scholarly journals Development of high-performance liquid chromatographic fingerprints for distinguishing Chinese Angelica from related umbelliferae herbs

2005 ◽  
Vol 1073 (1-2) ◽  
pp. 383-392 ◽  
Author(s):  
Guang-Hua Lu ◽  
Kelvin Chan ◽  
Yi-Zeng Liang ◽  
Kelvin Leung ◽  
Chi-Leung Chan ◽  
...  
Keyword(s):  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Chromatographic Fingerprints ◽  
Liquid Chromatographic

scholarly journals Studies on Chromatographic Fingerprint and Fingerprinting Profile-Efficacy Relationship of Polygoni Perfoliati Herba

2013 ◽  
Vol 2013 ◽  
pp. 1-15 ◽  
Author(s):  
Li Tian ◽  
Yang Zhao ◽  
Xin Zhou ◽  
Hua-Guo Chen ◽  
Chao Zhao ◽  
...  
Keyword(s):  
Acetic Acid ◽  
High Performance ◽  
Principal Component ◽  
High Performance Liquid Chromatographic ◽  
Chromatographic Fingerprints ◽  
Peritoneal Permeability ◽  
Anti Inflammatory ◽  
Relationship Of ◽  
Liquid Chromatographic ◽  
The Relationship

Polygoni Perfoliati Herba is widely used in China with antibacterium, anti-inflammatory, expectorant, antitumor, and antivirus activities. To reveal the mechanisms of the activities of Polygoni Perfoliati Herba, the relationship between the fingerprinting profile and its bioactivities was investigated. In the present study, high-performance liquid chromatographic (HPLC) fingerprinting method was developed. The established method was applied to analyze 51 batches of Polygoni Perfoliati Herba samples collected from different locations or in different harvesting times in China. Chemometrics, including similarity analysis, hierarchical clustering analysis, and principal component analysis, were used to express their similarities. It was found that similarity values of the samples were in the range of 0.432–0.998. The results of analgesic tests indicated that Polygoni Perfoliati Herba could significantly inhibit pain induced by hot plate and acetic acid in mice. The results of anti-inflammatory tests showed that Polygoni Perfoliati Herba had good anti-inflammatory effects (P< 0.01) in two models including dimethyl benzene-induced ear edema and acetic acid-induced peritoneal permeability in mice. Combining the results from chromatographic fingerprints with those from bioactivities, we found that seven peaks from Polygoni Perfoliati Herba were mainly responsible for analgesic and anti-inflammatory activities.

scholarly journals Chromatographic Fingerprint Analysis of Pycnogenol® Dietary Supplements

2009 ◽  
Vol 92 (2) ◽  
pp. 624-632 ◽  
Author(s):  
Pei Chen ◽  
Fenhong Song ◽  
Long-Ze Lin
Keyword(s):  
Dietary Supplements ◽  
High Performance ◽  
Principal Component ◽  
The United States ◽  
High Performance Liquid Chromatographic ◽  
Hplc Method ◽  
Fingerprint Analysis ◽  
Chromatographic Fingerprints ◽  
Peak Areas ◽  
Liquid Chromatographic

Abstract The bark of maritime pine (Pinus pinaster Aiton) has been widely used as a remedy for various degenerative diseases. A standard high-performance liquid chromatographic (HPLC) procedure for Pycnogenol® analysis is a method specified in the United States Pharmacopeia (USP) monograph, which requires measurement of peak areas and identification of four components of the extract: caffeic acid, catechin, ferulic acid, and taxifolin. In this study, a fingerprint analysis using an HPLC method based on the USP monograph has been developed to provide additional qualitative information for the analysis of Pycnogenol-containing dietary supplements (PDS). Twelve commercially available PDS samples were purchased and analyzed along with a standard Pycnogenol extract. Their chromatographic fingerprints were analyzed using principal component analysis. The results showed that two of the samples were not consistent with the standard reference Pycnogenol extract. One contained other active ingredients in addition to Pycnogenol, and the other may have resulted from a quality control issue in manufacturing.

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