The dose-dependent antioxidant activity of gallic acid-loaded nanoparticles on HepG2 cell line

2011 ◽  
Vol 22 ◽  
pp. S122
Author(s):  
Mukerrem Betul Yerer Aycan ◽  
Canan Aslan ◽  
Cigdem Yucel ◽  
Merve Inanir ◽  
Yesim Aktas
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Hepg2 Cell Line ◽  
Dose Dependent

scholarly journals Phytoconstituents and bioactivities of the Bark of Pleiogynium timorense (DC.) Leenh (Anacardiaceae)

2020 ◽  
Vol 9 (1) ◽  
pp. 20-27
Author(s):  
Gehan Fawzy Abdel Raoof ◽  
Ataa Abdelhaleem Said ◽  
Khaled Younes Mohamed ◽  
Hesham A. Gomaa
Keyword(s):  
Phenolic Compounds ◽  
Gallic Acid ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Cytotoxic Effect ◽  
Methanol Extract ◽  
Human Colon ◽  
Positive Control ◽  
Liver Carcinoma ◽  
Hepg2 Cell Line

Introduction: The purpose of this study was to evaluate the phytoconstituents and various bioactivities of Pleiogynium timorense bark as a step towards the production of a new drug from natural origin to overcome the complications of the synthetic drugs. Methods: The phenolic compounds were isolated and identified by chromatographic and spectroscopic methods as ultraviolet (UV) and nuclear magnetic resonance (NMR) spectra. The isolated compounds, as well as 70% methanol extract of P. timorense bark were tested for cytotoxicity against human colon carcinoma (HCT 116), human hepatocellular liver carcinoma (HepG2), normal melanocytes (HFB-4) and human breast carcinoma (MCF-7) cell lines. In addition, the methanol extract was evaluated for renal protective, hepatoprotective, antioxidant and antihyperglycaemic activities. Results: Seven phenolic compounds were isolated from the bark of the plant for the first time which were identified as; pyrogallol, catechin, gallic acid, kaempferol, quercetin, rutin and quercetrin. Moreover, the methanol extract of the bark showed a promising cytotoxic effect against HepG2 cell line more than that of the isolated compounds comparing with doxorubicin (a positive control), where catechin and gallic acid showed moderate effects. In addition, the methanol extract showed potent antioxidant, hepatorenal protective and antihyperglycaemic effects. Conclusion: Pleiogynium timorense extract possesses a potent cytotoxic effect against HepG2 cell line and significant antioxidant, hepatorenal protective and antihyperglycaemic effects.

Chemopreventive and hepatoprotective roles of adiponectin (SULF2 inhibitor) in hepatocelluar carcinoma

2016 ◽  
Vol 397 (3) ◽  
pp. 257-267 ◽  
Author(s):  
Mohammed M.H. Al-Gayyar ◽  
Ahmed Abbas ◽  
Ahmed M. Hamdan
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Extracellular Enzyme ◽  
Dependent Manner ◽  
Hepg2 Cell Line ◽  
Tnf Α ◽  
Dose Dependent

Abstract Sulfatase 2 (SULF2) is an extracellular enzyme that catalyzes the removal of 6-O-sulfate groups from the heparan sulfate (HS). As elevated SULF2 activity has been correlated with hepatocellular carcinoma (HCC), this study was conducted to evaluate the chemoprotective and the hepatoprotective roles of adiponectin, as a SULF2 inhibitor, against hepatocellular carcinoma both in vivo and in vitro. HCC was induced in rats using thioacetamide (200 mg/kg). Treated rats received adiponectin (5 μg/kg) once a week. Moreover, human hepatocellular carcinoma (HepG2) cell line was used as an in-vitro model. In both in-vivo and in-vitro models, adiponectin completely blocked HCC-induced SULF2 elevation. The antitumor activity of adiponectin was confirmed by 80% increased the survival rate, 73% reduction in the average number of nodules per nodule-bearing liver and 46% reduction in serum AFP. In addition, adiponectin ameliorated HCC-induced expression of tumor invasion markers, MMP9, syndecan-1 and FGF-2. Moreover, adiponectin attenuated HCC-induced elevation of nfκb and TNF-α levels. Moreover, treatment of HepG2 cell line with adiponectin showed dose-dependent reduction of HepG2 cell viability and elevation of cellular cytotoxicity. Besides, Adiponectin yielded the same results in HepG2 cells in a dose-dependent manner. Adiponectin achieved both hepatoprotective and chemoprotective effects against HCC through blocking of SULF2.

scholarly journals Metformin attenuates hypoxia‑induced resistance to cisplatin in the HepG2 cell line

2018 ◽  
Author(s):  
Hiromasa Fujita ◽  
Katsumi Hirose ◽  
Mariko Sato ◽  
Ichitaro Fujioka ◽  
Tamaki Fujita ◽  
...  
Keyword(s):  
Cell Line ◽  
Hepg2 Cell ◽  
Induced Resistance ◽  
Hepg2 Cell Line

Preliminary screening of some traditional Zulu medicinal plants for antineoplastic activities versus the HepG2 cell line

2000 ◽  
Vol 14 (7) ◽  
pp. 534-537 ◽  
Author(s):  
A. R. Opoku ◽  
M. Geheeb-Keller ◽  
J. Lin ◽  
S. E. Terblanche ◽  
A. Hutchings ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Hepg2 Cell Line ◽  
Preliminary Screening

scholarly journals Isolation and identification of heterogenic subpopulation from the HepG2 cell line by Rhodamine123 extrusion assay

2008 ◽  
Vol 16 (9) ◽  
pp. 941
Author(s):  
Nian-Song Qian ◽  
Ren Li ◽  
Heng-Chao Yu ◽  
Yun-Xin Cao ◽  
Fu-Qin Zhang ◽  
...  
Keyword(s):  
Cell Line ◽  
Hepg2 Cell ◽  
Hepg2 Cell Line ◽  
Isolation And Identification

scholarly journals Atomic force microscopy correlates antimetastatic potentials of HepG2 cell line with its redox/energy status: effects of curcumin and Khaya senegalensis

2017 ◽  
Vol 15 (3) ◽  
pp. 214-230 ◽  
Author(s):  
Jeremiah Olorunjuwon Olugbami ◽  
Robert Damoiseaux ◽  
Bryan France ◽  
Michael A. Gbadegesin ◽  
Adam Z. Stieg ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Hepg2 Cell Line ◽  
Energy Status ◽  
Force Microscopy ◽  
Atomic Force ◽  
Khaya Senegalensis
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