Protein–lipid interactions and surface activity in the pulmonary surfactant system

2006 ◽  
Vol 141 (1-2) ◽  
pp. 105-118 ◽  
Author(s):  
Alicia G. Serrano ◽  
Jesús Pérez-Gil
Keyword(s):  
Surface Activity ◽  
Pulmonary Surfactant ◽  
Lipid Interactions ◽  
Surfactant System

Perinatal sidestream cigarette smoke exposure and the developing pulmonary surfactant system in rats

1999 ◽  
Vol 18 (4) ◽  
pp. 206-211 ◽  
Author(s):  
Sriram Subramaniaml ◽  
Suseela Srinivasan ◽  
Paul M Bummer ◽  
C Gary Gairola
Keyword(s):  
Cigarette Smoke ◽  
Surface Activity ◽  
Pulmonary Surfactant ◽  
Whole Body ◽  
Cigarette Smoke Exposure ◽  
Perinatal Exposure ◽  
Sprague Dawley ◽  
Adult Rats ◽  
Surfactant System ◽  
Sidestream Smoke

1 Epidemiological studies have strongly implicated passive smoking with increased incidence of various respiratory diseases in children. Our earlier studies have shown that chronic exposure to tobacco smoke significantly changes the composition and the surface activity of the pulmonary surfactant in adult rats. The aim of the present study was to determine if perinatal exposure to sidestream cigarette smoke influences the composition and function of pulmonary surfactant system in developing rat pups. 2 Pregnant Sprague Dawley rats were exposed to sidestream cigarette smoke in a whole body exposure chamber for a total of 6 h each day and the pups born to these dams were further exposed to sidestream smoke for 2 h/day during postnatal period. Exposure of animals to smoke was ascertained by measuring their plasma cotinine. Surfactant analysis was performed on bronchoalveolar lavage fluids (BALF) collected from pups on postnatal day 1, 3, 7, 14, 21 and 35. The phospholipid (PL) content, percentage disaturated phosphatidylcholine (DSPC) and surfactant proteins (SP-A and SP-B) in BALF surfactant preparations from sham and smoke-exposed pups were compared. 3 Significant differences between the two groups were observed at two exposure points: a reduced level of SPA on day 1, and, a higher level of SP-A and PLs on day 21, in smoke-exposed pups. Surface activity analysis of the surfactant films by pulsating bubble surfactometer did not show any significant differences between the sham and smoke-exposed groups at any exposure point. 4 The results indicate that perinatal exposure to sidestream smoke is capable of producing biochemical changes in the composition of pulmonary surfactant at different stages of development but these changes do not influence surface tension reducing properties of the surfactant.

scholarly journals Palmitoylation as a Key Factor to Understand Sp-C-Lipid Interactions in the Lung Surfactant System

2014 ◽  
Vol 106 (2) ◽  
pp. 513a
Author(s):  
Nuria Roldan ◽  
Erik Goormaghtigh ◽  
Jesus Perez-Gil ◽  
Begoña Garcia-Alvarez
Keyword(s):  
Lung Surfactant ◽  
Lipid Interactions ◽  
Surfactant System ◽  
Key Factor

Alterations in the surface properties of lung surfactant in the torpid marsupial Sminthopsis crassicaudata

1998 ◽  
Vol 84 (1) ◽  
pp. 146-156 ◽  
Author(s):  
Olga V. Lopatko ◽  
Sandra Orgeig ◽  
Christopher B. Daniels ◽  
David Palmer
Keyword(s):  
Surface Tension ◽  
Surface Properties ◽  
Surface Activity ◽  
Pulmonary Surfactant ◽  
Lung Surfactant ◽  
Lung Compliance ◽  
Equilibrium Surface Tension ◽  
Sminthopsis Crassicaudata

Lopatko, Olga V., Sandra Orgeig, Christopher B. Daniels, and David Palmer. Alterations in the surface properties of lung surfactant in the torpid marsupial Sminthopsis crassicaudata. J. Appl. Physiol. 84(1): 146–156, 1998.—Torpor changes the composition of pulmonary surfactant (PS) in the dunnart Sminthopsis crassicaudata [C. Langman, S. Orgeig, and C. B. Daniels. Am. J. Physiol. 271 ( Regulatory Integrative Comp. Physiol. 40): R437–R445, 1996]. Here we investigated the surface activity of PS in vitro. Five micrograms of phospholipid per centimeter squared surface area of whole lavage (from mice or from warm-active, 4-, or 8-h torpid dunnarts) were applied dropwise onto the subphase of a Wilhelmy-Langmuir balance at 20°C and stabilized for 20 min. After 4 h of torpor, the adsorption rate increased, and equilibrium surface tension (STeq), minimal surface tension (STmin), and the %area compression required to achieve STmin decreased, compared with the warm-active group. After 8 h of torpor, STmin decreased [from 5.2 ± 0.3 to 4.1 ± 0.3 (SE) mN/m]; %area compression required to achieve STmindecreased (from 43.4 ± 1.0 to 27.4 ± 0.8); the rate of adsorption decreased; and STeqincreased (from 26.3 ± 0.5 to 38.6 ± 1.3 mN/m). ST-area isotherms of warm-active dunnarts and mice at 20°C had a shoulder on compression and a plateau on expansion. These disappeared on the isotherms of torpid dunnarts. Samples of whole lavage (from warm-active and 8-h torpor groups) containing 100 μg phospholipid/ml were studied by using a captive-bubble surfactometer at 37°C. After 8 h of torpor, STmin increased (from 6.4 ± 0.3 to 9.1 ± 0.3 mN/m) and %area compression decreased in the 2nd (from 88.6 ± 1.7 to 82.1 ± 2.0) and 3rd (from 89.1 ± 0.8 to 84.9 ± 1.8) compression-expansion cycles, compared with warm-active dunnarts. ST-area isotherms of warm-active dunnarts at 37°C did not have a shoulder on compression. This shoulder appeared on the isotherms of torpid dunnarts. In conclusion, there is a strong correlation between in vitro changes in surface activity and in vivo changes in lipid composition of PS during torpor, although static lung compliance remained unchanged (see Langman et al. cited above). Surfactant from torpid animals is more active at 20°C and less active at 37°C than that of warm-active animals, which may represent a respiratory adaptation to low body temperatures of torpid dunnarts.

The surface activity of pulmonary surfactant from diving mammals

2006 ◽  
Vol 150 (2-3) ◽  
pp. 220-232 ◽  
Author(s):  
Natalie J. Miller ◽  
Christopher B. Daniels ◽  
Samuel Schürch ◽  
W. Michael Schoel ◽  
Sandra Orgeig
Keyword(s):  
Surface Activity ◽  
Pulmonary Surfactant

Response of the Pulmonary Surfactant System To Phosgene

1985 ◽  
Vol 1 (2) ◽  
pp. 29-35 ◽  
Author(s):  
M.F. Frosolono ◽  
William D. Currie
Keyword(s):  
Pulmonary Surfactant ◽  
Surfactant System

Divalent cation and hydrogen ion effects on the structure and surface activity of pulmonary surfactant

Biochemistry ◽  
1987 ◽  
Vol 26 (24) ◽  
pp. 7986-7993 ◽  
Author(s):  
Hava Efrati ◽  
Samuel Hawgood ◽  
Mary C. Williams ◽  
Keelung Hong ◽  
Bradley J. Benson
Keyword(s):  
Surface Activity ◽  
Divalent Cation ◽  
Pulmonary Surfactant ◽  
Hydrogen Ion ◽  
Ion Effects
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