The surface activity of pulmonary surfactant from diving mammals

2006 ◽  
Vol 150 (2-3) ◽  
pp. 220-232 ◽  
Author(s):  
Natalie J. Miller ◽  
Christopher B. Daniels ◽  
Samuel Schürch ◽  
W. Michael Schoel ◽  
Sandra Orgeig
Keyword(s):  
Surface Activity ◽  
Pulmonary Surfactant

Alterations in the surface properties of lung surfactant in the torpid marsupial Sminthopsis crassicaudata

1998 ◽  
Vol 84 (1) ◽  
pp. 146-156 ◽  
Author(s):  
Olga V. Lopatko ◽  
Sandra Orgeig ◽  
Christopher B. Daniels ◽  
David Palmer
Keyword(s):  
Surface Tension ◽  
Surface Properties ◽  
Surface Activity ◽  
Pulmonary Surfactant ◽  
Lung Surfactant ◽  
Lung Compliance ◽  
Equilibrium Surface Tension ◽  
Sminthopsis Crassicaudata

Lopatko, Olga V., Sandra Orgeig, Christopher B. Daniels, and David Palmer. Alterations in the surface properties of lung surfactant in the torpid marsupial Sminthopsis crassicaudata. J. Appl. Physiol. 84(1): 146–156, 1998.—Torpor changes the composition of pulmonary surfactant (PS) in the dunnart Sminthopsis crassicaudata [C. Langman, S. Orgeig, and C. B. Daniels. Am. J. Physiol. 271 ( Regulatory Integrative Comp. Physiol. 40): R437–R445, 1996]. Here we investigated the surface activity of PS in vitro. Five micrograms of phospholipid per centimeter squared surface area of whole lavage (from mice or from warm-active, 4-, or 8-h torpid dunnarts) were applied dropwise onto the subphase of a Wilhelmy-Langmuir balance at 20°C and stabilized for 20 min. After 4 h of torpor, the adsorption rate increased, and equilibrium surface tension (STeq), minimal surface tension (STmin), and the %area compression required to achieve STmin decreased, compared with the warm-active group. After 8 h of torpor, STmin decreased [from 5.2 ± 0.3 to 4.1 ± 0.3 (SE) mN/m]; %area compression required to achieve STmindecreased (from 43.4 ± 1.0 to 27.4 ± 0.8); the rate of adsorption decreased; and STeqincreased (from 26.3 ± 0.5 to 38.6 ± 1.3 mN/m). ST-area isotherms of warm-active dunnarts and mice at 20°C had a shoulder on compression and a plateau on expansion. These disappeared on the isotherms of torpid dunnarts. Samples of whole lavage (from warm-active and 8-h torpor groups) containing 100 μg phospholipid/ml were studied by using a captive-bubble surfactometer at 37°C. After 8 h of torpor, STmin increased (from 6.4 ± 0.3 to 9.1 ± 0.3 mN/m) and %area compression decreased in the 2nd (from 88.6 ± 1.7 to 82.1 ± 2.0) and 3rd (from 89.1 ± 0.8 to 84.9 ± 1.8) compression-expansion cycles, compared with warm-active dunnarts. ST-area isotherms of warm-active dunnarts at 37°C did not have a shoulder on compression. This shoulder appeared on the isotherms of torpid dunnarts. In conclusion, there is a strong correlation between in vitro changes in surface activity and in vivo changes in lipid composition of PS during torpor, although static lung compliance remained unchanged (see Langman et al. cited above). Surfactant from torpid animals is more active at 20°C and less active at 37°C than that of warm-active animals, which may represent a respiratory adaptation to low body temperatures of torpid dunnarts.

Divalent cation and hydrogen ion effects on the structure and surface activity of pulmonary surfactant

Biochemistry ◽  
1987 ◽  
Vol 26 (24) ◽  
pp. 7986-7993 ◽  
Author(s):  
Hava Efrati ◽  
Samuel Hawgood ◽  
Mary C. Williams ◽  
Keelung Hong ◽  
Bradley J. Benson
Keyword(s):  
Surface Activity ◽  
Divalent Cation ◽  
Pulmonary Surfactant ◽  
Hydrogen Ion ◽  
Ion Effects

Effects of atelectasis on pulmonary surfactant and quasi-static lung mechanics

1965 ◽  
Vol 20 (5) ◽  
pp. 859-864 ◽  
Author(s):  
Bernard E. Levine ◽  
Rudolph P. Johnson
Keyword(s):  
Surface Activity ◽  
Pulmonary Surfactant ◽  
Active Material ◽  
Extraction Procedure ◽  
Left Lung ◽  
Lung Mechanics ◽  
Normal Lung ◽  
Rabbit Lung ◽  
Surfactant Activity

The effects of unilateral atelectasis on pulmonary surfactant activity and lung mechanics have been studied in rabbits. Total left lung collapse was produced by pneumothorax and maintained from 90 min to 8 days. Quasi-static pressure-volume relationships were determined with air and saline after varying intervals. Surface activity of minced lung extracts and of dried foam obtained by tracheal washings was measured on a surface balance. A marked decrease in lung inflatability progressing with duration of collapse was noted during air and saline pressure-volume studies. However, deflation characteristics of all atelectatic lungs were normal. Extracts from minced, in vitro reinflated atelectatic lung showed a variable but significant loss of surface activity compared to the right (normal) lung of the same animal. However, highly surface-active material could be demonstrated in the collapsed lungs by the more efficient extraction procedure of tracheal foaming. Correlation exists therefore between normal volume stability of alveoli after initial inflation and presence of surfactant activity in atelectatic lungs. pressure-volume relationships; rabbit lung surface activity Submitted on July 27, 1964

EVOLUTION OF SURFACE ACTIVITY RELATED FUNCTIONS OF VERTEBRATE PULMONARY SURFACTANT

1998 ◽  
Vol 25 (9) ◽  
pp. 716-721 ◽  
Author(s):  
Christopher B. Daniels ◽  
Olga V. Lopatko ◽  
Sandra Orgeig
Keyword(s):  
Surface Activity ◽  
Pulmonary Surfactant
Sign in / Sign up

Export Citation Format

Share Document