38% in CTCs. In the rats injected with a single high BV dose, the granules' diameter was reduced in both STCs (by 7.14%) and CTCs (by 4.67%—significant) and their gray intensity profile increased by 200% in STCs and by 51.71% in CTCs (both are significant). The changes in the gray profile reflected a reduced content of granules in the cells, consistent with an increase of the plasma levels of GH and ACTH in all cases. We concluded that the reduced hormone cargo of granules in STCs and CTCs resulted from an accelerated cell secretion. The results obtained for the two types of cells correlated, indicating a similar reaction of these secretory cells to the prolonged and acute presence of BV in the organism.

Experimental Investigation of Purging Saline Solution from a Dead-End Water Pipe

Salty groundwater might find its way into dead end legs of a water distribution network and thus efforts are required to clean such parts of the network. This paper reports, for the first time, the results of a visual study for laboratory experimental investigation on the purging process of saline water from a dead-end water pipe using fresh water. Three purging locations and a number of purging flow rates were considered to identify the effect of purging location and purging flow rate on the time required to completely remove saline water from the dead-end pipe. Image processing analysis techniques were used to capture data from the experimental lab setup. A universal gray-intensity to salinity curve was experimentally found to formulate a color intensity to salinity mapping. A script code based on Octave numerical package was written for this regard to determine the temporal variation of the total dissolved salt (TDS) value within the dead leg pipe. It is generally noted that, as Reynolds number gets higher, the time removal ratio (t/ts) gets bigger. It is also noted that, as a purging location gets farther from the dead end, the time required for the complete removal of TDS increases exponentially.

Detection of Surface Crack in Building Structures Using Image Processing Technique with an Improved Otsu Method for Image Thresholding

The detection of cracks is a crucial task in monitoring structural health and ensuring structural safety. The manual process of crack detection is painstakingly time-consuming and suffers from subjective judgments of inspectors. This study establishes an intelligent model based on image processing techniques for automatic crack recognition and analyses. In the new model, a gray intensity adjustment method, called Min-Max Gray Level Discrimination (M2GLD), is proposed to preprocess the image thresholded by the Otsu method. The goal of this gray intensity adjustment method is to meliorate the accuracy of the crack detection results. Experimental results point out that the integration of M2GLD and the Otsu method, followed by other shape analysis algorithms, can successfully detect crack defects in digital images. Therefore, the constructed model can be a useful tool for building management agencies and construction engineers in the task of structure maintenance.

165 CRYOPRESERVATION OF IN VITRO PRODUCED BOVINE EMBRYOS AFTER LIPID DECREASE WITH FORSKOLIN

Forskolin® (F-6886) is being used to induce lipolysis and increase cryotolerance, to be an activator of adenylate cyclase, and elevating the cyclic adenosine monophosphate (cAMP) levels. The objective of this experiment was to induce the chemical lipolysis of embryos to improve vitrification and the hypothesis would be that Forskolin decrease the amount of lipid droplets, improve the production of blastocysts, and increase the survival rate after vitrification and warming. Eight random effect were performed which oocytes (N = 1172) were matured in TCM 199® supplemented with 10% of fetal bovine serum (FBS), under 5% CO2 atmosphere, at a temperature of 38.5°C and absolute humidity for 24 h. Semen was selected by Percoll gradient with a final concentration of the 2 × 106 sperm mL–1. Presumptive zygotes were cultured in SOFaa and 2.5% of FBS and were kept in an incubator with 5% CO2, 5% O2 and 90% N2 at 38.5°C and absolute humidity until Day 6, when Forskolin was added and remained until Day 7; control (group without Forskolin); F 2.5 µM (group with 2.5 µM Forskolin); F 5 µM (group with 5 µM Forskolin). On Day 7 (Day 0 = IVF) the rate of blastocyst formation was observed then they were vitrified. Apoptosis was analysed using the TUNEL technique, and the lipid content analysis was performed with Sudan Black B® (S-0395). To estimate the lipid content of embryos, 1 photo at a blastocyst group was performed and submitted to the program ImageJ 1.14 (Wayne Rasband, National Institutes of Health, Bethesda, MD, USA). The embryos were limited to obtain the area (μm2), and gray intensity mean (arbitrary units), and gray intensity per area was calculated (arbitrary units/μm2). Data were analysed by ANOVA with PROC GLM of SAS (SAS Institute, Cary, NC, USA). Sources of variation in the model including treatment and replicas were regarded as fixed and random effects, respectively. Data are presented as mean and standard least-squares error. For all analyzes was adopted the significance level of 5%. There was no difference in blastocyst rate: control (37.0 ± 4.0%), F 2.5 μM (38.6 ± 4.0%), F 5 μM (40.7 ± 4.0%). There were difference in lipids content between all groups: control (136.8 ± 2.2ab); F 2.5 μM (128.5 ± 2.2b), F 5 μM (135.6 ± 2.3c; P < 0.05). The F 2.5 μM group showed the higher rate of apoptotic cells compared to other groups: control (12.1 ± 3.5%a), F 2.5 μM (16.7 ± 4.1%b), F 5 μM (11.1 ± 6.5%a; P < 0.05). After vitrification, there was no difference in re-expansion: control (71.3 ± 8.9%), F 2.5 μM (73.1 ± 8.9%); F 5 μM (66.1 ± 8.9%) and apoptosis rate: control (22.3 ± 3.1%); F 2.5 μM (37.3 ± 3.8%); F 5 μM (33.2 ± 6.5%) between the groups. The Forskolin was effective at lower concentration to diminish lipids concentrations in embryos. But when we analysed the apoptotic cell, the lower concentration of Forskolin damaged embryos, but this effect could be diminished after vitrification and warming, when the drug did not increase the apoptotic cells. However, we need to study other concentrations of Forskolin. FAPESP (2010/50410–2/2014/21289–1) is acknowledged for support.