scholarly journals Studies on Chromatographic Fingerprint and Fingerprinting Profile-Efficacy Relationship of Polygoni Perfoliati Herba

2013 ◽  
Vol 2013 ◽  
pp. 1-15 ◽  
Author(s):  
Li Tian ◽  
Yang Zhao ◽  
Xin Zhou ◽  
Hua-Guo Chen ◽  
Chao Zhao ◽  
...  
Keyword(s):  
Acetic Acid ◽  
High Performance ◽  
Principal Component ◽  
High Performance Liquid Chromatographic ◽  
Chromatographic Fingerprints ◽  
Peritoneal Permeability ◽  
Anti Inflammatory ◽  
Relationship Of ◽  
Liquid Chromatographic ◽  
The Relationship

Polygoni Perfoliati Herba is widely used in China with antibacterium, anti-inflammatory, expectorant, antitumor, and antivirus activities. To reveal the mechanisms of the activities of Polygoni Perfoliati Herba, the relationship between the fingerprinting profile and its bioactivities was investigated. In the present study, high-performance liquid chromatographic (HPLC) fingerprinting method was developed. The established method was applied to analyze 51 batches of Polygoni Perfoliati Herba samples collected from different locations or in different harvesting times in China. Chemometrics, including similarity analysis, hierarchical clustering analysis, and principal component analysis, were used to express their similarities. It was found that similarity values of the samples were in the range of 0.432–0.998. The results of analgesic tests indicated that Polygoni Perfoliati Herba could significantly inhibit pain induced by hot plate and acetic acid in mice. The results of anti-inflammatory tests showed that Polygoni Perfoliati Herba had good anti-inflammatory effects (P< 0.01) in two models including dimethyl benzene-induced ear edema and acetic acid-induced peritoneal permeability in mice. Combining the results from chromatographic fingerprints with those from bioactivities, we found that seven peaks from Polygoni Perfoliati Herba were mainly responsible for analgesic and anti-inflammatory activities.

scholarly journals Chromatographic Fingerprint Analysis of Pycnogenol® Dietary Supplements

2009 ◽  
Vol 92 (2) ◽  
pp. 624-632 ◽  
Author(s):  
Pei Chen ◽  
Fenhong Song ◽  
Long-Ze Lin
Keyword(s):  
Dietary Supplements ◽  
High Performance ◽  
Principal Component ◽  
The United States ◽  
High Performance Liquid Chromatographic ◽  
Hplc Method ◽  
Fingerprint Analysis ◽  
Chromatographic Fingerprints ◽  
Peak Areas ◽  
Liquid Chromatographic

Abstract The bark of maritime pine (Pinus pinaster Aiton) has been widely used as a remedy for various degenerative diseases. A standard high-performance liquid chromatographic (HPLC) procedure for Pycnogenol® analysis is a method specified in the United States Pharmacopeia (USP) monograph, which requires measurement of peak areas and identification of four components of the extract: caffeic acid, catechin, ferulic acid, and taxifolin. In this study, a fingerprint analysis using an HPLC method based on the USP monograph has been developed to provide additional qualitative information for the analysis of Pycnogenol-containing dietary supplements (PDS). Twelve commercially available PDS samples were purchased and analyzed along with a standard Pycnogenol extract. Their chromatographic fingerprints were analyzed using principal component analysis. The results showed that two of the samples were not consistent with the standard reference Pycnogenol extract. One contained other active ingredients in addition to Pycnogenol, and the other may have resulted from a quality control issue in manufacturing.

Characterization of the photoautotrophic algal and bacterial communities in a large, shallow, subtropical lake using HPLC-PDA based pigment analysis

1998 ◽  
Vol 55 (1) ◽  
pp. 206-219 ◽  
Author(s):  
Alan D Steinman ◽  
Karl E Havens ◽  
J William Louda ◽  
Nancy M Winfree ◽  
Earl W Baker
Keyword(s):  
Water Column ◽  
Chlorophyll A ◽  
High Performance ◽  
Principal Component ◽  
Open Water ◽  
High Performance Liquid Chromatographic ◽  
Lake Okeechobee ◽  
Pheophytin A ◽  
Pigment Analysis ◽  
Liquid Chromatographic

Pigment abundances of the oxygenic and anoxygenic photoautotrophic communities from sediments and the water column in Lake Okeechobee, Florida, were estimated using reverse-phase high-performance liquid chromatographic (RP-HPLC) and photodiode array (PDA) UV/VIS (350-800 nm) spectrophotometric analyses. Thirty lipophilic pigments were identified and measured in the samples, with the most abundant overall (sediment and open-water samples combined) being chlorophyll a (38.1%), fucoxanthin (12.6%), pheophytin a (7.6%), zeaxanthin (6.6%), and pyropheophytin a (3.6%). Relative abundance of chlorophyll a was greater in the water column than in the sediments (58.3 versus 24.3% of all pigments) whereas pheophytin a comprised 9.1% of the total pigments in the sediments but only 3.7% of the total pigments in the water column. Principal component analysis (PCA) separated the sediment samples from those collected in the water column; this discrimination appears to be a function of pigment integrity in that sediment assemblages had much greater relative abundances of degraded pigments. Different regions of the lake were weakly separated by PCA based on pigments. The relatively weak degree of separation may reflect the overwhelming abundance of chlorophyll a at all sites. Overall, the pigment assemblage in Lake Okeechobee suggests cyanobacteria-diatom dominance. Out of 65 sampling events, pigments from anoxygenic photoautotrophs (e.g., bacteriochlorophylls) were detected 17 times but accounted for >20% of total chlorophyll only five times. Bacteriochlorophylls were observed only in the sediments and were most abundant during June and September, when winds were calm and temperatures warm, and at relatively shallow sites.

scholarly journals RP-HPLC Method Development and Validation for Estimation of Acebrophylline

2019 ◽  
Vol 6 (6) ◽  
pp. 56-59
Author(s):  
Bhavik, Sharma ◽  
Sushil Kumar Agarwal
Keyword(s):  
Acetic Acid ◽  
High Performance ◽  
Method Development ◽  
High Performance Liquid Chromatographic ◽  
Hplc Method ◽  
Blood Levels ◽  
Rp Hplc ◽  
Economic Method ◽  
Liquid Chromatographic

Acebrophylline is an anti-inflammatory and airway mucus regulator. It had ambroxol and theophylline-7-acetic acid, the former facilitates the biosynthesis of pulmonary surfactant which raises blood levels of ambroxol, by stimulating surfactant production. Chemical structure of acebrophylline is 1, 2, 3, 6- tetrahydro-1, 3-dimethyl-2, 6-dioxo-7H-purine-7-aceticacidwithtrans-4-[(2-amino-3, 5 dibromophenyl) methyl] aminio] cyclohexanol. Survey revealed that various analytical methods like spectrophotometric, HPLC, and RP-HPLC, have been reported for the determination of Ambroxol HCl and Theophylline-7-acetic acid, individually and in combination with some other drugs. The aim of present study was to develop and validate stability indicating HPLC method for the analyses of acebrophylline. High performance liquid chromatographic method has been developed for the estimation of Acebrophylline. Reported methods also include RP-HPLC method for determination of Acebrophylline. The developed UV spectrophotometric method is simple and requires less time for the analysis. It is also rapid and economic method.

Paired-Ion Chromatography and High Performance Liquid Chromatography of Labetalol in Feeds

1980 ◽  
Vol 63 (6) ◽  
pp. 1262-1265
Author(s):  
Edward R Townley ◽  
Bruce Ross
Keyword(s):  
Acetic Acid ◽  
Ion Chromatography ◽  
High Performance ◽  
Blocking Agent ◽  
High Performance Liquid Chromatographic ◽  
Hplc Method ◽  
Beta Adrenoceptor ◽  
Insoluble Material ◽  
Relative Standard ◽  
Liquid Chromatographic

Abstract A high performance liquid chromatographic (HPLC) method using reverse phase paired-ion chromatography and ultraviolet detection at 280 nm has been developed to determine labetalol, an alpha and beta adrenoceptor blocking agent, in Purina No. 5001 rodent chow. The method is simple and rapid, and demonstrates a separation technique applicable to other acidic and basic drugs. It requires only extraction of the drug with methanol–water–acetic acid (66+33+1) and separation of insoluble material by filtration before HPLC. Labetalol, is chromatographically separated from soluble feed components by means of a µBondapak C18 column and methanol–water–acetic acid (66+33+1) mobile phase, 0.005M with respect to sodium dioctylsulfosuccinate pairedion reagent. Average recovery is 98.7% with a relative standard deviation of ±2.3% for the equipment described.

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